A5PMF6 (GT251_DANRE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Procollagen galactosyltransferase 1
EC=2.4.1.50

Alternative name(s):
Collagen beta(1-O)galactosyltransferase 1
Glycosyltransferase 25 family member 1
Hydroxylysine galactosyltransferase 1

Gene names

Name:	colgalt1
Synonyms:	glt25d1
ORF Names:	si:ch211-114l13.7, zgc:110667

Organism

Danio rerio (Zebrafish) (Brachydanio rerio) [Reference proteome]

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Actinopterygii › Actinopteri › Neopterygii › Teleostei › Elopocephala › Clupeocephala › Otocephala › Ostariophysi › Otophysi › Cypriniphysi › Cypriniformes › Cyprinoidea › Cyprinidae › Danio

Protein attributes

Sequence length	604 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Has a beta-galactosyltransferase activity; transfers beta-galactose to hydroxylysine residues of collagen By similarity.
Catalytic activity	UDP-alpha-D-galactose + 5-hydroxy-L-lysine-[procollagen] = UDP + 5-(D-galactosyloxy)-L-lysine-[procollagen].
Subcellular location	Endoplasmic reticulum lumen By similarity.
Sequence similarities	Belongs to the glycosyltransferase 25 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum
Domain	Signal
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	lipopolysaccharide biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular_component	endoplasmic reticulum lumen Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	procollagen galactosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Potential
	Chain	19 – 604	586	Procollagen galactosyltransferase 1		PRO_0000309538
Regions
	Motif	601 – 604	4	Prevents secretion from ER By similarity
Amino acid modifications
	Glycosylation	78	1	N-linked (GlcNAc...) Potential
	Glycosylation	166	1	N-linked (GlcNAc...) Potential
	Glycosylation	363	1	N-linked (GlcNAc...) Potential
	Glycosylation	561	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	A5PMF6 [UniParc]. Last modified July 10, 2007. Version 1. Checksum: 7D9556D629E4BF7D	FASTA	604	70,832
	10 20 30 40 50 60 MHLLCFFFLL LWTGPARSYF PEERWSPESA LLAPRVLVAL VCRNSAHSLP HVLGAIDRLN 70 80 90 100 110 120 YPKDRMAVWV ATDHNSDNTT EILREWLVNV QNFYHYVEWR PQDEPSVYEG ESGPKHWTNL 130 140 150 160 170 180 RYEHVMKLRQ AALETAREMW ADYFMLVDCD NLLTNRDVLW KLMRENKTIV APMLESRAAY 190 200 210 220 230 240 SNFWCGMTSQ GYYKRTPAYM PIRRQERKGC FAVPMVHSTL LLDLRKEASR QLAFFPPHPD 250 260 270 280 290 300 YTWAFDDIII FAFSARMAEV QMYICNRETY GYFPVPLRSQ NSLQDEAESF LHSQLEVMVR 310 320 330 340 350 360 NPPIEPSVYL SLMPKQTDKM GFDEVFMINL LRRSDRRERM LRTLYEQEIA CKIITAVDGK 370 380 390 400 410 420 ALNASQVEAL GIKMLPGYSD PYHGRPLTKG ELGCFLSHYN IWNEIVDRGL QSSLVIEDDL 430 440 450 460 470 480 RFEVFFKRRL QNLMQEVQSQ QLDWDLIYIG RKRMQVERPE KSVPRIHSLV EADYSYWTLG 490 500 510 520 530 540 YVISLRGAQK LLRAEPLKKM LPVDEFLPVM YNKHPIEEYM SHFPQRDLRA FSAEPLLIYP 550 560 570 580 590 600 THYTGDQGYI SDTETSSVWD NESVLTDWDR ARSRKSREQE ELSSEAQNTD VLQSPLDSTA RDEL

References

[1]	The Danio rerio sequencing project at the Sanger Institute Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX469930 Genomic DNA. Translation: CAN87888.1.
IPI	IPI00490992.
RefSeq	NP_001103992.1. NM_001110522.1.
UniGene	Dr.135672.
3D structure databases
ProteinModelPortal	A5PMF6.
ModBase	Search...
Protein-protein interaction databases
STRING	7955.ENSDARP00000075565.
Protein family/group databases
CAZy	GT25. Glycosyltransferase Family 25.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSDART00000081122; ENSDARP00000075565; ENSDARG00000058270.
GeneID	567859.
KEGG	dre:567859.
Phylogenomic databases
eggNOG	NOG293154.
GeneTree	ENSGT00550000074427.
HOGENOM	HOG000007198.
HOVERGEN	HBG058097.
OMA	QLEVMVK.
OrthoDB	EOG4B2SWX.
Gene expression databases
Bgee	A5PMF6.
Family and domain databases
InterPro	IPR002654. Glyco_trans_25. [Graphical view]
Pfam	PF01755. Glyco_transf_25. 1 hit. [Graphical view]
PROSITE	PS00014. ER_TARGET. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20888883.

Entry information

Entry name

GT251_DANRE

Accession

Primary (citable) accession number: A5PMF6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 13, 2007
Last sequence update:	July 10, 2007
Last modified:	May 1, 2013
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program