SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

A5U678

- DAPF_MYCTA

UniProt

A5U678 - DAPF_MYCTA

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Diaminopimelate epimerase
Gene
dapF, MRA_2753
Organism
Mycobacterium tuberculosis (strain ATCC 25177 / H37Ra)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine and an essential component of the bacterial peptidoglycan By similarity.UniRule annotation

Catalytic activityi

LL-2,6-diaminoheptanedioate = meso-diaminoheptanedioate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei11 – 111Substrate By similarity
Binding sitei44 – 441Substrate By similarity
Binding sitei78 – 781Substrate By similarity
Active sitei87 – 871Proton donor/acceptor By similarity
Binding sitei163 – 1631Substrate By similarity
Sitei165 – 1651Important for catalytic activity By similarity
Binding sitei199 – 1991Substrate By similarity
Sitei217 – 2171Important for catalytic activity By similarity
Active sitei226 – 2261Proton donor/acceptor By similarity

GO - Molecular functioni

  1. diaminopimelate epimerase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. lysine biosynthetic process via diaminopimelate Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Amino-acid biosynthesis, Lysine biosynthesis

Enzyme and pathway databases

BioCyciMTUB419947:GJ8N-2848-MONOMER.
UniPathwayiUPA00034; UER00025.

Names & Taxonomyi

Protein namesi
Recommended name:
Diaminopimelate epimerase (EC:5.1.1.7)
Short name:
DAP epimerase
Gene namesi
Name:dapF
Ordered Locus Names:MRA_2753
OrganismiMycobacterium tuberculosis (strain ATCC 25177 / H37Ra)
Taxonomic identifieri419947 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
ProteomesiUP000001988: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 289289Diaminopimelate epimeraseUniRule annotation
PRO_1000011912Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi87 ↔ 226UniRule annotation

Keywords - PTMi

Disulfide bond

Interactioni

Protein-protein interaction databases

STRINGi419947.MRA_2753.

Structurei

3D structure databases

ProteinModelPortaliA5U678.
SMRiA5U678. Positions 1-289.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni87 – 893Substrate binding By similarity
Regioni217 – 2182Substrate binding By similarity
Regioni227 – 2282Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0253.
HOGENOMiHOG000220467.
KOiK01778.
OMAiLIVEPPY.
OrthoDBiEOG6ND0M5.

Family and domain databases

HAMAPiMF_00197. DAP_epimerase.
InterProiIPR018510. DAP_epimerase_AS.
IPR001653. DAP_epimerase_DapF.
[Graphical view]
PANTHERiPTHR31689. PTHR31689. 1 hit.
PfamiPF01678. DAP_epimerase. 2 hits.
[Graphical view]
TIGRFAMsiTIGR00652. DapF. 1 hit.
PROSITEiPS01326. DAP_EPIMERASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A5U678-1 [UniParc]FASTAAdd to Basket

« Hide

MIFAKGHGTQ NDFVLLPDVD AELVLTAARV AALCDRRKGL GADGVLRVTT    50
AGAAQAVGVL DSLPEGVRVT DWYMDYRNAD GSAAQMCGNG VRVFAHYLRA 100
SGLEVRDEFV VGSLAGPRPV TCHHVEAAYA DVSVDMGKAN RLGAGEAVVG 150
GRRFHGLAVD VGNPHLACVD SQLTVDGLAA LDVGAPVSFD GAQFPDGVNV 200
EVLTAPVDGA VWMRVHERGV GETRSCGTGT VAAAVAALAA VGSPTGTLTV 250
HVPGGEVVVT VTDATSFLRG PSVLVARGDL ADDWWNAMG 289
Length:289
Mass (Da):29,698
Last modified:July 10, 2007 - v1
Checksum:i197FE4B43CD8A022
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000611 Genomic DNA. Translation: ABQ74528.1.
RefSeqiYP_001284090.1. NC_009525.1.

Genome annotation databases

EnsemblBacteriaiABQ74528; ABQ74528; MRA_2753.
GeneIDi5214173.
KEGGimra:MRA_2753.
PATRICi18145777. VBIMycTub106795_3077.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000611 Genomic DNA. Translation: ABQ74528.1 .
RefSeqi YP_001284090.1. NC_009525.1.

3D structure databases

ProteinModelPortali A5U678.
SMRi A5U678. Positions 1-289.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 419947.MRA_2753.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABQ74528 ; ABQ74528 ; MRA_2753 .
GeneIDi 5214173.
KEGGi mra:MRA_2753.
PATRICi 18145777. VBIMycTub106795_3077.

Phylogenomic databases

eggNOGi COG0253.
HOGENOMi HOG000220467.
KOi K01778.
OMAi LIVEPPY.
OrthoDBi EOG6ND0M5.

Enzyme and pathway databases

UniPathwayi UPA00034 ; UER00025 .
BioCyci MTUB419947:GJ8N-2848-MONOMER.

Family and domain databases

HAMAPi MF_00197. DAP_epimerase.
InterProi IPR018510. DAP_epimerase_AS.
IPR001653. DAP_epimerase_DapF.
[Graphical view ]
PANTHERi PTHR31689. PTHR31689. 1 hit.
Pfami PF01678. DAP_epimerase. 2 hits.
[Graphical view ]
TIGRFAMsi TIGR00652. DapF. 1 hit.
PROSITEi PS01326. DAP_EPIMERASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete genomic sequence of Mycobacterium tuberculosis strain H37Ra, a non-pathogenic variant closely related to the well-characterized pathogenic strain H37Rv."
    Wang S.Y., Zheng H.J., Lv L.D., Wang B.F., Zhang X.L., Pu S.Y., Zhu G.F., Wang H.H., Zhao G.P., Zhang Y.
    Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 25177 / H37Ra.

Entry informationi

Entry nameiDAPF_MYCTA
AccessioniPrimary (citable) accession number: A5U678
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: July 10, 2007
Last modified: June 11, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi