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A5PKJ4

- MK07_BOVIN

UniProt

A5PKJ4 - MK07_BOVIN

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Protein
Mitogen-activated protein kinase 7
Gene
MAPK7
Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Plays a role in various cellular processes such as proliferation, differentiation and cell survival. The upstream activator of MAPK7 is the MAPK kinase MAP2K5. Upon activation, it translocates to the nucleus and phosphorylates various downstream targets including MEF2C. EGF activates MAPK7 through a Ras-independent and MAP2K5-dependent pathway. May have a role in muscle cell differentiation. May be important for endothelial function and maintenance of blood vessel integrity. MAP2K5 and MAPK7 interact specifically with one another and not with MEK1/ERK1 or MEK2/ERK2 pathways. Phosphorylates SGK1 at Ser-78 and this is required for growth factor-induced cell cycle progression By similarity. Involved in the regulation of p53/TP53 by disrupting the PML-MDM2 interaction By similarity.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Magnesium By similarity.

Enzyme regulationi

Activated by tyrosine and threonine phosphorylation. Activated in response to hyperosmolarity, hydrogen peroxide, and epidermal growth factor (EGF) By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei84 – 841ATP By similarity
Active sitei182 – 1821Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi61 – 699ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. MAP kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. cell cycle Source: UniProtKB-KW
  2. cell differentiation Source: UniProtKB-KW
  3. cellular response to hydrogen peroxide Source: Ensembl
  4. cellular response to transforming growth factor beta stimulus Source: Ensembl
  5. negative regulation of NFAT protein import into nucleus Source: Ensembl
  6. negative regulation of endothelial cell apoptotic process Source: Ensembl
  7. negative regulation of extrinsic apoptotic signaling pathway in absence of ligand Source: Ensembl
  8. negative regulation of heterotypic cell-cell adhesion Source: Ensembl
  9. negative regulation of intrinsic apoptotic signaling pathway in response to oxidative stress Source: Ensembl
  10. negative regulation of response to cytokine stimulus Source: Ensembl
  11. peptidyl-serine phosphorylation Source: Ensembl
  12. positive regulation of protein metabolic process Source: Ensembl
  13. positive regulation of transcription from RNA polymerase II promoter in response to stress Source: Ensembl
  14. regulation of angiogenesis Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Cell cycle, Differentiation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_203215. Senescence-Associated Secretory Phenotype (SASP).
REACT_205036. Gastrin-CREB signalling pathway via PKC and MAPK.
REACT_220398. ERKs are inactivated.
REACT_226850. ERK/MAPK targets.
REACT_227858. Signalling to ERK5.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase 7 (EC:2.7.11.24)
Short name:
MAP kinase 7
Short name:
MAPK 7
Gene namesi
Name:MAPK7
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Chromosome 19

Subcellular locationi

Cytoplasm. Nucleus. NucleusPML body By similarity
Note: Translocates to the nucleus upon activation By similarity.

GO - Cellular componenti

  1. PML body Source: UniProtKB
  2. cytoplasm Source: UniProtKB
  3. cytosol Source: Ensembl
  4. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 781780Mitogen-activated protein kinase 7
PRO_0000349104Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine By similarity
Modified residuei685 – 6851Phosphoserine By similarity
Modified residuei698 – 6981Phosphothreonine By similarity

Post-translational modificationi

Dually phosphorylated on Thr-219 and Tyr-221, which activates the enzyme By similarity.

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PRIDEiA5PKJ4.

Interactioni

Subunit structurei

Interacts with MAP2K5. Forms oligomers By similarity. Interacts with MEF2A, MEF2C and MEF2D; the interaction phosphorylates the MEF2s and enhances transcriptional activity of MEF2A, MEF2C but not MEF2D By similarity. Interacts with SGK1 By similarity. Interacts with PML By similarity.

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000001347.

Structurei

3D structure databases

ProteinModelPortaliA5PKJ4.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini55 – 347293Protein kinase
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2 – 7776Required for cytoplasmic targeting By similarity
Add
BLAST
Regioni78 – 13962Required for binding to MAP2K5 By similarity
Add
BLAST
Regioni140 – 406267Necessary for oligomerization By similarity
Add
BLAST
Regioni407 – 781375May not be required for kinase activity; required to stimulate MEF2C activity By similarity
Add
BLAST
Regioni505 – 53935Nuclear localization signal By similarity
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi219 – 2213TXY By similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi403 – 46563Pro-rich
Add
BLAST
Compositional biasi513 – 54331Arg-rich
Add
BLAST
Compositional biasi578 – 66790Pro-rich
Add
BLAST

Domaini

The second proline-rich region may interact with actin targeting the kinase to a specific location in the cell.
The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00550000074298.
HOGENOMiHOG000113595.
HOVERGENiHBG108137.
InParanoidiA5PKJ4.
KOiK04464.
OMAiIIETIGT.
OrthoDBiEOG70PBX2.
TreeFamiTF105099.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A5PKJ4-1 [UniParc]FASTAAdd to Basket

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MAEPLKEDDG EDGSGEPPGP VKAEPAGTAA SVAAKNLALL KARSFDVTFD    50
VGDEYEIIET IGNGAYGVVS SARRRLTGQQ VAIKKIPNAF DVVTNAKRTL 100
RELKILKHFK HDNIIAIKDI LRPTVPYGEF KSVYVVLDLM ESDLHQIIHS 150
SQPLTLEHVR YFLYQLLRGL KYMHSAQVIH RDLKPSNLLV NENCELKIGD 200
FGMARGLCTS PAEHQYFMTE YVATRWYRAP ELMLSLHEYT QAIDLWSVGC 250
IFGEMLARRQ LFPGKNYVHQ LQLIMTVLGT PSPAVIQAVG AERVRAYIQS 300
LPPRQPVPWE TVYPGADRQA LSLLGRMLRF EPSARVSAAA ALRHPFLAKY 350
HDPDDEPDCA PPFDFAFDRE ALTRERIKEA IVAEIEDFHA RREGIRQQIR 400
FQPSLQPVAS EPGCPDVEMP SPWAPSGDCA MESPPPAPLP CPGPAPDTID 450
LTLQPPPPAS EPAPPKKEGA ISDNTKAALK AALLKSLRSR LRDGPSAPLE 500
APEPRKPVTA QERQREREEK RRRRQERAKE REKRRQERER KERGAGVSGG 550
PSADPLAGLV LSDNDRSLLE RWTRMAQPPA PAPATARPPS PPAGPATQPT 600
GPLPQPACPP PAPAAGPAAP QTTAASGLLA PQPLVPPPGL PGPSALSVLP 650
YFPSGPPPPD PGGAPQPSTS ESPDVTLVTQ QLSKSQVEDP LPPVFSGTPK 700
GSGAGYGVGF DLEEFLNQSF DMGVADGPQD GQADSASLSA SLLADWLEGH 750
GMNPADIESL QREIQMDSPM LLADLPDLQE P 781
Length:781
Mass (Da):84,832
Last modified:July 10, 2007 - v1
Checksum:i1BDFC98A7EA3751C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC142510 mRNA. Translation: AAI42511.1.
RefSeqiNP_001092550.1. NM_001099080.2.
XP_005220440.1. XM_005220383.1.
UniGeneiBt.103114.

Genome annotation databases

EnsembliENSBTAT00000001347; ENSBTAP00000001347; ENSBTAG00000001014.
GeneIDi537703.
KEGGibta:537703.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC142510 mRNA. Translation: AAI42511.1 .
RefSeqi NP_001092550.1. NM_001099080.2.
XP_005220440.1. XM_005220383.1.
UniGenei Bt.103114.

3D structure databases

ProteinModelPortali A5PKJ4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9913.ENSBTAP00000001347.

Proteomic databases

PRIDEi A5PKJ4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSBTAT00000001347 ; ENSBTAP00000001347 ; ENSBTAG00000001014 .
GeneIDi 537703.
KEGGi bta:537703.

Organism-specific databases

CTDi 5598.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00550000074298.
HOGENOMi HOG000113595.
HOVERGENi HBG108137.
InParanoidi A5PKJ4.
KOi K04464.
OMAi IIETIGT.
OrthoDBi EOG70PBX2.
TreeFami TF105099.

Enzyme and pathway databases

Reactomei REACT_203215. Senescence-Associated Secretory Phenotype (SASP).
REACT_205036. Gastrin-CREB signalling pathway via PKC and MAPK.
REACT_220398. ERKs are inactivated.
REACT_226850. ERK/MAPK targets.
REACT_227858. Signalling to ERK5.

Miscellaneous databases

NextBioi 20877197.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Fetal liver.

Entry informationi

Entry nameiMK07_BOVIN
AccessioniPrimary (citable) accession number: A5PKJ4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: July 10, 2007
Last modified: September 3, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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