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A5TYT6

- PCKG_MYCTA

UniProt

A5TYT6 - PCKG_MYCTA

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Protein
Phosphoenolpyruvate carboxykinase [GTP]
Gene
pckG, MRA_0219
Organism
Mycobacterium tuberculosis (strain ATCC 25177 / H37Ra)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle By similarity.UniRule annotation

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Binds 1 manganese ion per subunit By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei81 – 811Substrate By similarity
Binding sitei222 – 2221Substrate; via amide nitrogen By similarity
Metal bindingi229 – 2291Manganese By similarity
Binding sitei229 – 2291Substrate By similarity
Metal bindingi249 – 2491Manganese; via tele nitrogen By similarity
Binding sitei271 – 2711Substrate By similarity
Active sitei273 – 2731 By similarity
Metal bindingi296 – 2961Manganese By similarity
Binding sitei389 – 3891GTP By similarity
Binding sitei420 – 4201GTP By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi272 – 2776GTP By similarity
Nucleotide bindingi515 – 5184GTP By similarity

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-HAMAP
  2. manganese ion binding Source: UniProtKB-HAMAP
  3. phosphoenolpyruvate carboxykinase (GTP) activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. gluconeogenesis Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

GTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMTUB419947:GJ8N-221-MONOMER.
UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP] (EC:4.1.1.32)
Short name:
PEP carboxykinase
Short name:
PEPCK
Gene namesi
Name:pckG
Ordered Locus Names:MRA_0219
OrganismiMycobacterium tuberculosis (strain ATCC 25177 / H37Ra)
Taxonomic identifieri419947 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
ProteomesiUP000001988: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 606606Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation
PRO_1000060296Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi419947.MRA_0219.

Structurei

3D structure databases

ProteinModelPortaliA5TYT6.
SMRiA5TYT6. Positions 12-605.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni387 – 3893Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1274.
HOGENOMiHOG000191700.
KOiK01596.
OMAiWDNPAGV.
OrthoDBiEOG6MPWQS.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A5TYT6-1 [UniParc]FASTAAdd to Basket

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MTSATIPGLD TAPTNHQGLL SWVEEVAELT QPDRVVFTDG SEEEFQRLCD    50
QLVEAGTFIR LNPEKHKNSY LALSDPSDVA RVESRTYICS AKEIDAGPTN 100
NWMDPGEMRS IMKDLYRGCM RGRTMYVVPF CMGPLGAEDP KLGVEITDSE 150
YVVVSMRTMT RMGKAALEKM GDDGFFVKAL HSVGAPLEPG QKDVAWPCSE 200
TKYITHFPET REIWSYGSGY GGNALLGKKC YSLRIASAMA HDEGWLAEHM 250
LILKLISPEN KAYYFAAAFP SACGKTNLAM LQPTIPGWRA ETLGDDIAWM 300
RFGKDGRLYA VNPEFGFFGV APGTNWKSNP NAMRTIAAGN TVFTNVALTD 350
DGDVWWEGLE GDPQHLIDWK GNDWYFRETE TNAAHPNSRY CTPMSQCPIL 400
APEWDDPQGV PISGILFGGR RKTTVPLVTE ARDWQHGVFI GATLGSEQTA 450
AAEGKVGNVR RDPMAMLPFL GYNVGDYFQH WINLGKHADE SKLPKVFFVN 500
WFRRGDDGRF LWPGFGENSR VLKWIVDRIE HKAGGATTPI GTVPAVEDLD 550
LDGLDVDAAD VAAALAVDAD EWRQELPLIE EWLQFVGEKL PTGVKDEFDA 600
LKERLG 606
Length:606
Mass (Da):67,253
Last modified:July 10, 2007 - v1
Checksum:iAEE29412E6BCCAE3
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000611 Genomic DNA. Translation: ABQ71936.1.
RefSeqiYP_001281498.1. NC_009525.1.

Genome annotation databases

EnsemblBacteriaiABQ71936; ABQ71936; MRA_0219.
GeneIDi5215145.
KEGGimra:MRA_0219.
PATRICi18140035. VBIMycTub106795_0239.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000611 Genomic DNA. Translation: ABQ71936.1 .
RefSeqi YP_001281498.1. NC_009525.1.

3D structure databases

ProteinModelPortali A5TYT6.
SMRi A5TYT6. Positions 12-605.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 419947.MRA_0219.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABQ71936 ; ABQ71936 ; MRA_0219 .
GeneIDi 5215145.
KEGGi mra:MRA_0219.
PATRICi 18140035. VBIMycTub106795_0239.

Phylogenomic databases

eggNOGi COG1274.
HOGENOMi HOG000191700.
KOi K01596.
OMAi WDNPAGV.
OrthoDBi EOG6MPWQS.

Enzyme and pathway databases

UniPathwayi UPA00138 .
BioCyci MTUB419947:GJ8N-221-MONOMER.

Family and domain databases

Gene3Di 3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPi MF_00452. PEPCK_GTP.
InterProi IPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view ]
PANTHERi PTHR11561. PTHR11561. 1 hit.
Pfami PF00821. PEPCK. 1 hit.
[Graphical view ]
PIRSFi PIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMi SSF68923. SSF68923. 1 hit.
PROSITEi PS00505. PEPCK_GTP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete genomic sequence of Mycobacterium tuberculosis strain H37Ra, a non-pathogenic variant closely related to the well-characterized pathogenic strain H37Rv."
    Wang S.Y., Zheng H.J., Lv L.D., Wang B.F., Zhang X.L., Pu S.Y., Zhu G.F., Wang H.H., Zhao G.P., Zhang Y.
    Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 25177 / H37Ra.

Entry informationi

Entry nameiPCKG_MYCTA
AccessioniPrimary (citable) accession number: A5TYT6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: July 10, 2007
Last modified: May 14, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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