Skip Header

Contribute Send feedback
Read comments (?) or add your own

A5U6A6 (DAPA_MYCTA) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 33. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Dihydrodipicolinate synthase
Short name=DHDPS
EC=4.2.1.52
Gene names
Name:dapA
Ordered Locus Names:MRA_2778
OrganismMycobacterium tuberculosis (strain ATCC 25177 / H37Ra) [Complete proteome] [HAMAP]
Taxonomic identifier419947 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length300 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

L-aspartate 4-semialdehyde + pyruvate = dihydrodipicolinate + 2 H2O. HAMAP MF_00418

Pathway

Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 3/4. HAMAP MF_00418

Subunit structure

Homotetramer By similarity. HAMAP MF_00418

Subcellular location

Cytoplasm By similarity HAMAP MF_00418.

Sequence similarities

Belongs to the DHDPS family.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Diaminopimelate biosynthesis
Lysine biosynthesis
   Cellular componentCytoplasm
   LigandSchiff base
   Molecular functionLyase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processdiaminopimelate biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functiondihydrodipicolinate synthase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 300300Dihydrodipicolinate synthase HAMAP MF_00418
PRO_1000050227

Regions

Region58 – 592Pyruvate binding By similarity

Sites

Active site1711Schiff-base intermediate with substrate By similarity
Binding site1161Pyruvate By similarity
Site1431Involved in proton transfer during cleavage By similarity

Sequences

Sequence LengthMass (Da)Tools
A5U6A6 [UniParc].

Last modified July 10, 2007. Version 1.
Checksum: 71CB322661416367

FASTA30030,858
        10         20         30         40         50         60 
MTTVGFDVAA RLGTLLTAMV TPFSGDGSLD TATAARLANH LVDQGCDGLV VSGTTGESPT 

        70         80         90        100        110        120 
TTDGEKIELL RAVLEAVGDR ARVIAGAGTY DTAHSIRLAK ACAAEGAHGL LVVTPYYSKP 

       130        140        150        160        170        180 
PQRGLQAHFT AVADATELPM LLYDIPGRSA VPIEPDTIRA LASHPNIVGV KDAKADLHSG 

       190        200        210        220        230        240 
AQIMADTGLA YYSGDDALNL PWLAMGATGF ISVIAHLAAG QLRELLSAFG SGDIATARKI 

       250        260        270        280        290        300 
NIAVAPLCNA MSRLGGVTLS KAGLRLQGID VGDPRLPQVA ATPEQIDALA ADMRAASVLR

« Hide

References

[1]"Complete genomic sequence of Mycobacterium tuberculosis strain H37Ra, a non-pathogenic variant closely related to the well-characterized pathogenic strain H37Rv."
Wang S.Y., Zheng H.J., Lv L.D., Wang B.F., Zhang X.L., Pu S.Y., Zhu G.F., Wang H.H., Zhao G.P., Zhang Y.
Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 25177 / H37Ra.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000611 Genomic DNA. Translation: ABQ74556.1.
RefSeqYP_001284118.1. NC_009525.1.

3D structure databases

ProteinModelPortalA5U6A6.
SMRA5U6A6. Positions 5-300.
ModBaseSearch...

Protein-protein interaction databases

STRINGA5U6A6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBMYCT00000067226; EBMYCP00000065336; EBMYCG00000067221.
GeneID5213593.
GenomeReviewsGene locus MRA_2778 in contig CP000611_GR.
KEGGmra:MRA_2778.
PATRIC18145841. VBIMycTub106795_3109.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0329.
GeneTreeEBGT00050000016215.
HOGENOMHBG358848.
OMAHGCISVV.
ProtClustDBPRK03170.

Enzyme and pathway databases

BioCycMTUB419947:MRA_2778-MONOMER.

Family and domain databases

HAMAPMF_00418. DapA.
[Tree]
InterProIPR013785. Aldolase_TIM.
IPR002220. Dihydrodipicolinate_synth-like.
IPR020625. Dihydrodipicolinate_synth_AS.
IPR020624. Dihydrodipicolinate_synth_CS.
IPR005263. Dihydrodipicolinate_synth_DapA.
[Graphical view]
Gene3DG3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KOK01714.
PANTHERPTHR12128. DHDPS. 1 hit.
PfamPF00701. DHDPS. 1 hit.
[Graphical view]
PIRSFPIRSF001365. DHDPS. 1 hit.
PRINTSPR00146. DHPICSNTHASE.
TIGRFAMsTIGR00674. DapA. 1 hit.
PROSITEPS00665. DHDPS_1. 1 hit.
PS00666. DHDPS_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDAPA_MYCTA
AccessionPrimary (citable) accession number: A5U6A6
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: July 10, 2007
Last modified: December 14, 2011
This is version 33 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents