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A5U029

- MMAA2_MYCTA

UniProt

A5U029 - MMAA2_MYCTA

Protein

Cyclopropane mycolic acid synthase MmaA2

Gene

mmaA2

Organism
Mycobacterium tuberculosis (strain ATCC 25177 / H37Ra)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 45 (01 Oct 2014)
      Sequence version 1 (10 Jul 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of a double bond to a cis cyclopropane ring at the distal position of an alpha mycolic acid via the transfer of a methylene group from S-adenosyl-L-methionine. MmaA2 also catalyzes the biosynthesis of the cis-cyclopropanated methoxymycolates. Cyclopropanated mycolic acids are key factors participating in cell envelope permeability, host immunomodulation and persistence.1 Publication

    Catalytic activityi

    S-adenosyl-L-methionine + phospholipid olefinic fatty acid = S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei136 – 1361S-adenosyl-L-methionine; via carbonyl oxygenBy similarity
    Active sitei269 – 2691By similarity

    GO - Molecular functioni

    1. cyclopropane-fatty-acyl-phospholipid synthase activity Source: UniProtKB
    2. methyltransferase activity Source: UniProtKB

    GO - Biological processi

    1. lipid biosynthetic process Source: UniProtKB

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Lipid biosynthesis, Lipid metabolism

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciMTUB419947:GJ8N-669-MONOMER.
    UniPathwayiUPA00915.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cyclopropane mycolic acid synthase MmaA2 (EC:2.1.1.79)
    Short name:
    CMAS
    Alternative name(s):
    Cyclopropane-fatty-acyl-phospholipid synthase
    Short name:
    CFA synthase
    Mycolic acid methyltransferase
    Short name:
    MA-MT
    S-adenosylmethionine-dependent methyltransferase
    Short name:
    AdoMet-MT
    Short name:
    SAM-MT
    Gene namesi
    Name:mmaA2
    Synonyms:mma2
    Ordered Locus Names:MRA_0655
    OrganismiMycobacterium tuberculosis (strain ATCC 25177 / H37Ra)
    Taxonomic identifieri419947 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
    ProteomesiUP000001988: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 287287Cyclopropane mycolic acid synthase MmaA2PRO_0000398361Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi419947.MRA_0655.

    Structurei

    3D structure databases

    ProteinModelPortaliA5U029.
    SMRiA5U029. Positions 3-287.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni33 – 342S-adenosyl-L-methionine bindingBy similarity
    Regioni72 – 743S-adenosyl-L-methionine bindingBy similarity
    Regioni94 – 996S-adenosyl-L-methionine bindingBy similarity
    Regioni123 – 1242S-adenosyl-L-methionine bindingBy similarity

    Sequence similaritiesi

    Belongs to the CFA/CMAS family.Curated

    Phylogenomic databases

    eggNOGiCOG2230.
    HOGENOMiHOG000245191.
    KOiK00574.
    OMAiKDRQYSC.
    OrthoDBiEOG6BGNZP.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    InterProiIPR003333. Mycolic_cyclopropane_synthase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PfamiPF02353. CMAS. 1 hit.
    [Graphical view]
    PIRSFiPIRSF003085. CMAS. 1 hit.
    SUPFAMiSSF53335. SSF53335. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    A5U029-1 [UniParc]FASTAAdd to Basket

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    MVNDLTPHFE DVQAHYDLSD DFFRLFLDPT QTYSCAHFER EDMTLEEAQI    50
    AKIDLALGKL GLQPGMTLLD IGCGWGATMR RAIAQYDVNV VGLTLSKNQA 100
    AHVQKSFDEM DTPRDRRVLL AGWEQFNEPV DRIVSIGAFE HFGHDRHADF 150
    FARAHKILPP DGVLLLHTIT GLTRQQMVDH GLPLTLWLAR FLKFIATEIF 200
    PGGQPPTIEM VEEQSAKTGF TLTRRQSLQP HYARTLDLWA EALQEHKSEA 250
    IAIQSEEVYE RYMKYLTGCA KLFRVGYIDV NQFTLAK 287
    Length:287
    Mass (Da):32,724
    Last modified:July 10, 2007 - v1
    Checksum:i669C3224C6B178C0
    GO

    Sequence cautioni

    The sequence AAC44617.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U66108 Genomic DNA. Translation: AAC44617.1. Different initiation.
    CP000611 Genomic DNA. Translation: ABQ72379.1.
    PIRiA70614.
    RefSeqiYP_001281941.1. NC_009525.1.

    Genome annotation databases

    EnsemblBacteriaiABQ72379; ABQ72379; MRA_0655.
    GeneIDi5211740.
    KEGGimra:MRA_0655.
    PATRICi18141002. VBIMycTub106795_0718.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U66108 Genomic DNA. Translation: AAC44617.1 . Different initiation.
    CP000611 Genomic DNA. Translation: ABQ72379.1 .
    PIRi A70614.
    RefSeqi YP_001281941.1. NC_009525.1.

    3D structure databases

    ProteinModelPortali A5U029.
    SMRi A5U029. Positions 3-287.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 419947.MRA_0655.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABQ72379 ; ABQ72379 ; MRA_0655 .
    GeneIDi 5211740.
    KEGGi mra:MRA_0655.
    PATRICi 18141002. VBIMycTub106795_0718.

    Phylogenomic databases

    eggNOGi COG2230.
    HOGENOMi HOG000245191.
    KOi K00574.
    OMAi KDRQYSC.
    OrthoDBi EOG6BGNZP.

    Enzyme and pathway databases

    UniPathwayi UPA00915 .
    BioCyci MTUB419947:GJ8N-669-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    InterProi IPR003333. Mycolic_cyclopropane_synthase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    Pfami PF02353. CMAS. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF003085. CMAS. 1 hit.
    SUPFAMi SSF53335. SSF53335. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "A common mechanism for the biosynthesis of methoxy and cyclopropyl mycolic acids in Mycobacterium tuberculosis."
      Yuan Y., Barry C.E. III
      Proc. Natl. Acad. Sci. U.S.A. 93:12828-12833(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION IN OXYGEN-CONTAINING MYCOLATES BIOSYNTHESIS.
    2. "Complete genomic sequence of Mycobacterium tuberculosis strain H37Ra, a non-pathogenic variant closely related to the well-characterized pathogenic strain H37Rv."
      Wang S.Y., Zheng H.J., Lv L.D., Wang B.F., Zhang X.L., Pu S.Y., Zhu G.F., Wang H.H., Zhao G.P., Zhang Y.
      Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 25177 / H37Ra.

    Entry informationi

    Entry nameiMMAA2_MYCTA
    AccessioniPrimary (citable) accession number: A5U029
    Secondary accession number(s): P72026
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 5, 2010
    Last sequence update: July 10, 2007
    Last modified: October 1, 2014
    This is version 45 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3