A5TYI7 (GMHA_MYCTA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoheptose isomerase
EC=5.3.1.28

Alternative name(s):
Sedoheptulose 7-phosphate isomerase

Gene names

Name:	gmhA
Ordered Locus Names:	MRA_0119

Organism

Mycobacterium tuberculosis (strain ATCC 25177 / H37Ra) [Complete proteome] [HAMAP]

Taxonomic identifier

419947 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	196 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the isomerization of sedoheptulose 7-phosphate in D-glycero-D-manno-heptose 7-phosphate By similarity. HAMAP MF_00067
Catalytic activity	D-sedoheptulose 7-phosphate = D-glycero-D-manno-heptose 7-phosphate. HAMAP MF_00067
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00067
Pathway	Carbohydrate biosynthesis; D-glycero-D-manno-heptose 7-phosphate biosynthesis; D-glycero-alpha-D-manno-heptose 7-phosphate and D-glycero-beta-D-manno-heptose 7-phosphate from sedoheptulose 7-phosphate: step 1/1. HAMAP MF_00067
Subcellular location	Cytoplasm By similarity HAMAP MF_00067.
Miscellaneous	The reaction produces a racemic mixture of D-glycero-alpha-D-manno-heptose 7-phosphate and D-glycero-beta-D-manno-heptose 7-phosphate By similarity. HAMAP MF_00067
Sequence similarities	Belongs to the SIS family. GmhA subfamily. Contains 1 SIS domain.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Cellular component	Cytoplasm
Ligand	Metal-binding Zinc
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	D-sedoheptulose 7-phosphate isomerase activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW sugar binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 196	196	Phosphoheptose isomerase HAMAP MF_00067		PRO_1000009081
Regions
	Domain	38 – 196	159	SIS
	Region	53 – 55	3	Substrate binding By similarity
	Region	95 – 96	2	Substrate binding By similarity
	Region	121 – 123	3	Substrate binding By similarity
Sites
	Metal binding	62	1	Zinc By similarity
	Metal binding	66	1	Zinc By similarity
	Metal binding	173	1	Zinc By similarity
	Metal binding	181	1	Zinc By similarity
	Binding site	66	1	Substrate By similarity
	Binding site	126	1	Substrate By similarity
	Binding site	173	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5TYI7 [UniParc]. Last modified July 10, 2007. Version 1. Checksum: 0F05EB968F1189CB	FASTA	196	20,923
	10 20 30 40 50 60 MCTARTAEEI FVETIAVKTR ILNDRVLLEA ARAIGDRLIA GYRAGARVFM CGNGGSAADA 70 80 90 100 110 120 QHFAAELTGH LIFDRPPLGA EALHANSSHL TAVANDYDYD TVFARALEGS ARPGDTLFAI 130 140 150 160 170 180 STSGNSMSVL RAAKTARELG VTVVAMTGES GGQLAEFADF LINVPSRDTG RIQESHIVFI 190 HAISEHVEHA LFAPRQ

References

[1]

"Complete genomic sequence of Mycobacterium tuberculosis strain H37Ra, a non-pathogenic variant closely related to the well-characterized pathogenic strain H37Rv." Wang S.Y., Zheng H.J., Lv L.D., Wang B.F., Zhang X.L., Pu S.Y., Zhu G.F., Wang H.H., Zhao G.P., Zhang Y. Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25177 / H37Ra.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000611 Genomic DNA. Translation: ABQ71837.1.
RefSeq	YP_001281399.1. NC_009525.1.
3D structure databases
ProteinModelPortal	A5TYI7.
ModBase	Search...
Protein-protein interaction databases
STRING	A5TYI7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000064852; EBMYCP00000062962; EBMYCG00000064847.
GeneID	5214713.
GenomeReviews	Gene locus MRA_0119 in contig CP000611_GR.
KEGG	mra:MRA_0119.
PATRIC	18139816. VBIMycTub106795_0129.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0279.
GeneTree	EBGT00050000016047.
HOGENOM	HBG671955.
OMA	HITIIHI.
ProtClustDB	PRK13938.
Enzyme and pathway databases
BioCyc	MTUB419947:MRA_0119-MONOMER.
Family and domain databases
HAMAP	MF_00067. GmhA. [Tree]
InterPro	IPR020620. Phosphoheptose_isomerase. IPR001347. SIS. [Graphical view]
KO	K03271.
Pfam	PF01380. SIS. 1 hit. [Graphical view]
PROSITE	PS51464. SIS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GMHA_MYCTA

Accession

Primary (citable) accession number: A5TYI7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	July 10, 2007
Last modified:	December 14, 2011
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program