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A5U493 (BLAC_MYCTA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Beta-lactamase
EC=3.5.2.6
Alternative name(s):
Penicillinase
Gene names
Name:blaA
Synonyms:blaC
Ordered Locus Names:MRA_2082
OrganismMycobacterium tuberculosis (strain ATCC 25177 / H37Ra) [Complete proteome] [HAMAP]
Taxonomic identifier419947 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex ›

Protein attributes

Sequence length307 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

A beta-lactam + H2O = a substituted beta-amino acid.

Subcellular location

Cell membrane; Lipid-anchor Potential.

Sequence similarities

Belongs to the class-A beta-lactamase family.

Ontologies

Keywords
   Biological processAntibiotic resistance
   Cellular componentCell membrane
Membrane
   DomainSignal
   Molecular functionHydrolase
   PTMLipoprotein
Palmitate
   Technical term3D-structure
Complete proteome
Gene Ontology (GO)
   Biological_processbeta-lactam antibiotic catabolic process

Inferred from electronic annotation. Source: InterPro

response to antibiotic

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentplasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionbeta-lactamase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 Potential
Chain24 – 307284Beta-lactamase
PRO_0000300061

Regions

Region250 – 2523Substrate binding By similarity

Sites

Active site841Acyl-ester intermediate By similarity

Amino acid modifications

Lipidation241N-palmitoyl cysteine Potential
Lipidation241S-diacylglycerol cysteine Potential

Secondary structure

1 .............................................. 307
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
A5U493 [UniParc].

Last modified July 10, 2007. Version 1.
Checksum: 448CB2A0E05F4315

FASTA30732,568
        10         20         30         40         50         60 
MRNRGFGRRE LLVAMAMLVS VTGCARHASG ARPASTTLPA GADLADRFAE LERRYDARLG 

        70         80         90        100        110        120 
VYVPATGTTA AIEYRADERF AFCSTFKAPL VAAVLHQNPL THLDKLITYT SDDIRSISPV 

       130        140        150        160        170        180 
AQQHVQTGMT IGQLCDAAIR YSDGTAANLL LADLGGPGGG TAAFTGYLRS LGDTVSRLDA 

       190        200        210        220        230        240 
EEPELNRDPP GDERDTTTPH AIALVLQQLV LGNALPPDKR ALLTDWMARN TTGAKRIRAG 

       250        260        270        280        290        300 
FPADWKVIDK TGTGDYGRAN DIAVVWSPTG VPYVVAVMSD RAGGGYDAEP REALLAEAAT 


CVAGVLA

« Hide

References

« Hide 'large scale' references
[1]"Cloning and sequence analysis of a class A beta-lactamase from Mycobacterium tuberculosis H37Ra."
Hackbarth C.J., Unsal I., Chambers H.F.
Antimicrob. Agents Chemother. 41:1182-1185(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Complete genomic sequence of Mycobacterium tuberculosis strain H37Ra, a non-pathogenic variant closely related to the well-characterized pathogenic strain H37Rv."
Wang S.Y., Zheng H.J., Lv L.D., Wang B.F., Zhang X.L., Pu S.Y., Zhu G.F., Wang H.H., Zhao G.P., Zhang Y.
Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 25177 / H37Ra.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U67924 Genomic DNA. Translation: AAB07556.1.
CP000611 Genomic DNA. Translation: ABQ73843.1.
RefSeqYP_001283405.1. NC_009525.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3DWZX-ray1.80A43-307[»]
ProteinModelPortalA5U493.
SMRA5U493. Positions 43-307.
ModBaseSearch...

Protein-protein interaction databases

STRING419947.MRA_2082.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABQ73843; ABQ73843; MRA_2082.
GeneID5212563.
KEGGmra:MRA_2082.
PATRIC18144269. VBIMycTub106795_2337.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2367.
HOGENOMHOG000201073.
KOK01467.
OMAEYGRAND.
ProtClustDBCLSK791603.

Enzyme and pathway databases

BioCycMTUB419947:GJ8N-2149-MONOMER.

Family and domain databases

Gene3D3.40.710.10. 1 hit.
InterProIPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A/D.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view]
PRINTSPR00118. BLACTAMASEA.
SUPFAMSSF56601. PBP_transp_fold. 1 hit.
PROSITEPS00146. BETA_LACTAMASE_A. 1 hit.
PS51257. PROKAR_LIPOPROTEIN. False negative.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceA5U493.

Entry information

Entry nameBLAC_MYCTA
AccessionPrimary (citable) accession number: A5U493
Secondary accession number(s): P0A5I6, Q10670
Entry history
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: July 10, 2007
Last modified: May 1, 2013
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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