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A5U866 (CMAS1_MYCTA) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 49. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cyclopropane mycolic acid synthase 1

Short name=CMAS
EC=2.1.1.79
Alternative name(s):
Cyclopropane-fatty-acyl-phospholipid synthase
Short name=CFA synthase
Short name=Cyclopropane fatty acid synthase
Mycolic acid methyltransferase
Short name=MA-MT
S-adenosylmethionine-dependent methyltransferase
Short name=AdoMet-MT
Short name=SAM-MT
Gene names
Name:cmaA1
Synonyms:cma1
Ordered Locus Names:MRA_3431
OrganismMycobacterium tuberculosis (strain ATCC 25177 / H37Ra) [Complete proteome] [HAMAP]
Taxonomic identifier419947 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex

Protein attributes

Sequence length287 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the conversion of a double bond to a cyclopropane ring at the distal position of an alpha mycolic acid via the transfer of a methylene group from S-adenosyl-L-methionine. Cyclopropanated mycolic acids are key factors participating in cell envelope permeability, host immunomodulation and persistence. Ref.1

Catalytic activity

S-adenosyl-L-methionine + phospholipid olefinic fatty acid = S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid.

Pathway

Lipid metabolism; mycolic acid biosynthesis.

Subunit structure

Homodimer By similarity.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the CFA/CMAS family.

Ontologies

Keywords
   Biological processLipid biosynthesis
Lipid metabolism
   Cellular componentCytoplasm
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processlipid biosynthetic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functioncyclopropane-fatty-acyl-phospholipid synthase activity

Inferred from mutant phenotype Ref.1. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 287287Cyclopropane mycolic acid synthase 1
PRO_0000300063

Regions

Region33 – 342S-adenosyl-L-methionine binding By similarity
Region68 – 769S-adenosyl-L-methionine binding By similarity
Region94 – 996S-adenosyl-L-methionine binding By similarity
Region123 – 1242S-adenosyl-L-methionine binding By similarity

Sites

Active site2691 By similarity

Sequences

Sequence LengthMass (Da)Tools
A5U866 [UniParc].

Last modified July 10, 2007. Version 1.
Checksum: 7E254C15DF5FFF97

FASTA28732,461
        10         20         30         40         50         60 
MPDELKPHFA NVQAHYDLSD DFFRLFLDPT QTYSCAYFER DDMTLQEAQI AKIDLALGKL 

        70         80         90        100        110        120 
GLQPGMTLLD VGCGWGATMM RAVEKYDVNV VGLTLSKNQA NHVQQLVANS ENLRSKRVLL 

       130        140        150        160        170        180 
AGWEQFDEPV DRIVSIGAFE HFGHERYDAF FSLAHRLLPA DGVMLLHTIT GLHPKEIHER 

       190        200        210        220        230        240 
GLPMSFTFAR FLKFIVTEIF PGGRLPSIPM VQECASANGF TVTRVQSLQP HYAKTLDLWS 

       250        260        270        280 
AALQANKGQA IALQSEEVYE RYMKYLTGCA EMFRIGYIDV NQFTCQK 

« Hide

References

« Hide 'large scale' references
[1]"Identification of a gene involved in the biosynthesis of cyclopropanated mycolic acids in Mycobacterium tuberculosis."
Yuan Y., Lee R.E., Besra G.S., Belisle J.T., Barry C.E. III
Proc. Natl. Acad. Sci. U.S.A. 92:6630-6634(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION AS A CYCLOPROPANE SYNTHASE, NOMENCLATURE.
[2]"Complete genomic sequence of Mycobacterium tuberculosis strain H37Ra, a non-pathogenic variant closely related to the well-characterized pathogenic strain H37Rv."
Wang S.Y., Zheng H.J., Lv L.D., Wang B.F., Zhang X.L., Pu S.Y., Zhu G.F., Wang H.H., Zhao G.P., Zhang Y.
Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 25177 / H37Ra.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U27357 Genomic DNA. Translation: AAA75624.1.
CP000611 Genomic DNA. Translation: ABQ75216.1.
RefSeqYP_001284778.1. NC_009525.1.

3D structure databases

ProteinModelPortalA5U866.
SMRA5U866. Positions 3-287.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING419947.MRA_3431.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABQ75216; ABQ75216; MRA_3431.
GeneID5214660.
KEGGmra:MRA_3431.
PATRIC18147292. VBIMycTub106795_3831.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2230.
HOGENOMHOG000245191.
KOK00574.
OMAADVQAHY.
OrthoDBEOG6BGNZP.

Enzyme and pathway databases

BioCycMTUB419947:GJ8N-3541-MONOMER.
UniPathwayUPA00915.

Family and domain databases

Gene3D3.40.50.150. 1 hit.
InterProIPR003333. Mycolic_cyclopropane_synthase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamPF02353. CMAS. 1 hit.
[Graphical view]
PIRSFPIRSF003085. CMAS. 1 hit.
SUPFAMSSF53335. SSF53335. 1 hit.
ProtoNetSearch...

Entry information

Entry nameCMAS1_MYCTA
AccessionPrimary (citable) accession number: A5U866
Secondary accession number(s): Q11195
Entry history
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: July 10, 2007
Last modified: June 11, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways