A5U7Q1 (AROA_MYCTA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-phosphoshikimate 1-carboxyvinyltransferase
EC=2.5.1.19

Alternative name(s):
5-enolpyruvylshikimate-3-phosphate synthase
Short name=EPSP synthase
Short name=EPSPS

Gene names

Name:	aroA
Ordered Locus Names:	MRA_3268

Organism

Mycobacterium tuberculosis (strain ATCC 25177 / H37Ra) [Complete proteome] [HAMAP]

Taxonomic identifier

419947 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	450 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Phosphoenolpyruvate + 3-phosphoshikimate = phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate. HAMAP MF_00210
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 6/7. HAMAP MF_00210
Subunit structure	Monomer By similarity. HAMAP MF_00210
Subcellular location	Cytoplasm By similarity HAMAP MF_00210.
Sequence similarities	Belongs to the EPSP synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-phosphoshikimate 1-carboxyvinyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 450	450	3-phosphoshikimate 1-carboxyvinyltransferase HAMAP MF_00210		PRO_1000012453

Sequences

	Sequence		Length	Mass (Da)
	A5U7Q1 [UniParc]. Last modified July 10, 2007. Version 1. Checksum: 27BB86F9412A07D5	FASTA	450	46,426
	10 20 30 40 50 60 MKTWPAPTAP TPVRATVTVP GSKSQTNRAL VLAALAAAQG RGASTISGAL RSRDTELMLD 70 80 90 100 110 120 ALQTLGLRVD GVGSELTVSG RIEPGPGARV DCGLAGTVLR FVPPLAALGS VPVTFDGDQQ 130 140 150 160 170 180 ARGRPIAPLL DALRELGVAV DGTGLPFRVR GNGSLAGGTV AIDASASSQF VSGLLLSAAS 190 200 210 220 230 240 FTDGLTVQHT GSSLPSAPHI AMTAAMLRQA GVDIDDSTPN RWQVRPGPVA ARRWDIEPDL 250 260 270 280 290 300 TNAVAFLSAA VVSGGTVRIT GWPRVSVQPA DHILAILRQL NAVVIHADSS LEVRGPTGYD 310 320 330 340 350 360 GFDVDLRAVG ELTPSVAALA ALASPGSVSR LSGIAHLRGH ETDRLAALST EINRLGGTCR 370 380 390 400 410 420 ETPDGLVITA TPLRPGIWRA YADHRMAMAG AIIGLRVAGV EVDDIAATTK TLPEFPRLWA 430 440 450 EMVGPGQGWG YPQPRSGQRA RRATGQGSGG

References

[1]

"Complete genomic sequence of Mycobacterium tuberculosis strain H37Ra, a non-pathogenic variant closely related to the well-characterized pathogenic strain H37Rv." Wang S.Y., Zheng H.J., Lv L.D., Wang B.F., Zhang X.L., Pu S.Y., Zhu G.F., Wang H.H., Zhao G.P., Zhang Y. Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25177 / H37Ra.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000611 Genomic DNA. Translation: ABQ75051.1.
RefSeq	YP_001284613.1. NC_009525.1.
3D structure databases
ProteinModelPortal	A5U7Q1.
SMR	A5U7Q1. Positions 1-424.
ModBase	Search...
Protein-protein interaction databases
STRING	A5U7Q1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000066432; EBMYCP00000064542; EBMYCG00000066427.
GeneID	5214238.
GenomeReviews	Gene locus MRA_3268 in contig CP000611_GR.
KEGG	mra:MRA_3268.
PATRIC	18146937. VBIMycTub106795_3654.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0128.
GeneTree	EBGT00050000016231.
HOGENOM	HBG646626.
OMA	HRMATAG.
ProtClustDB	PRK02427.
Enzyme and pathway databases
BioCyc	MTUB419947:MRA_3268-MONOMER.
Family and domain databases
HAMAP	MF_00210. EPSP_synth. [Tree]
InterPro	IPR001986. Enolpyruvate_Tfrase_dom. IPR006264. EPSP_synthase. IPR023193. EPSP_synthase_CS. IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b. [Graphical view]
Gene3D	G3DSA:3.65.10.10. EPSP_synthase. 2 hits.
KO	K00800.
Pfam	PF00275. EPSP_synthase. 1 hit. [Graphical view]
PIRSF	PIRSF000505. EPSPS. 1 hit.
SUPFAM	SSF55205. RNA3'_cycl/enolpyr_transf_A/B. 1 hit.
TIGRFAMs	TIGR01356. AroA. 1 hit.
PROSITE	PS00104. EPSP_SYNTHASE_1. 1 hit. PS00885. EPSP_SYNTHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROA_MYCTA

Accession

Primary (citable) accession number: A5U7Q1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	July 10, 2007
Last modified:	December 14, 2011
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program