Reviewed, UniProtKB/Swiss-Prot A5U665 (STHA_MYCTA)

Names and origin

Protein names

Recommended name:
    Soluble pyridine nucleotide transhydrogenase
      Short name=STH
    EC=1.6.1.1
Alternative name(s):
    NAD(P)(+) transhydrogenase [B-specific]

Gene names

Name:	sthA
Ordered Locus Names:	MRA_2741

Organism

Mycobacterium tuberculosis (strain ATCC 25177 / H37Ra) [Complete proteome] [HAMAP]

Taxonomic identifier

419947 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	468 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Conversion of NADPH, generated by peripheral catabolic pathways, to NADH, which can enter the respiratory chain for energy generation By similarity.
Catalytic activity	NADPH + NAD(+) = NADP(+) + NADH.
Cofactor	Binds 1 FAD per subunit By similarity.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NADP metabolic process Inferred from electronic annotation. Source: HAMAP cell redox homeostasis Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro NAD(P)+ transhydrogenase (B-specific) activity Inferred from electronic annotation. Source: HAMAP electron carrier activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 468	468	Soluble pyridine nucleotide transhydrogenase		PRO_1000012560
Regions
	Nucleotide binding	33 – 42	10	FAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5U665-1 [UniParc]. Last modified July 10, 2007. Version 1. Checksum: D9E737C41C2898CF	FASTA	468	50,754
	10 20 30 40 50 60 MREYDIVVIG SGPGGQKAAI ASAKLGKSVA IVERGRMLGG VCVNTGTIPS KTLREAVLYL 70 80 90 100 110 120 TGMNQRELYG ASYRVKDRIT PADLLARTQH VIGKEVDVVR NQLMRNRVDL IVGHGRFIDP 130 140 150 160 170 180 HTILVEDQAR REKTTVTGDY IIIATGTRPA RPSGVEFDEE RVLDSDGILD LKSLPSSMVV 190 200 210 220 230 240 VGAGVIGIEY ASMFAALGTK VTVVEKRDNM LDFCDPEVVE ALKFHLRDLA VTFRFGEEVT 250 260 270 280 290 300 AVDVGSAGTV TTLASGKQIP AETVMYSAGR QGQTDHLDLH NAGLEVQGRG RIFVDDRFQT 310 320 330 340 350 360 KVDHIYAVGD VIGFPALAAT SMEQGRLAAY HAFGEPTDGI TELQPIGIYS IPEVSYVGAT 370 380 390 400 410 420 EVELTKSSIP YEVGVARYRE LARGQIAGDS YGMLKLLVST EDLKLLGVHI FGTSATEMVH 430 440 450 460 IGQAVMGCGG SVEYLVDAVF NYPTFSEAYK NAALDVMNKM RALNQFRR

References

[1]

"Complete genomic sequence of Mycobacterium tuberculosis strain H37Ra, a non-pathogenic variant closely related to the well-characterized pathogenic strain H37Rv." Wang S.Y., Zheng H.J., Lv L.D., Wang B.F., Zhang X.L., Pu S.Y., Zhu G.F., Wang H.H., Zhao G.P., Zhang Y. Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000611 Genomic DNA. Translation: ABQ74515.1.
RefSeq	YP_001284077.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5214606.
GenomeReviews	Gene locus MRA_2741 in contig CP000611_GR.
KEGG	mra:MRA_2741.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_00247. [Tree]
InterPro	IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR000815. Hg_reductase. IPR001100. Pyr_nuc-diS_OxRdtase. IPR004099. Pyr_nucl-diS_OxRdtase_dimer. IPR001327. Pyr_OxRdtase_NAD_bd. [Graphical view]
Gene3D	G3DSA:3.30.390.30. Pyr_redox_dim. 1 hit.
Pfam	PF00070. Pyr_redox. 1 hit. PF07992. Pyr_redox_2. 1 hit. PF02852. Pyr_redox_dim. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR. PR00945. HGRDTASE. PR00411. PNDRDTASEI.
ProDom	PD000139. FAD_pyr_redox. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

STHA_MYCTA

Accession

Primary (citable) accession number: A5U665

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	July 10, 2007
Last modified:	November 25, 2008
This is version 14 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)