A5U6Q7 (RNH2_MYCTA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease HII
Short name=RNase HII
EC=3.1.26.4

Gene names

Name:	rnhB
Ordered Locus Names:	MRA_2927

Organism

Mycobacterium tuberculosis (strain ATCC 25177 / H37Ra) [Complete proteome] [HAMAP]

Taxonomic identifier

419947 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	264 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Endonuclease that specifically degrades the RNA of RNA-DNA hybrids By similarity. HAMAP MF_00052_B
Catalytic activity	Endonucleolytic cleavage to 5'-phosphomonoester. HAMAP MF_00052_B
Cofactor	Manganese or magnesium. Binds 1 divalent metal ion per monomer in the absence of substrate. May bind a second metal ion after substrate binding By similarity.
Subcellular location	Cytoplasm Potential HAMAP MF_00052_B.
Sequence similarities	Belongs to the RNase HII family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Manganese Metal-binding
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid binding Inferred from electronic annotation. Source: InterPro ribonuclease H activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 264	264	Ribonuclease HII HAMAP MF_00052_B		PRO_1000031168
Sites
	Metal binding	39	1	Divalent metal cation By similarity
	Metal binding	40	1	Divalent metal cation By similarity
	Metal binding	133	1	Divalent metal cation By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5U6Q7 [UniParc]. Last modified July 10, 2007. Version 1. Checksum: 786F0E790188279F	FASTA	264	27,646
	10 20 30 40 50 60 MTKTWPPRTV IRKSGGLRGM RTLESALHRG GLGPVAGVDE VGRGACAGPL VVAACVLGPG 70 80 90 100 110 120 RIASLAALDD SKKLSEQARE KLFPLICRYA VAYHVVFIPS AEVDRRGVHV ANIEGMRRAV 130 140 150 160 170 180 AGLAVRPGYV LSDGFRVPGL PMPSLPVIGG DAAAACIAAA SVLAKVSRDR VMVALDADHP 190 200 210 220 230 240 GYGFAEHKGY STPAHSRALA RLGPCPQHRY SFINVRRVAS GSNTAEVADG QPDPRDGTAQ 250 260 TGEGRWSKSS HPATMRATGR AQGT

References

[1]

"Complete genomic sequence of Mycobacterium tuberculosis strain H37Ra, a non-pathogenic variant closely related to the well-characterized pathogenic strain H37Rv." Wang S.Y., Zheng H.J., Lv L.D., Wang B.F., Zhang X.L., Pu S.Y., Zhu G.F., Wang H.H., Zhao G.P., Zhang Y. Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25177 / H37Ra.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000611 Genomic DNA. Translation: ABQ74707.1.
RefSeq	YP_001284269.1. NC_009525.1.
3D structure databases
ProteinModelPortal	A5U6Q7.
ModBase	Search...
Protein-protein interaction databases
STRING	A5U6Q7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000067644; EBMYCP00000065754; EBMYCG00000067639.
GeneID	5213704.
GenomeReviews	Gene locus MRA_2927 in contig CP000611_GR.
KEGG	mra:MRA_2927.
PATRIC	18146177. VBIMycTub106795_3277.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0164.
GeneTree	EBGT00050000016477.
HOGENOM	HBG584843.
OMA	RLGPTPI.
ProtClustDB	PRK00015.
Enzyme and pathway databases
BioCyc	MTUB419947:MRA_2927-MONOMER.
Family and domain databases
HAMAP	MF_00052_B. RNase_HII_B. [Tree]
InterPro	IPR022898. RNase_HII. IPR024567. RNase_HII/HIII_dom. IPR012337. RNaseH-like_dom. [Graphical view]
KO	K03470.
Pfam	PF01351. RNase_HII. 1 hit. [Graphical view]
SUPFAM	SSF53098. RNaseH_fold. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RNH2_MYCTA

Accession

Primary (citable) accession number: A5U6Q7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	July 10, 2007
Last modified:	December 14, 2011
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program