Skip Header

Contribute Send feedback
Read comments (?) or add your own

A5U634 (DXS_MYCTA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1-deoxy-D-xylulose-5-phosphate synthase
EC=2.2.1.7
Alternative name(s):
1-deoxyxylulose-5-phosphate synthase
Short name=DXP synthase
Short name=DXPS
Gene names
Name:dxs
Ordered Locus Names:MRA_2710
OrganismMycobacterium tuberculosis (strain ATCC 25177 / H37Ra) [Complete proteome] [HAMAP]
Taxonomic identifier419947 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex ›

Protein attributes

Sequence length638 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP) By similarity. HAMAP-Rule MF_00315

Catalytic activity

Pyruvate + D-glyceraldehyde 3-phosphate = 1-deoxy-D-xylulose 5-phosphate + CO2. HAMAP-Rule MF_00315

Cofactor

Binds 1 magnesium ion per subunit By similarity. HAMAP-Rule MF_00315

Binds 1 thiamine pyrophosphate per subunit By similarity.

Pathway

Metabolic intermediate biosynthesis; 1-deoxy-D-xylulose 5-phosphate biosynthesis; 1-deoxy-D-xylulose 5-phosphate from D-glyceraldehyde 3-phosphate and pyruvate: step 1/1. HAMAP-Rule MF_00315

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00315

Sequence similarities

Belongs to the transketolase family. DXPS subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 6386381-deoxy-D-xylulose-5-phosphate synthase HAMAP-Rule MF_00315
PRO_1000019045

Regions

Region112 – 1143Thiamine pyrophosphate binding By similarity
Region145 – 1462Thiamine pyrophosphate binding By similarity

Sites

Metal binding1441Magnesium By similarity
Metal binding1731Magnesium By similarity
Binding site711Thiamine pyrophosphate By similarity
Binding site1731Thiamine pyrophosphate By similarity
Binding site2841Thiamine pyrophosphate By similarity
Binding site3651Thiamine pyrophosphate By similarity

Sequences

Sequence LengthMass (Da)Tools
A5U634 [UniParc].

Last modified July 10, 2007. Version 1.
Checksum: 6F8DC2A78290873F

FASTA63867,885
        10         20         30         40         50         60 
MLQQIRGPAD LQHLSQAQLR ELAAEIREFL IHKVAATGGH LGPNLGVVEL TLALHRVFDS 

        70         80         90        100        110        120 
PHDPIIFDTG HQAYVHKMLT GRSQDFATLR KKGGLSGYPS RAESEHDWVE SSHASAALSY 

       130        140        150        160        170        180 
ADGLAKAFEL TGHRNRHVVA VVGDGALTGG MCWEALNNIA ASRRPVIIVV NDNGRSYAPT 

       190        200        210        220        230        240 
IGGVADHLAT LRLQPAYEQA LETGRDLVRA VPLVGGLWFR FLHSVKAGIK DSLSPQLLFT 

       250        260        270        280        290        300 
DLGLKYVGPV DGHDERAVEV ALRSARRFGA PVIVHVVTRK GMGYPPAEAD QAEQMHSTVP 

       310        320        330        340        350        360 
IDPATGQATK VAGPGWTATF SDALIGYAQK RRDIVAITAA MPGPTGLTAF GQRFPDRLFD 

       370        380        390        400        410        420 
VGIAEQHAMT SAAGLAMGGL HPVVAIYSTF LNRAFDQIMM DVALHKLPVT MVLDRAGITG 

       430        440        450        460        470        480 
SDGASHNGMW DLSMLGIVPG IRVAAPRDAT RLREELGEAL DVDDGPTALR FPKGDVGEDI 

       490        500        510        520        530        540 
SALERRGGVD VLAAPADGLN HDVLLVAIGA FAPMALAVAK RLHNQGIGVT VIDPRWVLPV 

       550        560        570        580        590        600 
SDGVRELAVQ HKLLVTLEDN GVNGGAGSAV SAALRRAEID VPCRDVGLPQ EFYEHASRSE 

       610        620        630 
VLADLGLTDQ DVARRITGWV AALGTGVCAS DAIPEHLD

« Hide

References

[1]"Complete genomic sequence of Mycobacterium tuberculosis strain H37Ra, a non-pathogenic variant closely related to the well-characterized pathogenic strain H37Rv."
Wang S.Y., Zheng H.J., Lv L.D., Wang B.F., Zhang X.L., Pu S.Y., Zhu G.F., Wang H.H., Zhao G.P., Zhang Y.
Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 25177 / H37Ra.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000611 Genomic DNA. Translation: ABQ74484.1.
RefSeqYP_001284046.1. NC_009525.1.

3D structure databases

ProteinModelPortalA5U634.
SMRA5U634. Positions 1-607.
ModBaseSearch...

Protein-protein interaction databases

STRING419947.MRA_2710.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABQ74484; ABQ74484; MRA_2710.
GeneID5213834.
KEGGmra:MRA_2710.
PATRIC18145683. VBIMycTub106795_3030.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1154.
HOGENOMHOG000012986.
KOK01662.
OMAPVAYHGP.
ProtClustDBPRK05444.

Enzyme and pathway databases

BioCycMTUB419947:GJ8N-2804-MONOMER.
UniPathwayUPA00064; UER00091.

Family and domain databases

Gene3D3.40.50.920. 1 hit.
HAMAPMF_00315. DXP_synth.
InterProIPR005477. Dxylulose-5-P_synthase.
IPR009014. Transketo_C/Pyr-ferredox_oxred.
IPR015941. Transketolase-like_C.
IPR005475. Transketolase-like_Pyr-bd.
IPR020826. Transketolase_BS.
IPR005476. Transketolase_C.
IPR005474. Transketolase_N.
[Graphical view]
PfamPF13292. DXP_synthase_N. 1 hit.
PF02779. Transket_pyr. 1 hit.
PF02780. Transketolase_C. 1 hit.
[Graphical view]
SMARTSM00861. Transket_pyr. 1 hit.
[Graphical view]
SUPFAMSSF52922. Transketo_C_like. 1 hit.
TIGRFAMsTIGR00204. dxs. 1 hit.
PROSITEPS00801. TRANSKETOLASE_1. 1 hit.
PS00802. TRANSKETOLASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

BindingDBA5U634.

Entry information

Entry nameDXS_MYCTA
AccessionPrimary (citable) accession number: A5U634
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: July 10, 2007
Last modified: May 1, 2013
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents