A5U9S9 (ASNA_HAEIE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartate--ammonia ligase
EC=6.3.1.1

Alternative name(s):
Asparagine synthetase A

Gene names

Name:	asnA
Ordered Locus Names:	CGSHiEE_00160

Organism

Haemophilus influenzae (strain PittEE) [Complete proteome] [HAMAP]

Taxonomic identifier

374930 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	330 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + NH3 = AMP + diphosphate + L-asparagine. HAMAP MF_00555
Pathway	Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (ammonia route): step 1/1. HAMAP MF_00555
Subcellular location	Cytoplasm By similarity HAMAP MF_00555.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. AsnA subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Asparagine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	asparagine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW tRNA aminoacylation for protein translation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW aminoacyl-tRNA ligase activity Inferred from electronic annotation. Source: InterPro aspartate-ammonia ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 330	330	Aspartate--ammonia ligase HAMAP MF_00555		PRO_1000017947

Sequences

	Sequence		Length	Mass (Da)
	A5U9S9 [UniParc]. Last modified July 10, 2007. Version 1. Checksum: 2DDD63415A26EF41	FASTA	330	37,422
	10 20 30 40 50 60 MKKTFILQQQ EISFVKNTFT QNLIEQLGII EVQGPILSQV GNGMQDNLSG IEKAVQVNVK 70 80 90 100 110 120 CIPNAVFEVV HSLAKWKRHT LARFNFKEDE GLFVHMKALR PDEDSLDPTH SVYVDQWDWE 130 140 150 160 170 180 KVIPEGRRNF AYLKETVNSI YRAIRLTELA VEARFDIPSI LPKQITFVHS EDLVKRYPDL 190 200 210 220 230 240 SSKERENAIC KEYGAVFLIG IGGKLSDGKP HDGRAPDYDD WTTESENGYK GLNGDILVWN 250 260 270 280 290 300 DQLGKAFELS SMGIRVDQSA LRLQAGLTGD EDRLKMDWHQ DLLNGKLPLT IGGGIGQSRL 310 320 330 AMLLLRKKHI GEVQSSVWPK EMLEEFSNIL

References

[1]

"Characterization and modeling of the Haemophilus influenzae core and supragenomes based on the complete genomic sequences of Rd and 12 clinical nontypeable strains." Hogg J.S., Hu F.Z., Janto B., Boissy R., Hayes J., Keefe R., Post J.C., Ehrlich G.D. Genome Biol. 8:R103.1-R103.18(2007) [PubMed: 17550610] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PittEE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000671 Genomic DNA. Translation: ABQ97530.1.
RefSeq	YP_001289913.1. NC_009566.1.
3D structure databases
ProteinModelPortal	A5U9S9.
SMR	A5U9S9. Positions 5-330.
ModBase	Search...
Protein-protein interaction databases
STRING	A5U9S9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5224731.
GenomeReviews	Gene locus CGSHiEE_00160 in contig CP000671_GR.
KEGG	hip:CGSHiEE_00160.
PATRIC	20276059. VBIHaeInf81350_0032.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2502.
HOGENOM	HBG288146.
OMA	LNDNLNG.
ProtClustDB	PRK05425.
Enzyme and pathway databases
BioCyc	HINF374930:CGSHIEE_00160-MONOMER.
Family and domain databases
HAMAP	MF_00555. AsnA. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II. IPR004618. AsnA. [Graphical view]
KO	K01914.
Pfam	PF03590. AsnA. 1 hit. [Graphical view]
PIRSF	PIRSF001555. Asp_ammon_ligase. 1 hit.
TIGRFAMs	TIGR00669. AsnA. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASNA_HAEIE

Accession

Primary (citable) accession number: A5U9S9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	July 10, 2007
Last modified:	December 14, 2011
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program