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A5U029 (MMAA2_MYCTA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 42. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cyclopropane mycolic acid synthase MmaA2

Short name=CMAS
EC=2.1.1.79
Alternative name(s):
Cyclopropane-fatty-acyl-phospholipid synthase
Short name=CFA synthase
Mycolic acid methyltransferase
Short name=MA-MT
S-adenosylmethionine-dependent methyltransferase
Short name=AdoMet-MT
Short name=SAM-MT
Gene names
Name:mmaA2
Synonyms:mma2
Ordered Locus Names:MRA_0655
OrganismMycobacterium tuberculosis (strain ATCC 25177 / H37Ra) [Complete proteome] [HAMAP]
Taxonomic identifier419947 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex

Protein attributes

Sequence length287 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the conversion of a double bond to a cis cyclopropane ring at the distal position of an alpha mycolic acid via the transfer of a methylene group from S-adenosyl-L-methionine. MmaA2 also catalyzes the biosynthesis of the cis-cyclopropanated methoxymycolates. Cyclopropanated mycolic acids are key factors participating in cell envelope permeability, host immunomodulation and persistence. Ref.1

Catalytic activity

S-adenosyl-L-methionine + phospholipid olefinic fatty acid = S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid.

Pathway

Lipid metabolism; mycolic acid biosynthesis.

Sequence similarities

Belongs to the CFA/CMAS family.

Sequence caution

The sequence AAC44617.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 287287Cyclopropane mycolic acid synthase MmaA2
PRO_0000398361

Regions

Region33 – 342S-adenosyl-L-methionine binding By similarity
Region72 – 743S-adenosyl-L-methionine binding By similarity
Region94 – 996S-adenosyl-L-methionine binding By similarity
Region123 – 1242S-adenosyl-L-methionine binding By similarity

Sites

Active site2691 By similarity
Binding site1361S-adenosyl-L-methionine; via carbonyl oxygen By similarity

Sequences

Sequence LengthMass (Da)Tools
A5U029 [UniParc].

Last modified July 10, 2007. Version 1.
Checksum: 669C3224C6B178C0

FASTA28732,724
        10         20         30         40         50         60 
MVNDLTPHFE DVQAHYDLSD DFFRLFLDPT QTYSCAHFER EDMTLEEAQI AKIDLALGKL 

        70         80         90        100        110        120 
GLQPGMTLLD IGCGWGATMR RAIAQYDVNV VGLTLSKNQA AHVQKSFDEM DTPRDRRVLL 

       130        140        150        160        170        180 
AGWEQFNEPV DRIVSIGAFE HFGHDRHADF FARAHKILPP DGVLLLHTIT GLTRQQMVDH 

       190        200        210        220        230        240 
GLPLTLWLAR FLKFIATEIF PGGQPPTIEM VEEQSAKTGF TLTRRQSLQP HYARTLDLWA 

       250        260        270        280 
EALQEHKSEA IAIQSEEVYE RYMKYLTGCA KLFRVGYIDV NQFTLAK 

« Hide

References

« Hide 'large scale' references
[1]"A common mechanism for the biosynthesis of methoxy and cyclopropyl mycolic acids in Mycobacterium tuberculosis."
Yuan Y., Barry C.E. III
Proc. Natl. Acad. Sci. U.S.A. 93:12828-12833(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION IN OXYGEN-CONTAINING MYCOLATES BIOSYNTHESIS.
[2]"Complete genomic sequence of Mycobacterium tuberculosis strain H37Ra, a non-pathogenic variant closely related to the well-characterized pathogenic strain H37Rv."
Wang S.Y., Zheng H.J., Lv L.D., Wang B.F., Zhang X.L., Pu S.Y., Zhu G.F., Wang H.H., Zhao G.P., Zhang Y.
Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 25177 / H37Ra.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U66108 Genomic DNA. Translation: AAC44617.1. Different initiation.
CP000611 Genomic DNA. Translation: ABQ72379.1.
PIRA70614.
RefSeqYP_001281941.1. NC_009525.1.

3D structure databases

ProteinModelPortalA5U029.
SMRA5U029. Positions 3-287.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING419947.MRA_0655.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABQ72379; ABQ72379; MRA_0655.
GeneID5211740.
KEGGmra:MRA_0655.
PATRIC18141002. VBIMycTub106795_0718.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2230.
HOGENOMHOG000245191.
KOK00574.
OMAWKQADTL.
OrthoDBEOG6BGNZP.
ProtClustDBCLSK790562.

Enzyme and pathway databases

BioCycMTUB419947:GJ8N-669-MONOMER.
UniPathwayUPA00915.

Family and domain databases

InterProIPR003333. Mycolic_cyclopropane_synthase.
[Graphical view]
PfamPF02353. CMAS. 1 hit.
[Graphical view]
PIRSFPIRSF003085. CMAS. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMMAA2_MYCTA
AccessionPrimary (citable) accession number: A5U029
Secondary accession number(s): P72026
Entry history
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: July 10, 2007
Last modified: April 16, 2014
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways