Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

A5U029

- MMAA2_MYCTA

UniProt

A5U029 - MMAA2_MYCTA

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Cyclopropane mycolic acid synthase MmaA2

Gene

mmaA2

Organism
Mycobacterium tuberculosis (strain ATCC 25177 / H37Ra)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of a double bond to a cis cyclopropane ring at the distal position of an alpha mycolic acid via the transfer of a methylene group from S-adenosyl-L-methionine. MmaA2 also catalyzes the biosynthesis of the cis-cyclopropanated methoxymycolates. Cyclopropanated mycolic acids are key factors participating in cell envelope permeability, host immunomodulation and persistence.1 Publication

Catalytic activityi

S-adenosyl-L-methionine + phospholipid olefinic fatty acid = S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei136 – 1361S-adenosyl-L-methionine; via carbonyl oxygenBy similarity
Active sitei269 – 2691By similarity

GO - Molecular functioni

  1. cyclopropane-fatty-acyl-phospholipid synthase activity Source: UniProtKB
  2. methyltransferase activity Source: UniProtKB

GO - Biological processi

  1. lipid biosynthetic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciMTUB419947:GJ8N-669-MONOMER.
UniPathwayiUPA00915.

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclopropane mycolic acid synthase MmaA2 (EC:2.1.1.79)
Short name:
CMAS
Alternative name(s):
Cyclopropane-fatty-acyl-phospholipid synthase
Short name:
CFA synthase
Mycolic acid methyltransferase
Short name:
MA-MT
S-adenosylmethionine-dependent methyltransferase
Short name:
AdoMet-MT
Short name:
SAM-MT
Gene namesi
Name:mmaA2
Synonyms:mma2
Ordered Locus Names:MRA_0655
OrganismiMycobacterium tuberculosis (strain ATCC 25177 / H37Ra)
Taxonomic identifieri419947 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
ProteomesiUP000001988: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 287287Cyclopropane mycolic acid synthase MmaA2PRO_0000398361Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi419947.MRA_0655.

Structurei

3D structure databases

ProteinModelPortaliA5U029.
SMRiA5U029. Positions 3-287.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni33 – 342S-adenosyl-L-methionine bindingBy similarity
Regioni72 – 743S-adenosyl-L-methionine bindingBy similarity
Regioni94 – 996S-adenosyl-L-methionine bindingBy similarity
Regioni123 – 1242S-adenosyl-L-methionine bindingBy similarity

Sequence similaritiesi

Belongs to the CFA/CMAS family.Curated

Phylogenomic databases

eggNOGiCOG2230.
HOGENOMiHOG000245191.
KOiK00574.
OMAiKDRQYSC.
OrthoDBiEOG6BGNZP.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR003333. Mycolic_cyclopropane_synthase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamiPF02353. CMAS. 1 hit.
[Graphical view]
PIRSFiPIRSF003085. CMAS. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.

Sequencei

Sequence statusi: Complete.

A5U029-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVNDLTPHFE DVQAHYDLSD DFFRLFLDPT QTYSCAHFER EDMTLEEAQI
60 70 80 90 100
AKIDLALGKL GLQPGMTLLD IGCGWGATMR RAIAQYDVNV VGLTLSKNQA
110 120 130 140 150
AHVQKSFDEM DTPRDRRVLL AGWEQFNEPV DRIVSIGAFE HFGHDRHADF
160 170 180 190 200
FARAHKILPP DGVLLLHTIT GLTRQQMVDH GLPLTLWLAR FLKFIATEIF
210 220 230 240 250
PGGQPPTIEM VEEQSAKTGF TLTRRQSLQP HYARTLDLWA EALQEHKSEA
260 270 280
IAIQSEEVYE RYMKYLTGCA KLFRVGYIDV NQFTLAK
Length:287
Mass (Da):32,724
Last modified:July 10, 2007 - v1
Checksum:i669C3224C6B178C0
GO

Sequence cautioni

The sequence AAC44617.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U66108 Genomic DNA. Translation: AAC44617.1. Different initiation.
CP000611 Genomic DNA. Translation: ABQ72379.1.
PIRiA70614.
RefSeqiYP_001281941.1. NC_009525.1.

Genome annotation databases

EnsemblBacteriaiABQ72379; ABQ72379; MRA_0655.
GeneIDi5211740.
KEGGimra:MRA_0655.
PATRICi18141002. VBIMycTub106795_0718.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U66108 Genomic DNA. Translation: AAC44617.1 . Different initiation.
CP000611 Genomic DNA. Translation: ABQ72379.1 .
PIRi A70614.
RefSeqi YP_001281941.1. NC_009525.1.

3D structure databases

ProteinModelPortali A5U029.
SMRi A5U029. Positions 3-287.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 419947.MRA_0655.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABQ72379 ; ABQ72379 ; MRA_0655 .
GeneIDi 5211740.
KEGGi mra:MRA_0655.
PATRICi 18141002. VBIMycTub106795_0718.

Phylogenomic databases

eggNOGi COG2230.
HOGENOMi HOG000245191.
KOi K00574.
OMAi KDRQYSC.
OrthoDBi EOG6BGNZP.

Enzyme and pathway databases

UniPathwayi UPA00915 .
BioCyci MTUB419947:GJ8N-669-MONOMER.

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
InterProi IPR003333. Mycolic_cyclopropane_synthase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
Pfami PF02353. CMAS. 1 hit.
[Graphical view ]
PIRSFi PIRSF003085. CMAS. 1 hit.
SUPFAMi SSF53335. SSF53335. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A common mechanism for the biosynthesis of methoxy and cyclopropyl mycolic acids in Mycobacterium tuberculosis."
    Yuan Y., Barry C.E. III
    Proc. Natl. Acad. Sci. U.S.A. 93:12828-12833(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION IN OXYGEN-CONTAINING MYCOLATES BIOSYNTHESIS.
  2. "Complete genomic sequence of Mycobacterium tuberculosis strain H37Ra, a non-pathogenic variant closely related to the well-characterized pathogenic strain H37Rv."
    Wang S.Y., Zheng H.J., Lv L.D., Wang B.F., Zhang X.L., Pu S.Y., Zhu G.F., Wang H.H., Zhao G.P., Zhang Y.
    Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 25177 / H37Ra.

Entry informationi

Entry nameiMMAA2_MYCTA
AccessioniPrimary (citable) accession number: A5U029
Secondary accession number(s): P72026
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: July 10, 2007
Last modified: October 1, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3