A5U6B7 (TYSY_MYCTA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thymidylate synthase
Short name=TS
Short name=TSase
EC=2.1.1.45

Gene names

Name:	thyA
Ordered Locus Names:	MRA_2789

Organism

Mycobacterium tuberculosis (strain ATCC 25177 / H37Ra) [Complete proteome] [HAMAP]

Taxonomic identifier

419947 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	263 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Provides the sole de novo source of dTMP for DNA biosynthesis By similarity. HAMAP MF_00008
Catalytic activity	5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP MF_00008
Pathway	Pyrimidine metabolism; dTTP biosynthesis. HAMAP MF_00008
Subunit structure	Homodimer By similarity. HAMAP MF_00008
Subcellular location	Cytoplasm By similarity HAMAP MF_00008.
Sequence similarities	Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	dTMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	thymidylate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 263	263	Thymidylate synthase HAMAP MF_00008		PRO_1000000634
Sites
	Active site	146	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5U6B7 [UniParc]. Last modified July 10, 2007. Version 1. Checksum: DF48279E3E9EA5F3	FASTA	263	29,853
	10 20 30 40 50 60 MTPYEDLLRF VLETGTPKSD RTGTGTRSLF GQQMRYDLSA GFPLLTTKKV HFKSVAYELL 70 80 90 100 110 120 WFLRGDSNIG WLHEHGVTIW DEWASDTGEL GPIYGVQWRS WPAPSGEHID QISAALDLLR 130 140 150 160 170 180 TDPDSRRIIV SAWNVGEIER MALPPCHAFF QFYVADGRLS CQLYQRSADL FLGVPFNIAS 190 200 210 220 230 240 YALLTHMMAA QAGLSVGEFI WTGGDCHIYD NHVEQVRLQL SREPRPYPKL LLADRDSIFE 250 260 YTYEDIVVKN YDPHPAIKAP VAV

References

[1]

"Complete genomic sequence of Mycobacterium tuberculosis strain H37Ra, a non-pathogenic variant closely related to the well-characterized pathogenic strain H37Rv." Wang S.Y., Zheng H.J., Lv L.D., Wang B.F., Zhang X.L., Pu S.Y., Zhu G.F., Wang H.H., Zhao G.P., Zhang Y. Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25177 / H37Ra.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000611 Genomic DNA. Translation: ABQ74567.1.
RefSeq	YP_001284129.1. NC_009525.1.
3D structure databases
ProteinModelPortal	A5U6B7.
SMR	A5U6B7. Positions 1-263.
ModBase	Search...
Protein-protein interaction databases
STRING	A5U6B7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000067039; EBMYCP00000065149; EBMYCG00000067034.
GeneID	5212318.
GenomeReviews	Gene locus MRA_2789 in contig CP000611_GR.
KEGG	mra:MRA_2789.
PATRIC	18145865. VBIMycTub106795_3121.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0207.
GeneTree	EBGT00050000017116.
HOGENOM	HBG588098.
OMA	RMALAPC.
ProtClustDB	PRK01827.
Enzyme and pathway databases
BioCyc	MTUB419947:MRA_2789-MONOMER.
Family and domain databases
HAMAP	MF_00008. Thymidy_synth_bact. [Tree]
InterPro	IPR023451. Thymidate_synth/dCMP_Mease. IPR000398. Thymidylate_synthase. IPR020940. Thymidylate_synthase_AS. [Graphical view]
Gene3D	G3DSA:3.30.572.10. Thymidylat_synth_C. 1 hit.
KO	K00560.
PANTHER	PTHR11549:SF2. Thymidylat_synth_C. 1 hit.
Pfam	PF00303. Thymidylat_synt. 1 hit. [Graphical view]
PRINTS	PR00108. THYMDSNTHASE.
SUPFAM	SSF55831. Thymidylat_synth_C. 1 hit.
TIGRFAMs	TIGR03284. Thym_sym. 2 hits.
PROSITE	PS00091. THYMIDYLATE_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TYSY_MYCTA

Accession

Primary (citable) accession number: A5U6B7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	July 10, 2007
Last modified:	December 14, 2011
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program