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Protein

Cyclopropane mycolic acid synthase 1

Gene

cmaA1

Organism
Mycobacterium tuberculosis (strain ATCC 25177 / H37Ra)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of a double bond to a cyclopropane ring at the distal position of an alpha mycolic acid via the transfer of a methylene group from S-adenosyl-L-methionine. Cyclopropanated mycolic acids are key factors participating in cell envelope permeability, host immunomodulation and persistence.1 Publication

Catalytic activityi

S-adenosyl-L-methionine + phospholipid olefinic fatty acid = S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid.

Pathway:imycolic acid biosynthesis

This protein is involved in the pathway mycolic acid biosynthesis, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway mycolic acid biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei269 – 2691By similarity

GO - Molecular functioni

  • cyclopropane-fatty-acyl-phospholipid synthase activity Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciMTUB419947:GJ8N-3541-MONOMER.
UniPathwayiUPA00915.

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclopropane mycolic acid synthase 1 (EC:2.1.1.79)
Short name:
CMAS
Alternative name(s):
Cyclopropane-fatty-acyl-phospholipid synthase
Short name:
CFA synthase
Short name:
Cyclopropane fatty acid synthase
Mycolic acid methyltransferase
Short name:
MA-MT
S-adenosylmethionine-dependent methyltransferase
Short name:
AdoMet-MT
Short name:
SAM-MT
Gene namesi
Name:cmaA1
Synonyms:cma1
Ordered Locus Names:MRA_3431
OrganismiMycobacterium tuberculosis (strain ATCC 25177 / H37Ra)
Taxonomic identifieri419947 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
ProteomesiUP000001988 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 287287Cyclopropane mycolic acid synthase 1PRO_0000300063Add
BLAST

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliA5U866.
SMRiA5U866. Positions 3-287.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni33 – 342S-adenosyl-L-methionine bindingBy similarity
Regioni68 – 769S-adenosyl-L-methionine bindingBy similarity
Regioni94 – 996S-adenosyl-L-methionine bindingBy similarity
Regioni123 – 1242S-adenosyl-L-methionine bindingBy similarity

Sequence similaritiesi

Belongs to the CFA/CMAS family.Curated

Phylogenomic databases

eggNOGiCOG2230.
HOGENOMiHOG000245191.
KOiK00574.
OMAiANSESPR.
OrthoDBiEOG6BGNZP.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR003333. Mycolic_cyclopropane_synthase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR10108:SF743. PTHR10108:SF743. 1 hit.
PfamiPF02353. CMAS. 1 hit.
[Graphical view]
PIRSFiPIRSF003085. CMAS. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.

Sequencei

Sequence statusi: Complete.

A5U866-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPDELKPHFA NVQAHYDLSD DFFRLFLDPT QTYSCAYFER DDMTLQEAQI
60 70 80 90 100
AKIDLALGKL GLQPGMTLLD VGCGWGATMM RAVEKYDVNV VGLTLSKNQA
110 120 130 140 150
NHVQQLVANS ENLRSKRVLL AGWEQFDEPV DRIVSIGAFE HFGHERYDAF
160 170 180 190 200
FSLAHRLLPA DGVMLLHTIT GLHPKEIHER GLPMSFTFAR FLKFIVTEIF
210 220 230 240 250
PGGRLPSIPM VQECASANGF TVTRVQSLQP HYAKTLDLWS AALQANKGQA
260 270 280
IALQSEEVYE RYMKYLTGCA EMFRIGYIDV NQFTCQK
Length:287
Mass (Da):32,461
Last modified:July 10, 2007 - v1
Checksum:i7E254C15DF5FFF97
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U27357 Genomic DNA. Translation: AAA75624.1.
CP000611 Genomic DNA. Translation: ABQ75216.1.
RefSeqiWP_003900041.1. NC_009525.1.

Genome annotation databases

EnsemblBacteriaiABQ75216; ABQ75216; MRA_3431.
KEGGimra:MRA_3431.
PATRICi18147292. VBIMycTub106795_3831.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U27357 Genomic DNA. Translation: AAA75624.1.
CP000611 Genomic DNA. Translation: ABQ75216.1.
RefSeqiWP_003900041.1. NC_009525.1.

3D structure databases

ProteinModelPortaliA5U866.
SMRiA5U866. Positions 3-287.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABQ75216; ABQ75216; MRA_3431.
KEGGimra:MRA_3431.
PATRICi18147292. VBIMycTub106795_3831.

Phylogenomic databases

eggNOGiCOG2230.
HOGENOMiHOG000245191.
KOiK00574.
OMAiANSESPR.
OrthoDBiEOG6BGNZP.

Enzyme and pathway databases

UniPathwayiUPA00915.
BioCyciMTUB419947:GJ8N-3541-MONOMER.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR003333. Mycolic_cyclopropane_synthase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR10108:SF743. PTHR10108:SF743. 1 hit.
PfamiPF02353. CMAS. 1 hit.
[Graphical view]
PIRSFiPIRSF003085. CMAS. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of a gene involved in the biosynthesis of cyclopropanated mycolic acids in Mycobacterium tuberculosis."
    Yuan Y., Lee R.E., Besra G.S., Belisle J.T., Barry C.E. III
    Proc. Natl. Acad. Sci. U.S.A. 92:6630-6634(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION AS A CYCLOPROPANE SYNTHASE, NOMENCLATURE.
  2. "Genetic basis of virulence attenuation revealed by comparative genomic analysis of Mycobacterium tuberculosis strain H37Ra versus H37Rv."
    Zheng H., Lu L., Wang B., Pu S., Zhang X., Zhu G., Shi W., Zhang L., Wang H., Wang S., Zhao G., Zhang Y.
    PLoS ONE 3:E2375-E2375(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 25177 / H37Ra.

Entry informationi

Entry nameiCMAS1_MYCTA
AccessioniPrimary (citable) accession number: A5U866
Secondary accession number(s): Q11195
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: July 10, 2007
Last modified: July 22, 2015
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.