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A5U493

- BLAC_MYCTA

UniProt

A5U493 - BLAC_MYCTA

Protein

Beta-lactamase

Gene

blaA

Organism
Mycobacterium tuberculosis (strain ATCC 25177 / H37Ra)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 50 (01 Oct 2014)
      Sequence version 1 (10 Jul 2007)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    A beta-lactam + H2O = a substituted beta-amino acid.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei84 – 841Acyl-ester intermediatePROSITE-ProRule annotation

    GO - Molecular functioni

    1. beta-lactamase activity Source: UniProtKB-EC

    GO - Biological processi

    1. beta-lactam antibiotic catabolic process Source: InterPro
    2. response to antibiotic Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Antibiotic resistance

    Enzyme and pathway databases

    BioCyciMTUB419947:GJ8N-2149-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-lactamase (EC:3.5.2.6)
    Alternative name(s):
    Penicillinase
    Gene namesi
    Name:blaA
    Synonyms:blaC
    Ordered Locus Names:MRA_2082
    OrganismiMycobacterium tuberculosis (strain ATCC 25177 / H37Ra)
    Taxonomic identifieri419947 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
    ProteomesiUP000001988: Chromosome

    Subcellular locationi

    Cell membrane Curated; Lipid-anchor Curated

    GO - Cellular componenti

    1. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2323Sequence AnalysisAdd
    BLAST
    Chaini24 – 307284Beta-lactamasePRO_0000300061Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi24 – 241N-palmitoyl cysteineSequence Analysis
    Lipidationi24 – 241S-diacylglycerol cysteineSequence Analysis

    Keywords - PTMi

    Lipoprotein, Palmitate

    Interactioni

    Protein-protein interaction databases

    STRINGi419947.MRA_2082.

    Structurei

    Secondary structure

    1
    307
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi44 – 5411
    Beta strandi58 – 636
    Beta strandi72 – 754
    Helixi83 – 864
    Helixi87 – 9711
    Helixi100 – 1045
    Helixi111 – 1133
    Helixi121 – 1233
    Turni125 – 1273
    Helixi131 – 14010
    Helixi144 – 15411
    Helixi158 – 1603
    Helixi161 – 17010
    Helixi184 – 1863
    Helixi199 – 21012
    Beta strandi212 – 2154
    Helixi217 – 22812
    Turni234 – 2363
    Helixi237 – 2404
    Beta strandi245 – 25410
    Turni255 – 2573
    Beta strandi258 – 2669
    Beta strandi272 – 2809
    Helixi282 – 2843
    Helixi292 – 30615

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3DWZX-ray1.80A43-307[»]
    ProteinModelPortaliA5U493.
    SMRiA5U493. Positions 43-307.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiA5U493.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni250 – 2523Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the class-A beta-lactamase family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG2367.
    HOGENOMiHOG000201073.
    KOiK17836.
    OMAiMKLIGGP.
    OrthoDBiEOG6K9QFS.

    Family and domain databases

    Gene3Di3.40.710.10. 1 hit.
    InterProiIPR012338. Beta-lactam/transpept-like.
    IPR000871. Beta-lactam_class-A/D.
    IPR023650. Beta-lactam_class-A_AS.
    [Graphical view]
    PRINTSiPR00118. BLACTAMASEA.
    SUPFAMiSSF56601. SSF56601. 1 hit.
    PROSITEiPS00146. BETA_LACTAMASE_A. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    A5U493-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRNRGFGRRE LLVAMAMLVS VTGCARHASG ARPASTTLPA GADLADRFAE    50
    LERRYDARLG VYVPATGTTA AIEYRADERF AFCSTFKAPL VAAVLHQNPL 100
    THLDKLITYT SDDIRSISPV AQQHVQTGMT IGQLCDAAIR YSDGTAANLL 150
    LADLGGPGGG TAAFTGYLRS LGDTVSRLDA EEPELNRDPP GDERDTTTPH 200
    AIALVLQQLV LGNALPPDKR ALLTDWMARN TTGAKRIRAG FPADWKVIDK 250
    TGTGDYGRAN DIAVVWSPTG VPYVVAVMSD RAGGGYDAEP REALLAEAAT 300
    CVAGVLA 307
    Length:307
    Mass (Da):32,568
    Last modified:July 10, 2007 - v1
    Checksum:i448CB2A0E05F4315
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U67924 Genomic DNA. Translation: AAB07556.1.
    CP000611 Genomic DNA. Translation: ABQ73843.1.
    RefSeqiYP_001283405.1. NC_009525.1.

    Genome annotation databases

    EnsemblBacteriaiABQ73843; ABQ73843; MRA_2082.
    GeneIDi5212563.
    KEGGimra:MRA_2082.
    PATRICi18144269. VBIMycTub106795_2337.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U67924 Genomic DNA. Translation: AAB07556.1 .
    CP000611 Genomic DNA. Translation: ABQ73843.1 .
    RefSeqi YP_001283405.1. NC_009525.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3DWZ X-ray 1.80 A 43-307 [» ]
    ProteinModelPortali A5U493.
    SMRi A5U493. Positions 43-307.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 419947.MRA_2082.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABQ73843 ; ABQ73843 ; MRA_2082 .
    GeneIDi 5212563.
    KEGGi mra:MRA_2082.
    PATRICi 18144269. VBIMycTub106795_2337.

    Phylogenomic databases

    eggNOGi COG2367.
    HOGENOMi HOG000201073.
    KOi K17836.
    OMAi MKLIGGP.
    OrthoDBi EOG6K9QFS.

    Enzyme and pathway databases

    BioCyci MTUB419947:GJ8N-2149-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei A5U493.

    Family and domain databases

    Gene3Di 3.40.710.10. 1 hit.
    InterProi IPR012338. Beta-lactam/transpept-like.
    IPR000871. Beta-lactam_class-A/D.
    IPR023650. Beta-lactam_class-A_AS.
    [Graphical view ]
    PRINTSi PR00118. BLACTAMASEA.
    SUPFAMi SSF56601. SSF56601. 1 hit.
    PROSITEi PS00146. BETA_LACTAMASE_A. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and sequence analysis of a class A beta-lactamase from Mycobacterium tuberculosis H37Ra."
      Hackbarth C.J., Unsal I., Chambers H.F.
      Antimicrob. Agents Chemother. 41:1182-1185(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Complete genomic sequence of Mycobacterium tuberculosis strain H37Ra, a non-pathogenic variant closely related to the well-characterized pathogenic strain H37Rv."
      Wang S.Y., Zheng H.J., Lv L.D., Wang B.F., Zhang X.L., Pu S.Y., Zhu G.F., Wang H.H., Zhao G.P., Zhang Y.
      Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 25177 / H37Ra.

    Entry informationi

    Entry nameiBLAC_MYCTA
    AccessioniPrimary (citable) accession number: A5U493
    Secondary accession number(s): P0A5I6, Q10670
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 11, 2007
    Last sequence update: July 10, 2007
    Last modified: October 1, 2014
    This is version 50 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3