A5U1Y3 (CYSD_MYCTA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sulfate adenylyltransferase subunit 2
EC=2.7.7.4

Alternative name(s):
ATP-sulfurylase small subunit
Sulfate adenylate transferase
Short name=SAT

Gene names

Name:	cysD
Ordered Locus Names:	MRA_1293

Organism

Mycobacterium tuberculosis (strain ATCC 25177 / H37Ra) [Complete proteome] [HAMAP]

Taxonomic identifier

419947 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	309 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + sulfate = diphosphate + adenylyl sulfate. HAMAP MF_00064
Pathway	Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 1/3. HAMAP MF_00064
Subunit structure	Heterodimer composed of CysD, the smaller subunit, and CysN By similarity.
Sequence similarities	Belongs to the PAPS reductase family. CysD subfamily.
Sequence caution	The sequence ABQ73033.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	sulfate reduction Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW sulfate adenylyltransferase (ATP) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 309	309	Sulfate adenylyltransferase subunit 2 HAMAP MF_00064		PRO_0000340207

Sequences

	Sequence		Length	Mass (Da)
	A5U1Y3 [UniParc]. Last modified June 10, 2008. Version 2. Checksum: 75D965D35F1EC284	FASTA	309	34,889
	10 20 30 40 50 60 MTSDVTVGPA PGQYQLSHLR LLEAEAIHVI REVAAEFERP VLLFSGGKDS IVMLHLALKA 70 80 90 100 110 120 FRPGRLPFPV MHVDTGHNFD EVIATRDELV AAAGVRLVVA SVQDDIDAGR VVETIPSRNP 130 140 150 160 170 180 IQTVTLLRAI RENQFDAAFG GARRDEEKAR AKERVFSFRD EFGQWDPKAQ RPELWNLYNG 190 200 210 220 230 240 RHHKGEHIRV FPLSNWTEFD IWSYIGAEQV RLPSIYFAHR RKVFQRDGML LAVHRHMQPR 250 260 270 280 290 300 ADEPVFEATV RFRTVGDVTC TGCVESSAST VAEVIAETAV ARLTERGATR ADDRISEAGM EDRKRQGYF

References

[1]

"Complete genomic sequence of Mycobacterium tuberculosis strain H37Ra, a non-pathogenic variant closely related to the well-characterized pathogenic strain H37Rv." Wang S.Y., Zheng H.J., Lv L.D., Wang B.F., Zhang X.L., Pu S.Y., Zhu G.F., Wang H.H., Zhao G.P., Zhang Y. Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25177 / H37Ra.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000611 Genomic DNA. Translation: ABQ73033.1. Different initiation.
RefSeq	YP_001282595.1. NC_009525.1.
3D structure databases
ProteinModelPortal	A5U1Y3.
SMR	A5U1Y3. Positions 14-218.
ModBase	Search...
Protein-protein interaction databases
STRING	A5U1Y3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000067160; EBMYCP00000065270; EBMYCG00000067155.
GeneID	5211857.
GenomeReviews	Gene locus MRA_1293 in contig CP000611_GR.
KEGG	mra:MRA_1293.
PATRIC	18142471. VBIMycTub106795_1442.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0175.
GeneTree	EBGT00050000016335.
HOGENOM	HBG758022.
ProtClustDB	PRK05253.
Enzyme and pathway databases
BioCyc	MTUB419947:MRA_1293-MONOMER.
Family and domain databases
HAMAP	MF_00064. Sulf_adenylyltr_sub2. [Tree]
InterPro	IPR002500. PAPS_reduct. IPR014729. Rossmann-like_a/b/a_fold. IPR011784. SO4_adenylTrfase_ssu. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KO	K00957.
Pfam	PF01507. PAPS_reduct. 1 hit. [Graphical view]
PIRSF	PIRSF002936. CysDAde_trans. 1 hit.
TIGRFAMs	TIGR02039. CysD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CYSD_MYCTA

Accession

Primary (citable) accession number: A5U1Y3

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 10, 2008
Last sequence update:	June 10, 2008
Last modified:	December 14, 2011
This is version 34 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program