A5U866 (CMAS1_MYCTA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cyclopropane mycolic acid synthase 1
Short name=CMAS
EC=2.1.1.79

Alternative name(s):
Cyclopropane-fatty-acyl-phospholipid synthase
Short name=CFA synthase
Short name=Cyclopropane fatty acid synthase
Mycolic acid methyltransferase
Short name=MA-MT
S-adenosylmethionine-dependent methyltransferase
Short name=AdoMet-MT
Short name=SAM-MT

Gene names

Name:	cmaA1
Synonyms:	cma1
Ordered Locus Names:	MRA_3431

Organism

Mycobacterium tuberculosis (strain ATCC 25177 / H37Ra) [Complete proteome] [HAMAP]

Taxonomic identifier

419947 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex › Mycobacterium tuberculosis

Protein attributes

Sequence length	287 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the conversion of a double bond to a cyclopropane ring at the distal position of an alpha mycolic acid via the transfer of a methylene group from S-adenosyl-L-methionine. Cyclopropanated mycolic acids are key factors participating in cell envelope permeability, host immunomodulation and persistence. Ref.1
Catalytic activity	S-adenosyl-L-methionine + phospholipid olefinic fatty acid = S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid.
Pathway	Lipid metabolism; mycolic acid biosynthesis.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the CFA/CMAS family.

Ontologies

Keywords
Biological process	Lipid biosynthesis Lipid metabolism
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	lipid biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	cyclopropane-fatty-acyl-phospholipid synthase activity Inferred from mutant phenotype Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 287	287	Cyclopropane mycolic acid synthase 1		PRO_0000300063
Regions
	Region	33 – 34	2	S-adenosyl-L-methionine binding By similarity
	Region	68 – 76	9	S-adenosyl-L-methionine binding By similarity
	Region	94 – 99	6	S-adenosyl-L-methionine binding By similarity
	Region	123 – 124	2	S-adenosyl-L-methionine binding By similarity
Sites
	Active site	269	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5U866 [UniParc]. Last modified July 10, 2007. Version 1. Checksum: 7E254C15DF5FFF97	FASTA	287	32,461
	10 20 30 40 50 60 MPDELKPHFA NVQAHYDLSD DFFRLFLDPT QTYSCAYFER DDMTLQEAQI AKIDLALGKL 70 80 90 100 110 120 GLQPGMTLLD VGCGWGATMM RAVEKYDVNV VGLTLSKNQA NHVQQLVANS ENLRSKRVLL 130 140 150 160 170 180 AGWEQFDEPV DRIVSIGAFE HFGHERYDAF FSLAHRLLPA DGVMLLHTIT GLHPKEIHER 190 200 210 220 230 240 GLPMSFTFAR FLKFIVTEIF PGGRLPSIPM VQECASANGF TVTRVQSLQP HYAKTLDLWS 250 260 270 280 AALQANKGQA IALQSEEVYE RYMKYLTGCA EMFRIGYIDV NQFTCQK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of a gene involved in the biosynthesis of cyclopropanated mycolic acids in Mycobacterium tuberculosis." Yuan Y., Lee R.E., Besra G.S., Belisle J.T., Barry C.E. III Proc. Natl. Acad. Sci. U.S.A. 92:6630-6634(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION AS A CYCLOPROPANE SYNTHASE, NOMENCLATURE.
[2]	"Complete genomic sequence of Mycobacterium tuberculosis strain H37Ra, a non-pathogenic variant closely related to the well-characterized pathogenic strain H37Rv." Wang S.Y., Zheng H.J., Lv L.D., Wang B.F., Zhang X.L., Pu S.Y., Zhu G.F., Wang H.H., Zhao G.P., Zhang Y. Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25177 / H37Ra.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U27357 Genomic DNA. Translation: AAA75624.1. CP000611 Genomic DNA. Translation: ABQ75216.1.
RefSeq	YP_001284778.1. NC_009525.1.
3D structure databases
ProteinModelPortal	A5U866.
SMR	A5U866. Positions 3-287.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABQ75216; ABQ75216; MRA_3431.
GeneID	5214660.
KEGG	mra:MRA_3431.
PATRIC	18147292. VBIMycTub106795_3831.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2230.
HOGENOM	HOG000245191.
KO	K00574.
OMA	QLANINI.
ProtClustDB	CLSK790562.
Enzyme and pathway databases
BioCyc	MTUB419947:GJ8N-3541-MONOMER.
UniPathway	UPA00915.
Family and domain databases
InterPro	IPR003333. Mycolic_cyclopropane_synthase. [Graphical view]
Pfam	PF02353. CMAS. 1 hit. [Graphical view]
PIRSF	PIRSF003085. CMAS. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CMAS1_MYCTA

Accession

Primary (citable) accession number: A5U866
Secondary accession number(s): Q11195

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	July 10, 2007
Last modified:	May 1, 2013
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program