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A5PN09

- UBP20_DANRE

UniProt

A5PN09 - UBP20_DANRE

Protein

Ubiquitin carboxyl-terminal hydrolase 20

Gene

usp20

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 53 (01 Oct 2014)
      Sequence version 1 (10 Jul 2007)
      Previous versions | rss
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    Functioni

    Deubiquitinating enzyme involved in beta-2 adrenergic receptor (adrb2) recycling. Acts as a regulator of G-protein coupled receptor (GPCR) signaling by mediating the deubiquitination beta-2 adrenergic receptor (adrb2). Plays a central role in adrb2 recycling and resensitization after prolonged agonist stimulation by constitutively binding adrb2, mediating deubiquitination of adrb2 and inhibiting lysosomal trafficking of adrb2. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains By similarity.By similarity

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei157 – 1571NucleophilePROSITE-ProRule annotation
    Active sitei643 – 6431Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri28 – 9265UBP-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. cysteine-type endopeptidase activity Source: UniProtKB
    2. ubiquitin-specific protease activity Source: InterPro
    3. ubiquitin thiolesterase activity Source: UniProtKB
    4. zinc ion binding Source: InterPro

    GO - Biological processi

    1. endocytosis Source: UniProtKB-KW
    2. protein deubiquitination Source: UniProtKB
    3. protein K48-linked deubiquitination Source: UniProtKB
    4. protein K63-linked deubiquitination Source: UniProtKB
    5. regulation of G-protein coupled receptor protein signaling pathway Source: UniProtKB
    6. ubiquitin-dependent protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Endocytosis, Ubl conjugation pathway

    Keywords - Ligandi

    Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin carboxyl-terminal hydrolase 20 (EC:3.4.19.12)
    Alternative name(s):
    Deubiquitinating enzyme 20
    Ubiquitin thioesterase 20
    Ubiquitin-specific-processing protease 20
    Gene namesi
    Name:usp20
    ORF Names:dkey-125i20.3
    OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
    Taxonomic identifieri7955 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
    ProteomesiUP000000437: Unplaced

    Organism-specific databases

    ZFINiZDB-GENE-040426-1219. usp20.

    Subcellular locationi

    GO - Cellular componenti

    1. centrosome Source: UniProtKB
    2. perinuclear region of cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 911911Ubiquitin carboxyl-terminal hydrolase 20PRO_0000390420Add
    BLAST

    Expressioni

    Gene expression databases

    BgeeiA5PN09.

    Structurei

    3D structure databases

    ProteinModelPortaliA5PN09.
    SMRiA5PN09. Positions 8-95.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini148 – 685538USPAdd
    BLAST
    Domaini687 – 78094DUSP 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini789 – 891103DUSP 2PROSITE-ProRule annotationAdd
    BLAST

    Domaini

    The UBP-type zinc finger binds 3 zinc ions. However, it does not bind ubiquitin, probably because the conserved Arg in position 55 is replaced by a Glu residue By similarity.By similarity

    Sequence similaritiesi

    Contains 2 DUSP domains.PROSITE-ProRule annotation
    Contains 1 UBP-type zinc finger.PROSITE-ProRule annotation
    Contains 1 USP domain.Curated

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri28 – 9265UBP-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5560.
    HOGENOMiHOG000286031.
    HOVERGENiHBG054196.
    KOiK11848.
    PhylomeDBiA5PN09.

    Family and domain databases

    Gene3Di3.30.2230.10. 2 hits.
    3.30.40.10. 1 hit.
    InterProiIPR006615. Pept_C19_DUSP.
    IPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    IPR013083. Znf_RING/FYVE/PHD.
    IPR001607. Znf_UBP.
    [Graphical view]
    PfamiPF06337. DUSP. 2 hits.
    PF00443. UCH. 1 hit.
    PF02148. zf-UBP. 1 hit.
    [Graphical view]
    SMARTiSM00695. DUSP. 2 hits.
    [Graphical view]
    SUPFAMiSSF143791. SSF143791. 2 hits.
    PROSITEiPS51283. DUSP. 2 hits.
    PS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    PS50271. ZF_UBP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A5PN09-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTDSGDLCPH LDSIGEVTKE ELIQKSKGTC QSCGVGGPNL WACLQCDCPY    50
    VGCGESYSDH STIHAQAKKH NLTVNLTTFR VWCYVCEREV FLEPKPVTPV 100
    SSAHRCKPHD QDPVSQTTCY PLKAVPIAVA DEEGSESEED ELKPRGLTGM 150
    KNIGNSCYMN AALQALSNCP PLTQFFQDCS GLVRTDKKPA LCKSYQKLIS 200
    ELWHKKRPSY VVPTTLFHGI KLVNPMFRGY AQQDTQEFLR CLMDQLHEEL 250
    KEPLFDCSGG ISEVEPDLSL DSCNLVDGDR SPSEDEFLSC DSGSGSERGD 300
    GERAGGEAEL LIQDECVAVR GTGGISEKER LKERRGEERT REMDEDADVD 350
    TAAQDGQAER ETETATPATA VPAPGNTEPD NEASMHCPSS RPCSPAHSVQ 400
    ELHSRLSSNP PRSSPLRTGP TYTFKKAQML LSTKKKKQSR FRSVISDIFD 450
    GSILSLVQCL TCDRVSTTVE TFQDLSLPIP GKEDLAKLHS SIHQSAPVKA 500
    GVCTDGYAAQ GWISYIMDSI RRFVVSCIPS WFWGPMVTLE DCLAAFFAAD 550
    ELKGDNMYSC ERCKKLRNGV KYCKVLRLPE ILCIHLKRFR HEVMYSFKIN 600
    SHVSFPLEGL DLKPFLAKES PSQITTYDLL SVICHHGTAG SGHYIAYCQN 650
    VINGQWYEFD DQYVTEVHET VVQNAEAYVL FYRKSSEESV RERQRVVALA 700
    NLKEPSLLQF YISREWLNKF NTFTEPGPIT NHTFLCQHGG IPPTKYHYVD 750
    DLVVILPQNV WEYLYNRFGG GPAVNHLYVC AICQVEIETL AKRRKLEIDT 800
    FIKLNKEFQA EEAPTVILCI SMQWFREWEN FVKGKDNEPP GPIDNSKIAV 850
    MKGGHIQLKQ GADYGQISEE TWQYLLSIYG GGPEIAVRQT ISPPDTDTHG 900
    ERKIEAETRA L 911
    Length:911
    Mass (Da):101,984
    Last modified:July 10, 2007 - v1
    Checksum:iEB98B978FAE7FA4D
    GO

    Sequence cautioni

    The sequence CAN88765.1 differs from that shown. Reason: Erroneous gene model prediction.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti115 – 1151S → L in CAN88765. (PubMed:23594743)Curated
    Sequence conflicti115 – 1151S → L in CAN88088. (PubMed:23594743)Curated
    Sequence conflicti429 – 4291M → I in CAN88765. (PubMed:23594743)Curated
    Sequence conflicti429 – 4291M → I in CAN88088. (PubMed:23594743)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX927184 Genomic DNA. Translation: CAN88489.1.
    BX005029, CR382323 Genomic DNA. Translation: CAN88088.1.
    CR382323, BX005029 Genomic DNA. Translation: CAN88765.1. Sequence problems.
    RefSeqiNP_957281.2. NM_200987.2.
    UniGeneiDr.161818.

    Genome annotation databases

    GeneIDi393962.
    KEGGidre:393962.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX927184 Genomic DNA. Translation: CAN88489.1 .
    BX005029 , CR382323 Genomic DNA. Translation: CAN88088.1 .
    CR382323 , BX005029 Genomic DNA. Translation: CAN88765.1 . Sequence problems.
    RefSeqi NP_957281.2. NM_200987.2.
    UniGenei Dr.161818.

    3D structure databases

    ProteinModelPortali A5PN09.
    SMRi A5PN09. Positions 8-95.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 393962.
    KEGGi dre:393962.

    Organism-specific databases

    CTDi 10868.
    ZFINi ZDB-GENE-040426-1219. usp20.

    Phylogenomic databases

    eggNOGi COG5560.
    HOGENOMi HOG000286031.
    HOVERGENi HBG054196.
    KOi K11848.
    PhylomeDBi A5PN09.

    Miscellaneous databases

    NextBioi 20814933.
    PROi A5PN09.

    Gene expression databases

    Bgeei A5PN09.

    Family and domain databases

    Gene3Di 3.30.2230.10. 2 hits.
    3.30.40.10. 1 hit.
    InterProi IPR006615. Pept_C19_DUSP.
    IPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    IPR013083. Znf_RING/FYVE/PHD.
    IPR001607. Znf_UBP.
    [Graphical view ]
    Pfami PF06337. DUSP. 2 hits.
    PF00443. UCH. 1 hit.
    PF02148. zf-UBP. 1 hit.
    [Graphical view ]
    SMARTi SM00695. DUSP. 2 hits.
    [Graphical view ]
    SUPFAMi SSF143791. SSF143791. 2 hits.
    PROSITEi PS51283. DUSP. 2 hits.
    PS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    PS50271. ZF_UBP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The zebrafish reference genome sequence and its relationship to the human genome."
      Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M., Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I., Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.
      , White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y., Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B., Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S., Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M., Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J., Clee C., Oliver K., Clark R., Riddle C., Eliott D., Threadgold G., Harden G., Ware D., Mortimer B., Kerry G., Heath P., Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S., Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J., Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J., Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D., McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S., Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E., Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A., Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P., Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J., Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E., Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C., Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C., Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M., Oberlander M., Rudolph-Geiger S., Teucke M., Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M., Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M., de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C., Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.
      Nature 496:498-503(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Tuebingen.

    Entry informationi

    Entry nameiUBP20_DANRE
    AccessioniPrimary (citable) accession number: A5PN09
    Secondary accession number(s): A5PM59
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 2009
    Last sequence update: July 10, 2007
    Last modified: October 1, 2014
    This is version 53 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3