A5U713 (ILVC_MYCTA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ketol-acid reductoisomerase
EC=1.1.1.86

Alternative name(s):
Acetohydroxy-acid isomeroreductase
Alpha-keto-beta-hydroxylacil reductoisomerase

Gene names

Name:	ilvC
Ordered Locus Names:	MRA_3031

Organism

Mycobacterium tuberculosis (strain ATCC 25177 / H37Ra) [Complete proteome] [HAMAP]

Taxonomic identifier

419947 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	333 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(R)-2,3-dihydroxy-3-methylbutanoate + NADP+ = (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH. HAMAP MF_00435 (2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP+ = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH. HAMAP MF_00435
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4. HAMAP MF_00435 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4. HAMAP MF_00435
Sequence similarities	Belongs to the ketol-acid reductoisomerase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	coenzyme binding Inferred from electronic annotation. Source: InterPro ketol-acid reductoisomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 333	333	Ketol-acid reductoisomerase HAMAP MF_00435		PRO_1000050543
Sites
	Active site	105	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	A5U713 [UniParc]. Last modified July 10, 2007. Version 1. Checksum: 49669E3EDACE25EC	FASTA	333	36,090
	10 20 30 40 50 60 MFYDDDADLS IIQGRKVGVI GYGSQGHAHS LSLRDSGVQV RVGLKQGSRS RPKVEEQGLD 70 80 90 100 110 120 VDTPAEVAKW ADVVMVLAPD TAQAEIFAGD IEPNLKPGDA LFFGHGLNVH FGLIKPPADV 130 140 150 160 170 180 AVAMVAPKGP GHLVRRQFVD GKGVPCLVAV EQDPRGDGLA LALSYAKAIG GTRAGVIKTT 190 200 210 220 230 240 FKDETETDLF GEQTVLCGGT EELVKAGFEV MVEAGYPAEL AYFEVLHELK LIVDLMYEGG 250 260 270 280 290 300 LARMYYSVSD TAEFGGYLSG PRVIDAGTKE RMRDILREIQ DGSFVHKLVA DVEGGNKQLE 310 320 330 ELRRQNAEHP IEVVGKKLRD LMSWVDRPIT ETA

References

[1]

"Complete genomic sequence of Mycobacterium tuberculosis strain H37Ra, a non-pathogenic variant closely related to the well-characterized pathogenic strain H37Rv." Wang S.Y., Zheng H.J., Lv L.D., Wang B.F., Zhang X.L., Pu S.Y., Zhu G.F., Wang H.H., Zhao G.P., Zhang Y. Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25177 / H37Ra.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000611 Genomic DNA. Translation: ABQ74813.1.
RefSeq	YP_001284375.1. NC_009525.1.
3D structure databases
ProteinModelPortal	A5U713.
SMR	A5U713. Positions 1-325.
ModBase	Search...
Protein-protein interaction databases
STRING	A5U713.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000067119; EBMYCP00000065229; EBMYCG00000067114.
GeneID	5215322.
GenomeReviews	Gene locus MRA_3031 in contig CP000611_GR.
KEGG	mra:MRA_3031.
PATRIC	18146417. VBIMycTub106795_3396.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0059.
GeneTree	EBGT00050000015910.
HOGENOM	HBG286525.
OMA	EYANGRG.
ProtClustDB	PRK05479.
Enzyme and pathway databases
BioCyc	MTUB419947:MRA_3031-MONOMER.
Family and domain databases
HAMAP	MF_00435. IlvC. [Tree]
InterPro	IPR008927. 6-PGluconate_DH_C-like. IPR013023. AcH_isomrdctse. IPR000506. AcH_isomrdctse_C. IPR013328. DH_multihelical. IPR013116. IlvN. [Graphical view]
Gene3D	G3DSA:1.10.1040.10. Opine_DH. 1 hit.
KO	K00053.
PANTHER	PTHR21371. AcH_isomrdctse. 1 hit.
Pfam	PF01450. IlvC. 1 hit. PF07991. IlvN. 1 hit. [Graphical view]
SUPFAM	SSF48179. 6DGDH_C_like. 1 hit.
TIGRFAMs	TIGR00465. IlvC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ILVC_MYCTA

Accession

Primary (citable) accession number: A5U713

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	July 10, 2007
Last modified:	December 14, 2011
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program