A5U493 (BLAC_MYCTA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-lactamase
EC=3.5.2.6

Alternative name(s):
Penicillinase

Gene names

Name:	blaA
Synonyms:	blaC
Ordered Locus Names:	MRA_2082

Organism

Mycobacterium tuberculosis (strain ATCC 25177 / H37Ra) [Complete proteome] [HAMAP]

Taxonomic identifier

419947 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	307 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	A beta-lactam + H2O = a substituted beta-amino acid.
Subcellular location	Cell membrane; Lipid-anchor Potential.
Sequence similarities	Belongs to the class-A beta-lactamase family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Cellular component	Cell membrane Membrane
Domain	Signal
Molecular function	Hydrolase
PTM	Lipoprotein Palmitate
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	beta-lactam antibiotic catabolic process Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	beta-lactamase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 23

23

Potential

Chain

24 – 307

284

Beta-lactamase

PRO_0000300061

Regions

Region

250 – 252

3

Substrate binding By similarity

Sites

Active site

84

1

Acyl-ester intermediate By similarity

Amino acid modifications

Lipidation

24

1

N-palmitoyl cysteine Potential

Lipidation

24

1

S-diacylglycerol cysteine Potential

Secondary structure

1

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307

Helix Strand Turn

Details...

Helix

44 – 54

11

Beta strand

72 – 75

4

Helix

83 – 86

4

Helix

87 – 97

11

Helix

100 – 104

5

Helix

111 – 113

3

Helix

121 – 123

3

Helix

134 – 140

7

Helix

146 – 153

8

Turn

162 – 164

3

Turn

168 – 170

3

Helix

184 – 187

4

Helix

199 – 210

12

Helix

217 – 228

12

Beta strand

245 – 250

6

Beta strand

258 – 266

9

Beta strand

272 – 280

9

Helix

292 – 302

11

Sequences

	Sequence		Length	Mass (Da)
	A5U493 [UniParc]. Last modified July 10, 2007. Version 1. Checksum: 448CB2A0E05F4315	FASTA	307	32,568
	10 20 30 40 50 60 MRNRGFGRRE LLVAMAMLVS VTGCARHASG ARPASTTLPA GADLADRFAE LERRYDARLG 70 80 90 100 110 120 VYVPATGTTA AIEYRADERF AFCSTFKAPL VAAVLHQNPL THLDKLITYT SDDIRSISPV 130 140 150 160 170 180 AQQHVQTGMT IGQLCDAAIR YSDGTAANLL LADLGGPGGG TAAFTGYLRS LGDTVSRLDA 190 200 210 220 230 240 EEPELNRDPP GDERDTTTPH AIALVLQQLV LGNALPPDKR ALLTDWMARN TTGAKRIRAG 250 260 270 280 290 300 FPADWKVIDK TGTGDYGRAN DIAVVWSPTG VPYVVAVMSD RAGGGYDAEP REALLAEAAT CVAGVLA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and sequence analysis of a class A beta-lactamase from Mycobacterium tuberculosis H37Ra." Hackbarth C.J., Unsal I., Chambers H.F. Antimicrob. Agents Chemother. 41:1182-1185(1997) [PubMed: 9145897] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete genomic sequence of Mycobacterium tuberculosis strain H37Ra, a non-pathogenic variant closely related to the well-characterized pathogenic strain H37Rv." Wang S.Y., Zheng H.J., Lv L.D., Wang B.F., Zhang X.L., Pu S.Y., Zhu G.F., Wang H.H., Zhao G.P., Zhang Y. Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25177 / H37Ra.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U67924 Genomic DNA. Translation: AAB07556.1.
CP000611 Genomic DNA. Translation: ABQ73843.1.

RefSeq

YP_001283405.1. NC_009525.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3DWZ	X-ray	1.80	A	43-307	[»]

ProteinModelPortal

A5U493.

SMR

A5U493. Positions 43-307.

ModBase

Search...

Protein-protein interaction databases

STRING

A5U493.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBMYCT00000068410; EBMYCP00000066520; EBMYCG00000068405.

GeneID

5212563.

GenomeReviews

Gene locus MRA_2082 in contig CP000611_GR.

KEGG

mra:MRA_2082.

PATRIC

18144269. VBIMycTub106795_2337.

Organism-specific databases

CMR

Search...

Phylogenomic databases

eggNOG

COG2367.

GeneTree

EBGT00050000016522.

HOGENOM

HBG535713.

OMA

EYGRAND.

ProtClustDB

CLSK791603.

Enzyme and pathway databases

BioCyc

MTUB419947:MRA_2082-MONOMER.

Family and domain databases

InterPro

IPR001466. Beta-lactam-related.
IPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A/D.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view]

Gene3D

G3DSA:3.40.710.10. G3DSA:3.40.710.10. 1 hit.

KO

K01467.

Pfam

PF00144. Beta-lactamase. 1 hit.
[Graphical view]

PRINTS

PR00118. BLACTAMASEA.

SUPFAM

SSF56601. PBP_transp_fold. 1 hit.

PROSITE

PS00146. BETA_LACTAMASE_A. 1 hit.
PS51257. PROKAR_LIPOPROTEIN. False negative.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

BLAC_MYCTA

Accession

Primary (citable) accession number: A5U493
Secondary accession number(s): P0A5I6, Q10670

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	July 10, 2007
Last modified:	December 14, 2011
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program