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Protein

Beta-lactamase

Gene

blaC

Organism
Mycobacterium tuberculosis (strain ATCC 25177 / H37Ra)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Extended spectrum beta-lactamase (ESBL) that inactivates beta-lactam antibiotics by hydrolyzing the amide group of the beta-lactam ring. Exhibits predominant penicillinase activity. Also displays high levels of cephalosporinase activity as well as measurable activity with carbapenems, including imipenem and meropenem. Plays a primary role in the intrinsic resistance of M.tuberculosis to beta-lactam antibiotics.By similarity1 Publication

Catalytic activityi

A beta-lactam + H2O = a substituted beta-amino acid.1 Publication

Enzyme regulationi

Is inhibited by clavulanate, sulbactam and m-aminophenylboronate but not by EDTA.1 Publication

Kineticsi

kcat is 21 sec(-1) with benzylpenicillin as substrate. kcat is 20 sec(-1) with phenoxymethylpenicillin as substrate. kcat is 2 sec(-1) with amoxicillin as substrate. kcat is 55 sec(-1) with azlocillin as substrate. kcat is 31 sec(-1) with nitrocefin as substrate. kcat is 8 sec(-1) with cephaloridine as substrate. kcat is 8 sec(-1) with cefazolin as substrate. kcat is 8 sec(-1) with cephalothin as substrate. kcat is 3 sec(-1) with cephapirin as substrate. kcat is 22 sec(-1) with cefamandole as substrate. Assays performed at pH 6.0.1 Publication

Manual assertion based on experiment ini

  1. KM=50 µM for benzylpenicillin (at pH 6.0)1 Publication
  2. KM=38 µM for phenoxymethylpenicillin (at pH 6.0)1 Publication
  3. KM=94 µM for amoxicillin (at pH 6.0)1 Publication
  4. KM=185 µM for azlocillin (at pH 6.0)1 Publication
  5. KM=81 µM for nitrocefin (at pH 6.0)1 Publication
  6. KM=798 µM for cephaloridine (at pH 6.0)1 Publication
  7. KM=490 µM for cefazolin (at pH 6.0)1 Publication
  8. KM=308 µM for cephalothin (at pH 6.0)1 Publication
  9. KM=680 µM for cephapirin (at pH 6.0)1 Publication
  10. KM=645 µM for cefamandole (at pH 6.0)1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei84Acyl-ester intermediateBy similarity1
    Sitei87Increases nucleophilicity of active site SerBy similarity1
    Sitei117Functions as a gatekeeper residue that regulates substrate accessibility to the enzyme active siteBy similarity1
    Binding sitei142SubstrateBy similarity1
    Active sitei182Proton acceptorBy similarity1

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Antibiotic resistance

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-lactamase1 Publication (EC:3.5.2.61 Publication)
    Alternative name(s):
    Ambler class A beta-lactamase1 Publication
    Gene namesi
    Name:blaC
    Synonyms:blaA1 Publication
    Ordered Locus Names:MRA_2082
    OrganismiMycobacterium tuberculosis (strain ATCC 25177 / H37Ra)
    Taxonomic identifieri419947 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
    Proteomesi
    • UP000001988 Componenti: Chromosome

    Subcellular locationi

    • Periplasm By similarity
    • Secreted 1 Publication

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Periplasm, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Signal peptidei1 – 34Tat-type signalPROSITE-ProRule annotationAdd BLAST34
    ChainiPRO_000030006135 – 307Beta-lactamaseAdd BLAST273

    Post-translational modificationi

    Exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.By similarity

    Proteomic databases

    PRIDEiA5U493.

    Interactioni

    Subunit structurei

    Monomer.By similarity

    Protein-protein interaction databases

    STRINGi419947.MtubH3_010100007057.

    Structurei

    3D structure databases

    ProteinModelPortaliA5U493.
    SMRiA5U493.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiA5U493.

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni251 – 253Substrate bindingBy similarity3

    Sequence similaritiesi

    Belongs to the class-A beta-lactamase family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiENOG4108J4B. Bacteria.
    COG2367. LUCA.
    HOGENOMiHOG000201073.
    KOiK17836.
    OMAiDEKEATY.

    Family and domain databases

    Gene3Di3.40.710.10. 1 hit.
    InterProiIPR012338. Beta-lactam/transpept-like.
    IPR000871. Beta-lactam_class-A.
    IPR023650. Beta-lactam_class-A_AS.
    [Graphical view]
    PRINTSiPR00118. BLACTAMASEA.
    SUPFAMiSSF56601. SSF56601. 1 hit.
    PROSITEiPS00146. BETA_LACTAMASE_A. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    A5U493-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MRNRGFGRRE LLVAMAMLVS VTGCARHASG ARPASTTLPA GADLADRFAE
    60 70 80 90 100
    LERRYDARLG VYVPATGTTA AIEYRADERF AFCSTFKAPL VAAVLHQNPL
    110 120 130 140 150
    THLDKLITYT SDDIRSISPV AQQHVQTGMT IGQLCDAAIR YSDGTAANLL
    160 170 180 190 200
    LADLGGPGGG TAAFTGYLRS LGDTVSRLDA EEPELNRDPP GDERDTTTPH
    210 220 230 240 250
    AIALVLQQLV LGNALPPDKR ALLTDWMARN TTGAKRIRAG FPADWKVIDK
    260 270 280 290 300
    TGTGDYGRAN DIAVVWSPTG VPYVVAVMSD RAGGGYDAEP REALLAEAAT

    CVAGVLA
    Length:307
    Mass (Da):32,568
    Last modified:July 10, 2007 - v1
    Checksum:i448CB2A0E05F4315
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U67924 Genomic DNA. Translation: AAB07556.1.
    CP000611 Genomic DNA. Translation: ABQ73843.1.
    RefSeqiWP_003410677.1. NG_047489.1.

    Genome annotation databases

    EnsemblBacteriaiABQ73843; ABQ73843; MRA_2082.
    KEGGimra:MRA_2082.
    PATRICi18144269. VBIMycTub106795_2337.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U67924 Genomic DNA. Translation: AAB07556.1.
    CP000611 Genomic DNA. Translation: ABQ73843.1.
    RefSeqiWP_003410677.1. NG_047489.1.

    3D structure databases

    ProteinModelPortaliA5U493.
    SMRiA5U493.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi419947.MtubH3_010100007057.

    Proteomic databases

    PRIDEiA5U493.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiABQ73843; ABQ73843; MRA_2082.
    KEGGimra:MRA_2082.
    PATRICi18144269. VBIMycTub106795_2337.

    Phylogenomic databases

    eggNOGiENOG4108J4B. Bacteria.
    COG2367. LUCA.
    HOGENOMiHOG000201073.
    KOiK17836.
    OMAiDEKEATY.

    Miscellaneous databases

    EvolutionaryTraceiA5U493.

    Family and domain databases

    Gene3Di3.40.710.10. 1 hit.
    InterProiIPR012338. Beta-lactam/transpept-like.
    IPR000871. Beta-lactam_class-A.
    IPR023650. Beta-lactam_class-A_AS.
    [Graphical view]
    PRINTSiPR00118. BLACTAMASEA.
    SUPFAMiSSF56601. SSF56601. 1 hit.
    PROSITEiPS00146. BETA_LACTAMASE_A. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiBLAC_MYCTA
    AccessioniPrimary (citable) accession number: A5U493
    Secondary accession number(s): P0A5I6, Q10670
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 11, 2007
    Last sequence update: July 10, 2007
    Last modified: November 2, 2016
    This is version 60 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.