A5U586 (CYSH_MYCTA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoadenosine phosphosulfate reductase
EC=1.8.4.8

Alternative name(s):
3'-phosphoadenylylsulfate reductase
PAPS reductase, thioredoxin dependent
PAPS sulfotransferase
PAdoPS reductase

Gene names

Name:	cysH
Ordered Locus Names:	MRA_2416

Organism

Mycobacterium tuberculosis (strain ATCC 25177 / H37Ra) [Complete proteome] [HAMAP]

Taxonomic identifier

419947 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	254 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Reduction of activated sulfate into sulfite. HAMAP MF_00063
Catalytic activity	Adenosine 3',5'-bisphosphate + sulfite + thioredoxin disulfide = 3'-phosphoadenylyl sulfate + thioredoxin. HAMAP MF_00063
Pathway	Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 3/3. HAMAP MF_00063
Subcellular location	Cytoplasm By similarity HAMAP MF_00063.
Sequence similarities	Belongs to the PAPS reductase family. CysH subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteine biosynthetic process Inferred from electronic annotation. Source: InterPro sulfate assimilation, phosphoadenylyl sulfate reduction by phosphoadenylyl-sulfate reductase (thioredoxin) Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	phosphoadenylyl-sulfate reductase (thioredoxin) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 254	254	Phosphoadenosine phosphosulfate reductase HAMAP MF_00063		PRO_1000008930

Sequences

	Sequence		Length	Mass (Da)
	A5U586 [UniParc]. Last modified July 10, 2007. Version 1. Checksum: 554F2E3204452C55	FASTA	254	27,423
	10 20 30 40 50 60 MSGETTRLTE PQLRELAARG AAELDGATAT DMLRWTDETF GDIGGAGGGV SGHRGWTTCN 70 80 90 100 110 120 YVVASNMADA VLVDLAAKVR PGVPVIFLDT GYHFVETIGT RDAIESVYDV RVLNVTPEHT 130 140 150 160 170 180 VAEQDELLGK DLFARNPHEC CRLRKVVPLG KTLRGYSAWV TGLRRVDAPT RANAPLVSFD 190 200 210 220 230 240 ETFKLVKVNP LAAWTDQDVQ EYIADNDVLV NPLVREGYPS IGCAPCTAKP AEGADPRSGR 250 WQGLAKTECG LHAS

References

[1]

"Complete genomic sequence of Mycobacterium tuberculosis strain H37Ra, a non-pathogenic variant closely related to the well-characterized pathogenic strain H37Rv." Wang S.Y., Zheng H.J., Lv L.D., Wang B.F., Zhang X.L., Pu S.Y., Zhu G.F., Wang H.H., Zhao G.P., Zhang Y. Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25177 / H37Ra.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000611 Genomic DNA. Translation: ABQ74186.1.
RefSeq	YP_001283748.1. NC_009525.1.
3D structure databases
ProteinModelPortal	A5U586.
ModBase	Search...
Protein-protein interaction databases
STRING	A5U586.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000065091; EBMYCP00000063201; EBMYCG00000065086.
GeneID	5213172.
GenomeReviews	Gene locus MRA_2416 in contig CP000611_GR.
KEGG	mra:MRA_2416.
PATRIC	18145032. VBIMycTub106795_2714.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0175.
GeneTree	EBGT00050000017137.
HOGENOM	HBG758022.
OMA	FEETLAY.
ProtClustDB	PRK02090.
Enzyme and pathway databases
BioCyc	MTUB419947:MRA_2416-MONOMER.
Family and domain databases
HAMAP	MF_00063. CysH. [Tree]
InterPro	IPR011798. APS_reductase. IPR004511. PAPS/APS_Rdtase. IPR002500. PAPS_reduct. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K00390.
Pfam	PF01507. PAPS_reduct. 1 hit. [Graphical view]
PIRSF	PIRSF000857. PAPS_reductase. 1 hit.
TIGRFAMs	TIGR02055. APS_reductase. 1 hit. TIGR00434. CysH. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CYSH_MYCTA

Accession

Primary (citable) accession number: A5U586

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	July 10, 2007
Last modified:	January 25, 2012
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program