A5U123 (MPRA_MYCTA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Response regulator MprA

Alternative name(s):
Mycobacterial persistence regulator A

Gene names

Name:	mprA
Ordered Locus Names:	MRA_0988

Organism

Mycobacterium tuberculosis (strain ATCC 25177 / H37Ra) [Complete proteome] [HAMAP]

Taxonomic identifier

419947 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex › Mycobacterium tuberculosis

Protein attributes

Sequence length	230 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Member of the two-component regulatory system MprB/MprA which contributes to maintaining a balance among several systems involved in stress resistance and is required for establishment and maintenance of persistent infection in the host. Functions as a transcriptional regulator that recognizes a 19-bp nucleotide motif comprizing two loosely conserved 8-bp direct DNA-binding motif repeats separated by a 3-bp spacer region By similarity.
Subcellular location	Cytoplasm Potential.
Post-translational modification	Phosphorylated and dephosphorylated by MprB By similarity.
Sequence similarities	Contains 1 response regulatory domain.

Ontologies

Keywords
Biological process	Stress response Transcription Transcription regulation Two-component regulatory system Virulence
Cellular component	Cytoplasm
Ligand	DNA-binding
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	intracellular signal transduction Inferred from electronic annotation. Source: GOC pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW response to stress Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW phosphorelay response regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 230	230	Response regulator MprA		PRO_0000308428
Regions
	Domain	4 – 118	115	Response regulatory
Amino acid modifications
	Modified residue	48	1	4-aspartylphosphate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5U123 [UniParc]. Last modified July 10, 2007. Version 1. Checksum: 7A159FED949BE001	FASTA	230	25,894
	10 20 30 40 50 60 MSVRILVVDD DRAVRESLRR SLSFNGYSVE LAHDGVEALD MIASDRPDAL VLDVMMPRLD 70 80 90 100 110 120 GLEVCRQLRG TGDDLPILVL TARDSVSERV AGLDAGADDY LPKPFALEEL LARMRALLRR 130 140 150 160 170 180 TKPEDAAESM AMRFSDLTLD PVTREVNRGQ RRISLTRTEF ALLEMLIANP RRVLTRSRIL 190 200 210 220 230 EEVWGFDFPT SGNALEVYVG YLRRKTEADG EPRLIHTVRG VGYVLRETPP

References

[1]

"Complete genomic sequence of Mycobacterium tuberculosis strain H37Ra, a non-pathogenic variant closely related to the well-characterized pathogenic strain H37Rv." Wang S.Y., Zheng H.J., Lv L.D., Wang B.F., Zhang X.L., Pu S.Y., Zhu G.F., Wang H.H., Zhao G.P., Zhang Y. Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25177 / H37Ra.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000611 Genomic DNA. Translation: ABQ72723.1.
RefSeq	YP_001282285.1. NC_009525.1.
3D structure databases
ProteinModelPortal	A5U123.
SMR	A5U123. Positions 17-226.
ModBase	Search...
Protein-protein interaction databases
STRING	419947.MRA_0988.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABQ72723; ABQ72723; MRA_0988.
GeneID	5212587.
KEGG	mra:MRA_0988.
PATRIC	18141770. VBIMycTub106795_1095.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0745.
HOGENOM	HOG000034815.
KO	K07669.
OMA	MLMANPR.
ProtClustDB	CLSK790869.
Enzyme and pathway databases
BioCyc	MTUB419947:GJ8N-1019-MONOMER.
Family and domain databases
Gene3D	1.10.10.10. 1 hit.
InterPro	IPR011006. CheY-like_superfamily. IPR001867. Sig_transdc_resp-reg_C. IPR001789. Sig_transdc_resp-reg_receiver. IPR011991. WHTH_DNA-bd_dom. [Graphical view]
Pfam	PF00072. Response_reg. 1 hit. PF00486. Trans_reg_C. 1 hit. [Graphical view]
SMART	SM00448. REC. 1 hit. SM00862. Trans_reg_C. 1 hit. [Graphical view]
SUPFAM	SSF52172. CheY_like. 1 hit.
PROSITE	PS50110. RESPONSE_REGULATORY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MPRA_MYCTA

Accession

Primary (citable) accession number: A5U123

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 23, 2007
Last sequence update:	July 10, 2007
Last modified:	May 1, 2013
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program