A5U1I0 (METE_MYCTA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
EC=2.1.1.14

Alternative name(s):
Cobalamin-independent methionine synthase
Methionine synthase, vitamin-B12 independent isozyme

Gene names

Name:	metE
Ordered Locus Names:	MRA_1142

Organism

Mycobacterium tuberculosis (strain ATCC 25177 / H37Ra) [Complete proteome] [HAMAP]

Taxonomic identifier

419947 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	759 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity. HAMAP MF_00172
Catalytic activity	5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00172
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172
Sequence similarities	Belongs to the vitamin-B12 independent methionine synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Domain	Repeat
Ligand	Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 759	759	5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP MF_00172		PRO_1000017256
Sites
	Metal binding	647	1	Zinc By similarity
	Metal binding	649	1	Zinc By similarity
	Metal binding	732	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5U1I0 [UniParc]. Last modified July 10, 2007. Version 1. Checksum: 702F90BF79B25C8D	FASTA	759	81,582
	10 20 30 40 50 60 MTQPVRRQPF TATITGSPRI GPRRELKRAT EGYWAGRTSR SELEAVAATL RRDTWSALAA 70 80 90 100 110 120 AGLDSVPVNT FSYYDQMLDT AVLLGALPPR VSPVSDGLDR YFAAARGTDQ IAPLEMTKWF 130 140 150 160 170 180 DTNYHYLVPE IGPSTTFTLH PGKVLAELKE ALGQGIPARP VIIGPITFLL LSKAVDGAGA 190 200 210 220 230 240 PIERLEELVP VYSELLSLLA DGGAQWVQFD EPALVTDLSP DAPALAEAVY TALCSVSNRP 250 260 270 280 290 300 AIYVATYFGD PGAALPALAR TPVEAIGVDL VAGADTSVAG VPELAGKTLV AGVVDGRNVW 310 320 330 340 350 360 RTDLEAALGT LATLLGSAAT VAVSTSCSTL HVPYSLEPET DLDDALRSWL AFGAEKVREV 370 380 390 400 410 420 VVLARALRDG HDAVADEIAS SRAAIASRKR DPRLHNGQIR ARIEAIVASG AHRGNAAQRR 430 440 450 460 470 480 ASQDARLHLP PLPTTTIGSY PQTSAIRVAR AALRAGEIDE AEYVRRMRQE ITEVIALQER 490 500 510 520 530 540 LGLDVLVHGE PERNDMVQYF AEQLAGFFAT QNGWVQSYGS RCVRPPILYG DVSRPRAMTV 550 560 570 580 590 600 EWITYAQSLT DKPVKGMLTG PVTILAWSFV RDDQPLADTA NQVALAIRDE TVDLQSAGIA 610 620 630 640 650 660 VIQVDEPALR ELLPLRRADQ AEYLRWAVGA FRLATSGVSD ATQIHTHLCY SEFGEVIGAI 670 680 690 700 710 720 ADLDADVTSI EAARSHMEVL DDLNAIGFAN GVGPGVYDIH SPRVPSAEEM ADSLRAALRA 730 740 750 VPAERLWVNP DCGLKTRNVD EVTASLHNMV AAAREVRAG

References

[1]

"Complete genomic sequence of Mycobacterium tuberculosis strain H37Ra, a non-pathogenic variant closely related to the well-characterized pathogenic strain H37Rv." Wang S.Y., Zheng H.J., Lv L.D., Wang B.F., Zhang X.L., Pu S.Y., Zhu G.F., Wang H.H., Zhao G.P., Zhang Y. Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25177 / H37Ra.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000611 Genomic DNA. Translation: ABQ72880.1.
RefSeq	YP_001282442.1. NC_009525.1.
3D structure databases
ProteinModelPortal	A5U1I0.
ModBase	Search...
Protein-protein interaction databases
STRING	A5U1I0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000065124; EBMYCP00000063234; EBMYCG00000065119.
GeneID	5215355.
GenomeReviews	Gene locus MRA_1142 in contig CP000611_GR.
KEGG	mra:MRA_1142.
PATRIC	18142132. VBIMycTub106795_1273.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0620.
GeneTree	EBGT00050000017657.
HOGENOM	HBG287495.
OMA	RNIWRAN.
ProtClustDB	PRK05222.
Enzyme and pathway databases
BioCyc	MTUB419947:MRA_1142-MONOMER.
Family and domain databases
HAMAP	MF_00172. Meth_synth. [Tree]
InterPro	IPR013215. Cbl-indep_Met_Synth_N. IPR006276. Cobalamin-indep_Met_synthase. IPR002629. Methionine_synth. [Graphical view]
KO	K00549.
Pfam	PF08267. Meth_synt_1. 1 hit. PF01717. Meth_synt_2. 1 hit. [Graphical view]
PIRSF	PIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMs	TIGR01371. Met_syn_B12ind. 1 hit.
ProtoNet	Search...

Entry information

Entry name

METE_MYCTA

Accession

Primary (citable) accession number: A5U1I0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	July 10, 2007
Last modified:	December 14, 2011
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program