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A5TYT3 (TRMB_MYCTA) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 50. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:MRA_0216
OrganismMycobacterium tuberculosis (strain ATCC 25177 / H37Ra) [Complete proteome] [HAMAP]
Taxonomic identifier419947 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex

Protein attributes

Sequence length263 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 263263tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_1000136357

Regions

Region232 – 2354Substrate binding By similarity

Sites

Active site1591 By similarity
Binding site821S-adenosyl-L-methionine By similarity
Binding site1071S-adenosyl-L-methionine By similarity
Binding site1361S-adenosyl-L-methionine By similarity
Binding site1591S-adenosyl-L-methionine By similarity
Binding site1631Substrate By similarity
Binding site1951Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
A5TYT3 [UniParc].

Last modified July 10, 2007. Version 1.
Checksum: 5CECF14C9650AF07

FASTA26328,547
        10         20         30         40         50         60 
MVHHGQMHAQ PGVGLRPDTP VASGQLPSTS IRSRRSGISK AQRETWERLW PELGLLALPQ 

        70         80         90        100        110        120 
SPRGTPVDTR AWFGRDAPVV LEIGSGSGTS TLAMAKAEPH VDVIAVDVYR RGLAQLLCAI 

       130        140        150        160        170        180 
DKVGSDGINI RLILGNAVDV LQHLIAPDSL CGVRVFFPDP WPKARHHKRR LLQPATMALI 

       190        200        210        220        230        240 
ADRLVPSGVL HAATDHPGYA EHIAAAGDAE PRLVRVDPDT ELLPISVVRP ATKYERKAQL 

       250        260 
GGGAVIELLW KKHGCSERDL KIR 

« Hide

References

[1]"Complete genomic sequence of Mycobacterium tuberculosis strain H37Ra, a non-pathogenic variant closely related to the well-characterized pathogenic strain H37Rv."
Wang S.Y., Zheng H.J., Lv L.D., Wang B.F., Zhang X.L., Pu S.Y., Zhu G.F., Wang H.H., Zhao G.P., Zhang Y.
Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 25177 / H37Ra.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000611 Genomic DNA. Translation: ABQ71933.1.
RefSeqYP_001281495.1. NC_009525.1.

3D structure databases

ProteinModelPortalA5TYT3.
SMRA5TYT3. Positions 47-235.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING419947.MRA_0216.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABQ71933; ABQ71933; MRA_0216.
GeneID5215142.
KEGGmra:MRA_0216.
PATRIC18140029. VBIMycTub106795_0236.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000073968.
KOK03439.
OMAFATDWEN.
OrthoDBEOG6K6VBC.

Enzyme and pathway databases

BioCycMTUB419947:GJ8N-218-MONOMER.
UniPathwayUPA00989.

Family and domain databases

Gene3D3.40.50.150. 1 hit.
HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMSSF53335. SSF53335. 1 hit.
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_MYCTA
AccessionPrimary (citable) accession number: A5TYT3
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: July 10, 2007
Last modified: June 11, 2014
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways