Reviewed, UniProtKB/Swiss-Prot A5U4N0 (ADOK_MYCTA)

Names and origin

Protein names

Recommended name:
    Adenosine kinase
      Short name=AK
    EC=2.7.1.20

Gene names

Name:	adoK
Synonyms:	cbhK
Ordered Locus Names:	MRA_2218

Organism

Mycobacterium tuberculosis (strain ATCC 25177 / H37Ra) [Complete proteome] [HAMAP]

Taxonomic identifier

419947 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	324 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	ATP dependent phosphorylation of adenosine to monophosphate derivatives. The enzyme exculusively uses adenosine or methyl-adenosine, and prefers GTP and dGTP to ATP. Ref.2
Catalytic activity	ATP + adenosine = ADP + AMP.
Cofactor	Magnesium. Ref.2
Enzyme regulation	The enzyme is subject to inhibition by adenosine and is competitively inhibited by the adenosine analog idotubercidin. Unlike other adenosine kinases it is not stimulated by inorganic phosphate. Ref.2
Pathway	Purine metabolism; AMP biosynthesis via salvage pathway; AMP from adenosine 3',5'-bisphosphate: step 1/1.
Subunit structure	Homodimer Probable. Ref.2
Sequence similarities	Belongs to the carbohydrate kinase pfkB family.
Biophysicochemical properties	Kinetic parameters: Activity is stimulated in the presence of potassium, resulting in a 13-fold increase in the catalytic efficiency for adenosine. KM=0.80 µM for adenosine (in the presence of 10 mM KCl) KM=79 µM for methyl-adenosine (in the presence of 10 mM KCl) KM=1100 µM for ATP (in the presence of 10 mM KCl) Vmax=180 nmol/min/mg enzyme (for adenosine in the presence of 10 mM KCl) Vmax=72 nmol/min/mg enzyme (for methyl-adenosine in the presence of 10 mM KCl) pH dependence: Optimum pH is 8-11.
Mass spectrometry	Molecular mass is 34337 Da from positions 2 - 324. Determined by ESI. Ref.2

Ontologies

Keywords
Biological process	Purine salvage
Ligand	ATP-binding Magnesium Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	purine ribonucleoside salvage Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW adenosine kinase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.2
	Chain	2 – 324	323	Adenosine kinase		PRO_0000306417
Sites
	Active site	257	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5U4N0-1 [UniParc]. Last modified July 10, 2007. Version 1. Checksum: 0C072206A3210A1D	FASTA	324	34,472
	10 20 30 40 50 60 MTIAVTGSIA TDHLMRFPGR FSEQLLPEHL HKVSLSFLVD DLVMHRGGVA GNMAFAIGVL 70 80 90 100 110 120 GGEVALVGAA GADFADYRDW LKARGVNCDH VLISETAHTA RFTCTTDVDM AQIASFYPGA 130 140 150 160 170 180 MSEARNIKLA DVVSAIGKPE LVIIGANDPE AMFLHTEECR KLGLAFAADP SQQLARLSGE 190 200 210 220 230 240 EIRRLVNGAA YLFTNDYEWD LLLSKTGWSE ADVMAQIDLR VTTLGPKGVD LVEPDGTTIH 250 260 270 280 290 300 VGVVPETSQT DPTGVGDAFR AGFLTGRSAG LGLERSAQLG SLVAVLVLES TGTQEWQWDY 310 320 EAAASRLAGA YGEHAAAEIV AVLA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genomic sequence of Mycobacterium tuberculosis strain H37Ra, a non-pathogenic variant closely related to the well-characterized pathogenic strain H37Rv." Wang S.Y., Zheng H.J., Lv L.D., Wang B.F., Zhang X.L., Pu S.Y., Zhu G.F., Wang H.H., Zhao G.P., Zhang Y. Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"Identification and characterization of a unique adenosine kinase from Mycobacterium tuberculosis." Long M.C., Escuyer V., Parker W.B. J. Bacteriol. 185:6548-6555(2003) [PubMed: 14594827] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-16, FUNCTION, COFACTOR, SUBUNIT, ENZYME REGULATION, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000611 Genomic DNA. Translation: ABQ73980.1.
RefSeq	YP_001283542.1.
3D structure databases
SMR	A5U4N0. Positions 1-324.
ModBase	Search...
Protein-protein interaction databases
STRING	A5U4N0.
Genome annotation databases
GeneID	5214750.
GenomeReviews	Gene locus MRA_2218 in contig CP000611_GR.
KEGG	mra:MRA_2218.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0524.
HOGENOM	HBG296008.
OMA	RDNNQIT.
Family and domain databases
InterPro	IPR011611. Carb/pur_kinase. IPR002173. Carboh/pur_kinase_PfkB_CS. [Graphical view]
Pfam	PF00294. PfkB. 1 hit. [Graphical view]
PROSITE	PS00583. PFKB_KINASES_1. 1 hit. PS00584. PFKB_KINASES_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ADOK_MYCTA

Accession

Primary (citable) accession number: A5U4N0

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 2, 2007
Last sequence update:	July 10, 2007
Last modified:	January 19, 2010
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)