Skip Header

Contribute Send feedback
Read comments (?) or add your own

A5PKP9 (UB2D4_XENLA) Reviewed, UniProtKB/Swiss-Prot

Last modified March 6, 2013. Version 38. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin-conjugating enzyme E2 D4
EC=6.3.2.19
Alternative name(s):
Ubiquitin carrier protein D4
Ubiquitin-protein ligase D4
Gene names
Name:ube2d4
Synonyms:ube2d3.2
OrganismXenopus laevis (African clawed frog)
Taxonomic identifier8355 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length147 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the covalent attachment of ubiquitin to other proteins. Regulates pronephros development, possibly by promoting ubiquitination and thus inactivation or degradation of map3k10/mlk2. Ref.1

Catalytic activity

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine. UniProtKB Q9Y2X8

Pathway

Protein modification; protein ubiquitination. UniProtKB Q9Y2X8

Subunit structure

Interacts with map3k10/mlk2. Ref.1

Tissue specificity

At embryonic stages 28 to 35, expressed in the somites, eye primordia, otic vesicle and branchial arches. By stage 35, also weakly expressed in the pronephros. Ref.1

Developmental stage

Expressed maternally. Present at near constant levels throughout early development. Ref.1

Sequence similarities

Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
   Biological processUbl conjugation pathway
   LigandATP-binding
Nucleotide-binding
   Molecular functionDevelopmental protein
Ligase
Gene Ontology (GO)
   Biological_processpronephric nephron tubule development

Inferred from mutant phenotype Ref.1. Source: UniProtKB

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

ubiquitin binding

Inferred from direct assay Ref.1. Source: UniProtKB

ubiquitin-protein ligase activity

Inferred from direct assay Ref.1. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 147147Ubiquitin-conjugating enzyme E2 D4
PRO_0000397918

Sites

Active site851Glycyl thioester intermediate UniProtKB Q2T9X7

Experimental info

Mutagenesis851C → A: Catalytically inactive. No effect on pronephros development. Ref.1

Sequences

Sequence LengthMass (Da)Tools
A5PKP9 [UniParc].

Last modified July 10, 2007. Version 1.
Checksum: 3832EF718DF47EC2

FASTA14716,677
        10         20         30         40         50         60 
MALKRIQKEL MDLQRDPPAQ CSAGPVGEDL FHWQATIMGP NDSPFQGGVF FLTIHFPTDY 

        70         80         90        100        110        120 
PFKPPKVAFT TKIYHPNINS NGSICLDILR SQWSPALTVS KVLLSICSLL CDPNPDDPLV 

       130        140 
PEIAHTYKAD REKYNRLARE WTQKYAM

« Hide

References

« Hide 'large scale' references
[1]"A ubiquitin-conjugating enzyme, ube2d3.2, regulates xMLK2 and pronephros formation in Xenopus."
Jean S., Moss T.
Differentiation 76:431-441(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH MAP3K10, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, MUTAGENESIS OF CYS-85.
[2]NIH - Xenopus Gene Collection (XGC) project
Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Neurula.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		EU124673 mRNA. Translation: ABV25624.1.
BC142569 mRNA. Translation: AAI42570.1.
RefSeqNP_001093355.1. NM_001099885.1.
UniGeneXl.13069.

3D structure databases

ProteinModelPortalA5PKP9.
SMRA5PKP9. Positions 1-147.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID100101299.
KEGGxla:100101299.

Organism-specific databases

CTD51619.
XenbaseXB-GENE-962538. ube2d4.

Phylogenomic databases

HOVERGENHBG063308.
KOK06689.

Enzyme and pathway databases

UniPathwayUPA00143.

Family and domain databases

Gene3D3.10.110.10. 1 hit.
InterProIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMSSF54495. UBQ-conjugat/RWD-like. 1 hit.
PROSITEPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameUB2D4_XENLA
AccessionPrimary (citable) accession number: A5PKP9
Entry history
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: July 10, 2007
Last modified: March 6, 2013
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents