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A5PMR2

- UBP33_DANRE

UniProt

A5PMR2 - UBP33_DANRE

Protein

Ubiquitin carboxyl-terminal hydrolase 33

Gene

usp33

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 52 (01 Oct 2014)
      Sequence version 1 (10 Jul 2007)
      Previous versions | rss
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    Functioni

    Deubiquitinating enzyme involved in various processes such as centrosome duplication, cellular migration and beta-2 adrenergic receptor/ADRB2 recycling. Involved in regulation of centrosome duplication by mediating deubiquitination of ccp110 in S and G2/M phase, leading to stabilize ccp110 during the period which centrioles duplicate and elongate. Involved in cell migration via its interaction with intracellular domain of robo1, leading to regulate the Slit signaling. Plays a role in commissural axon guidance cross the ventral midline of the neural tube in a Slit-dependent manner, possibly by mediating the deubiquitination of robo1. Acts as a regulator of G-protein coupled receptor (GPCR) signaling by mediating the deubiquitination of beta-arrestins (arrb1 and arrb2) and beta-2 adrenergic receptor (adrb2). Deubiquitinates dio2, thereby regulating thyroid hormone regulation. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains By similarity.By similarity

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei165 – 1651NucleophilePROSITE-ProRule annotation
    Active sitei628 – 6281Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri29 – 9365UBP-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. cysteine-type endopeptidase activity Source: UniProtKB
    2. ubiquitin-specific protease activity Source: UniProtKB
    3. ubiquitin thiolesterase activity Source: UniProtKB
    4. zinc ion binding Source: UniProtKB

    GO - Biological processi

    1. axon guidance Source: UniProtKB
    2. cell migration Source: UniProtKB
    3. centrosome duplication Source: UniProtKB
    4. endocytosis Source: UniProtKB-KW
    5. protein deubiquitination Source: UniProtKB
    6. protein K48-linked deubiquitination Source: UniProtKB
    7. protein K63-linked deubiquitination Source: UniProtKB
    8. regulation of G-protein coupled receptor protein signaling pathway Source: UniProtKB
    9. ubiquitin-dependent protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Endocytosis, Ubl conjugation pathway

    Keywords - Ligandi

    Metal-binding, Zinc

    Protein family/group databases

    MEROPSiC19.037.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin carboxyl-terminal hydrolase 33 (EC:3.4.19.12)
    Alternative name(s):
    Deubiquitinating enzyme 33
    Ubiquitin thioesterase 33
    Ubiquitin-specific-processing protease 33
    Gene namesi
    Name:usp33
    ORF Names:ch211-284g18.1
    OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
    Taxonomic identifieri7955 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
    ProteomesiUP000000437: Chromosome 2

    Organism-specific databases

    ZFINiZDB-GENE-040426-2323. usp33.

    Subcellular locationi

    Cytoplasmperinuclear region By similarity. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity
    Note: Associates with centrosomes predominantly in S and G2 phases but less in G1 phase.By similarity

    GO - Cellular componenti

    1. centrosome Source: UniProtKB
    2. perinuclear region of cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 897897Ubiquitin carboxyl-terminal hydrolase 33PRO_0000390426Add
    BLAST

    Expressioni

    Gene expression databases

    BgeeiA5PMR2.

    Structurei

    3D structure databases

    ProteinModelPortaliA5PMR2.
    SMRiA5PMR2. Positions 7-98.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini156 – 670515USPAdd
    BLAST
    Domaini672 – 76594DUSP 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini773 – 876104DUSP 2PROSITE-ProRule annotationAdd
    BLAST

    Domaini

    The UBP-type zinc finger binds 3 zinc ions. However, it does not bind ubiquitin, probably because the conserved Arg in position 55 is replaced by a Glu residue By similarity.By similarity

    Sequence similaritiesi

    Contains 2 DUSP domains.PROSITE-ProRule annotation
    Contains 1 UBP-type zinc finger.PROSITE-ProRule annotation
    Contains 1 USP domain.Curated

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri29 – 9365UBP-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5560.
    GeneTreeiENSGT00750000117701.
    HOGENOMiHOG000286031.
    HOVERGENiHBG054196.
    KOiK11848.
    OMAiLPSNEGV.
    OrthoDBiEOG7CRTP2.
    PhylomeDBiA5PMR2.
    TreeFamiTF352179.

    Family and domain databases

    Gene3Di3.30.2230.10. 2 hits.
    3.30.40.10. 1 hit.
    InterProiIPR006615. Pept_C19_DUSP.
    IPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    IPR013083. Znf_RING/FYVE/PHD.
    IPR001607. Znf_UBP.
    [Graphical view]
    PfamiPF06337. DUSP. 2 hits.
    PF00443. UCH. 1 hit.
    PF02148. zf-UBP. 1 hit.
    [Graphical view]
    SMARTiSM00695. DUSP. 2 hits.
    [Graphical view]
    SUPFAMiSSF143791. SSF143791. 2 hits.
    PROSITEiPS51283. DUSP. 2 hits.
    PS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    PS50271. ZF_UBP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A5PMR2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSGVSSNDCP HLECVGEITK EELIQKSHGQ CQDCKVGGPN LWACLENGCS    50
    YVGCGESHAD HSTVHSQETR HNLTVNLTTL RVWCYACTKE VFLERKLGPH 100
    KQLPNAAKAV SPVQTPCQDL NTPGSPTSLR VPSAGTCDDL DMETEEEDEL 150
    RTRGLTGLKN IGNTCYMNAA LQALSNCPPL TQFFLECGGL VKTDKKPALC 200
    KSYQKLITDL WHKNRNAYVV PTNLFQGIKA VNPMFRGYSQ QDSQEFLRCL 250
    MDQLHEELKE LIPEPEDPNQ AVAMDDSPDE DNHSQSDDFQ SCESCGSSDR 300
    ADNEGPRVPE DINEAEMLMP EQNQNNRDWQ KEKNLINNLY RAGSHGDLDK 350
    DVDTTSDSRP IISSQGAIKA QGRTSDSEIQ VSSTVRPQSP TGNEGITSRL 400
    SSSPPKSSAW PNLSSTHKKV PMFTPTKTKR QRKYHSIISE VFDGTIVSSV 450
    QCLTCDRVSV TLENFQDISL PIPGKEDLAK LHSSSHQTAL VKAGSCGEAY 500
    APQGWIAFVM EYIKSWFWGP VVTLQDCLAA FFARDELKGD NMYSCEKCKK 550
    LRNGVKFCKV QSLPEILCIH LKRFRHELMF STKIGTHVSF PLEGLEMQPF 600
    LAKDSSALTT TYDLLSVICH HGTASSGHYI AYCRNELNQL WYEFDDQSVT 650
    EVSESCVQNA EAYVLFYKKS NDETQKERRK VTSLFNMMEP SLLQFYVSRQ 700
    WLNKFKTFAE PGPISNHDFL CAHGGIPPNK AAYIDDLVLM IPQNVWDHLY 750
    SRYGGGPAVN HLYVCHTCQN EIEKLEKRRK NELDMFVRLN KAFQEEESPV 800
    VIYCISMQWF REWESFVKGK DIDPPGPIDN SKIAVNKNGH ITLKPGADSG 850
    QISEETWNFL HNIHGGGPVV TVRPSVSHQE SETSQSEEKI EVETRTV 897
    Length:897
    Mass (Da):100,655
    Last modified:July 10, 2007 - v1
    Checksum:i19103583F04B4315
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti207 – 2071I → V in AAH66557. 1 PublicationCurated
    Sequence conflicti217 – 2171A → S in AAH66557. 1 PublicationCurated
    Sequence conflicti732 – 7321A → T in AAH66557. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX649634 Genomic DNA. Translation: CAN88027.1.
    BC066557 mRNA. Translation: AAH66557.1.
    RefSeqiNP_998392.1. NM_213227.1.
    UniGeneiDr.32515.

    Genome annotation databases

    EnsembliENSDART00000018114; ENSDARP00000014516; ENSDARG00000016163.
    GeneIDi406508.
    KEGGidre:406508.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX649634 Genomic DNA. Translation: CAN88027.1 .
    BC066557 mRNA. Translation: AAH66557.1 .
    RefSeqi NP_998392.1. NM_213227.1.
    UniGenei Dr.32515.

    3D structure databases

    ProteinModelPortali A5PMR2.
    SMRi A5PMR2. Positions 7-98.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi C19.037.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSDART00000018114 ; ENSDARP00000014516 ; ENSDARG00000016163 .
    GeneIDi 406508.
    KEGGi dre:406508.

    Organism-specific databases

    CTDi 23032.
    ZFINi ZDB-GENE-040426-2323. usp33.

    Phylogenomic databases

    eggNOGi COG5560.
    GeneTreei ENSGT00750000117701.
    HOGENOMi HOG000286031.
    HOVERGENi HBG054196.
    KOi K11848.
    OMAi LPSNEGV.
    OrthoDBi EOG7CRTP2.
    PhylomeDBi A5PMR2.
    TreeFami TF352179.

    Miscellaneous databases

    NextBioi 20818084.
    PROi A5PMR2.

    Gene expression databases

    Bgeei A5PMR2.

    Family and domain databases

    Gene3Di 3.30.2230.10. 2 hits.
    3.30.40.10. 1 hit.
    InterProi IPR006615. Pept_C19_DUSP.
    IPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    IPR013083. Znf_RING/FYVE/PHD.
    IPR001607. Znf_UBP.
    [Graphical view ]
    Pfami PF06337. DUSP. 2 hits.
    PF00443. UCH. 1 hit.
    PF02148. zf-UBP. 1 hit.
    [Graphical view ]
    SMARTi SM00695. DUSP. 2 hits.
    [Graphical view ]
    SUPFAMi SSF143791. SSF143791. 2 hits.
    PROSITEi PS51283. DUSP. 2 hits.
    PS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    PS50271. ZF_UBP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The zebrafish reference genome sequence and its relationship to the human genome."
      Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M., Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I., Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.
      , White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y., Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B., Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S., Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M., Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J., Clee C., Oliver K., Clark R., Riddle C., Eliott D., Threadgold G., Harden G., Ware D., Mortimer B., Kerry G., Heath P., Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S., Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J., Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J., Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D., McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S., Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E., Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A., Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P., Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J., Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E., Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C., Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C., Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M., Oberlander M., Rudolph-Geiger S., Teucke M., Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M., Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M., de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C., Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.
      Nature 496:498-503(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Tuebingen.
    2. NIH - Zebrafish Gene Collection (ZGC) project
      Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Embryo.

    Entry informationi

    Entry nameiUBP33_DANRE
    AccessioniPrimary (citable) accession number: A5PMR2
    Secondary accession number(s): Q6NYK6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 2009
    Last sequence update: July 10, 2007
    Last modified: October 1, 2014
    This is version 52 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3