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Protein

Ubiquitin carboxyl-terminal hydrolase 20

Gene

usp20

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Deubiquitinating enzyme involved in beta-2 adrenergic receptor (adrb2) recycling. Acts as a regulator of G-protein coupled receptor (GPCR) signaling by mediating the deubiquitination beta-2 adrenergic receptor (adrb2). Plays a central role in adrb2 recycling and resensitization after prolonged agonist stimulation by constitutively binding adrb2, mediating deubiquitination of adrb2 and inhibiting lysosomal trafficking of adrb2. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei157 – 1571NucleophilePROSITE-ProRule annotation
Active sitei643 – 6431Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri28 – 9265UBP-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. cysteine-type endopeptidase activity Source: UniProtKB
  2. ubiquitin-specific protease activity Source: InterPro
  3. zinc ion binding Source: InterPro

GO - Biological processi

  1. endocytosis Source: UniProtKB-KW
  2. protein deubiquitination Source: UniProtKB
  3. protein K48-linked deubiquitination Source: UniProtKB
  4. protein K63-linked deubiquitination Source: UniProtKB
  5. regulation of G-protein coupled receptor protein signaling pathway Source: UniProtKB
  6. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Endocytosis, Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiC19.025.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 20 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 20
Ubiquitin thioesterase 20
Ubiquitin-specific-processing protease 20
Gene namesi
Name:usp20
ORF Names:dkey-125i20.3
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
ProteomesiUP000000437 Componenti: Unplaced

Organism-specific databases

ZFINiZDB-GENE-040426-1219. usp20.

Subcellular locationi

GO - Cellular componenti

  1. centrosome Source: UniProtKB
  2. perinuclear region of cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 911911Ubiquitin carboxyl-terminal hydrolase 20PRO_0000390420Add
BLAST

Expressioni

Gene expression databases

BgeeiA5PN09.

Structurei

3D structure databases

ProteinModelPortaliA5PN09.
SMRiA5PN09. Positions 8-95.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini148 – 685538USPAdd
BLAST
Domaini687 – 78094DUSP 1PROSITE-ProRule annotationAdd
BLAST
Domaini789 – 891103DUSP 2PROSITE-ProRule annotationAdd
BLAST

Domaini

The UBP-type zinc finger binds 3 zinc ions. However, it does not bind ubiquitin, probably because the conserved Arg in position 55 is replaced by a Glu residue (By similarity).By similarity

Sequence similaritiesi

Contains 2 DUSP domains.PROSITE-ProRule annotation
Contains 1 UBP-type zinc finger.PROSITE-ProRule annotation
Contains 1 USP domain.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri28 – 9265UBP-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG5560.
HOGENOMiHOG000286031.
HOVERGENiHBG054196.
InParanoidiA5PN09.
KOiK11848.
PhylomeDBiA5PN09.

Family and domain databases

Gene3Di3.30.2230.10. 2 hits.
3.30.40.10. 1 hit.
InterProiIPR006615. Pept_C19_DUSP.
IPR001394. Peptidase_C19_UCH.
IPR018200. USP_CS.
IPR028889. USP_dom.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view]
PfamiPF06337. DUSP. 2 hits.
PF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view]
SMARTiSM00695. DUSP. 2 hits.
[Graphical view]
SUPFAMiSSF143791. SSF143791. 2 hits.
PROSITEiPS51283. DUSP. 2 hits.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A5PN09-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDSGDLCPH LDSIGEVTKE ELIQKSKGTC QSCGVGGPNL WACLQCDCPY
60 70 80 90 100
VGCGESYSDH STIHAQAKKH NLTVNLTTFR VWCYVCEREV FLEPKPVTPV
110 120 130 140 150
SSAHRCKPHD QDPVSQTTCY PLKAVPIAVA DEEGSESEED ELKPRGLTGM
160 170 180 190 200
KNIGNSCYMN AALQALSNCP PLTQFFQDCS GLVRTDKKPA LCKSYQKLIS
210 220 230 240 250
ELWHKKRPSY VVPTTLFHGI KLVNPMFRGY AQQDTQEFLR CLMDQLHEEL
260 270 280 290 300
KEPLFDCSGG ISEVEPDLSL DSCNLVDGDR SPSEDEFLSC DSGSGSERGD
310 320 330 340 350
GERAGGEAEL LIQDECVAVR GTGGISEKER LKERRGEERT REMDEDADVD
360 370 380 390 400
TAAQDGQAER ETETATPATA VPAPGNTEPD NEASMHCPSS RPCSPAHSVQ
410 420 430 440 450
ELHSRLSSNP PRSSPLRTGP TYTFKKAQML LSTKKKKQSR FRSVISDIFD
460 470 480 490 500
GSILSLVQCL TCDRVSTTVE TFQDLSLPIP GKEDLAKLHS SIHQSAPVKA
510 520 530 540 550
GVCTDGYAAQ GWISYIMDSI RRFVVSCIPS WFWGPMVTLE DCLAAFFAAD
560 570 580 590 600
ELKGDNMYSC ERCKKLRNGV KYCKVLRLPE ILCIHLKRFR HEVMYSFKIN
610 620 630 640 650
SHVSFPLEGL DLKPFLAKES PSQITTYDLL SVICHHGTAG SGHYIAYCQN
660 670 680 690 700
VINGQWYEFD DQYVTEVHET VVQNAEAYVL FYRKSSEESV RERQRVVALA
710 720 730 740 750
NLKEPSLLQF YISREWLNKF NTFTEPGPIT NHTFLCQHGG IPPTKYHYVD
760 770 780 790 800
DLVVILPQNV WEYLYNRFGG GPAVNHLYVC AICQVEIETL AKRRKLEIDT
810 820 830 840 850
FIKLNKEFQA EEAPTVILCI SMQWFREWEN FVKGKDNEPP GPIDNSKIAV
860 870 880 890 900
MKGGHIQLKQ GADYGQISEE TWQYLLSIYG GGPEIAVRQT ISPPDTDTHG
910
ERKIEAETRA L
Length:911
Mass (Da):101,984
Last modified:July 10, 2007 - v1
Checksum:iEB98B978FAE7FA4D
GO

Sequence cautioni

The sequence CAN88765.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti115 – 1151S → L in CAN88765 (PubMed:23594743).Curated
Sequence conflicti115 – 1151S → L in CAN88088 (PubMed:23594743).Curated
Sequence conflicti429 – 4291M → I in CAN88765 (PubMed:23594743).Curated
Sequence conflicti429 – 4291M → I in CAN88088 (PubMed:23594743).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX927184 Genomic DNA. Translation: CAN88489.1.
BX005029, CR382323 Genomic DNA. Translation: CAN88088.1.
CR382323, BX005029 Genomic DNA. Translation: CAN88765.1. Sequence problems.
RefSeqiNP_957281.2. NM_200987.2.
XP_009299733.1. XM_009301458.1.
UniGeneiDr.161818.

Genome annotation databases

GeneIDi393962.
KEGGidre:393962.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX927184 Genomic DNA. Translation: CAN88489.1.
BX005029, CR382323 Genomic DNA. Translation: CAN88088.1.
CR382323, BX005029 Genomic DNA. Translation: CAN88765.1. Sequence problems.
RefSeqiNP_957281.2. NM_200987.2.
XP_009299733.1. XM_009301458.1.
UniGeneiDr.161818.

3D structure databases

ProteinModelPortaliA5PN09.
SMRiA5PN09. Positions 8-95.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiC19.025.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi393962.
KEGGidre:393962.

Organism-specific databases

CTDi10868.
ZFINiZDB-GENE-040426-1219. usp20.

Phylogenomic databases

eggNOGiCOG5560.
HOGENOMiHOG000286031.
HOVERGENiHBG054196.
InParanoidiA5PN09.
KOiK11848.
PhylomeDBiA5PN09.

Miscellaneous databases

NextBioi20814933.
PROiA5PN09.

Gene expression databases

BgeeiA5PN09.

Family and domain databases

Gene3Di3.30.2230.10. 2 hits.
3.30.40.10. 1 hit.
InterProiIPR006615. Pept_C19_DUSP.
IPR001394. Peptidase_C19_UCH.
IPR018200. USP_CS.
IPR028889. USP_dom.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view]
PfamiPF06337. DUSP. 2 hits.
PF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view]
SMARTiSM00695. DUSP. 2 hits.
[Graphical view]
SUPFAMiSSF143791. SSF143791. 2 hits.
PROSITEiPS51283. DUSP. 2 hits.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The zebrafish reference genome sequence and its relationship to the human genome."
    Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M., Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I., Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.
    , White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y., Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B., Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S., Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M., Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J., Clee C., Oliver K., Clark R., Riddle C., Eliott D., Threadgold G., Harden G., Ware D., Mortimer B., Kerry G., Heath P., Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S., Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J., Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J., Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D., McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S., Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E., Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A., Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P., Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J., Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E., Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C., Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C., Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M., Oberlander M., Rudolph-Geiger S., Teucke M., Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M., Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M., de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C., Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.
    Nature 496:498-503(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Tuebingen.

Entry informationi

Entry nameiUBP20_DANRE
AccessioniPrimary (citable) accession number: A5PN09
Secondary accession number(s): A5PM59
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 2009
Last sequence update: July 10, 2007
Last modified: March 4, 2015
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.