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A5PKJ4 (MK07_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mitogen-activated protein kinase 7

Short name=MAP kinase 7
Short name=MAPK 7
EC=2.7.11.24
Gene names
Name:MAPK7
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length781 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Plays a role in various cellular processes such as proliferation, differentiation and cell survival. The upstream activator of MAPK7 is the MAPK kinase MAP2K5. Upon activation, it translocates to the nucleus and phosphorylates various downstream targets including MEF2C. EGF activates MAPK7 through a Ras-independent and MAP2K5-dependent pathway. May have a role in muscle cell differentiation. May be important for endothelial function and maintenance of blood vessel integrity. MAP2K5 and MAPK7 interact specifically with one another and not with MEK1/ERK1 or MEK2/ERK2 pathways. Phosphorylates SGK1 at Ser-78 and this is required for growth factor-induced cell cycle progression By similarity. Involved in the regulation of p53/TP53 by disrupting the PML-MDM2 interaction By similarity.

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Cofactor

Magnesium By similarity.

Enzyme regulation

Activated by tyrosine and threonine phosphorylation. Activated in response to hyperosmolarity, hydrogen peroxide, and epidermal growth factor (EGF) By similarity.

Subunit structure

Interacts with MAP2K5. Forms oligomers By similarity. Interacts with MEF2A, MEF2C and MEF2D; the interaction phosphorylates the MEF2s and enhances transcriptional activity of MEF2A, MEF2C but not MEF2D By similarity. Interacts with SGK1 By similarity. Interacts with PML By similarity.

Subcellular location

Cytoplasm. Nucleus. NucleusPML body By similarity. Note: Translocates to the nucleus upon activation By similarity.

Domain

The second proline-rich region may interact with actin targeting the kinase to a specific location in the cell.

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Post-translational modification

Dually phosphorylated on Thr-219 and Tyr-221, which activates the enzyme By similarity.

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.

Contains 1 protein kinase domain.

Ontologies

Keywords
   Biological processCell cycle
Differentiation
   Cellular componentCytoplasm
Nucleus
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Serine/threonine-protein kinase
Transferase
   PTMAcetylation
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcell cycle

Inferred from electronic annotation. Source: UniProtKB-KW

cell differentiation

Inferred from electronic annotation. Source: UniProtKB-KW

cellular response to hydrogen peroxide

Inferred from electronic annotation. Source: Ensembl

cellular response to transforming growth factor beta stimulus

Inferred from electronic annotation. Source: Ensembl

negative regulation of NFAT protein import into nucleus

Inferred from electronic annotation. Source: Ensembl

negative regulation of endothelial cell apoptotic process

Inferred from electronic annotation. Source: Ensembl

negative regulation of extrinsic apoptotic signaling pathway in absence of ligand

Inferred from electronic annotation. Source: Ensembl

negative regulation of heterotypic cell-cell adhesion

Inferred from electronic annotation. Source: Ensembl

negative regulation of intrinsic apoptotic signaling pathway in response to oxidative stress

Inferred from electronic annotation. Source: Ensembl

negative regulation of response to cytokine stimulus

Inferred from electronic annotation. Source: Ensembl

peptidyl-serine phosphorylation

Inferred from electronic annotation. Source: Ensembl

positive regulation of protein metabolic process

Inferred from electronic annotation. Source: Ensembl

positive regulation of transcription from RNA polymerase II promoter in response to stress

Inferred from electronic annotation. Source: Ensembl

regulation of angiogenesis

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentPML body

Inferred from sequence or structural similarity. Source: UniProtKB

cytoplasm

Inferred from sequence or structural similarity. Source: UniProtKB

cytosol

Inferred from electronic annotation. Source: Ensembl

nucleus

Inferred from sequence or structural similarity. Source: UniProtKB

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

MAP kinase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 781780Mitogen-activated protein kinase 7
PRO_0000349104

Regions

Domain55 – 347293Protein kinase
Nucleotide binding61 – 699ATP By similarity
Region2 – 7776Required for cytoplasmic targeting By similarity
Region78 – 13962Required for binding to MAP2K5 By similarity
Region140 – 406267Necessary for oligomerization By similarity
Region407 – 781375May not be required for kinase activity; required to stimulate MEF2C activity By similarity
Region505 – 53935Nuclear localization signal By similarity
Motif219 – 2213TXY By similarity
Compositional bias403 – 46563Pro-rich
Compositional bias513 – 54331Arg-rich
Compositional bias578 – 66790Pro-rich

Sites

Active site1821Proton acceptor By similarity
Binding site841ATP By similarity

Amino acid modifications

Modified residue21N-acetylalanine By similarity
Modified residue6851Phosphoserine By similarity
Modified residue6981Phosphothreonine By similarity

Sequences

Sequence LengthMass (Da)Tools
A5PKJ4 [UniParc].

Last modified July 10, 2007. Version 1.
Checksum: 1BDFC98A7EA3751C

FASTA78184,832
        10         20         30         40         50         60 
MAEPLKEDDG EDGSGEPPGP VKAEPAGTAA SVAAKNLALL KARSFDVTFD VGDEYEIIET 

        70         80         90        100        110        120 
IGNGAYGVVS SARRRLTGQQ VAIKKIPNAF DVVTNAKRTL RELKILKHFK HDNIIAIKDI 

       130        140        150        160        170        180 
LRPTVPYGEF KSVYVVLDLM ESDLHQIIHS SQPLTLEHVR YFLYQLLRGL KYMHSAQVIH 

       190        200        210        220        230        240 
RDLKPSNLLV NENCELKIGD FGMARGLCTS PAEHQYFMTE YVATRWYRAP ELMLSLHEYT 

       250        260        270        280        290        300 
QAIDLWSVGC IFGEMLARRQ LFPGKNYVHQ LQLIMTVLGT PSPAVIQAVG AERVRAYIQS 

       310        320        330        340        350        360 
LPPRQPVPWE TVYPGADRQA LSLLGRMLRF EPSARVSAAA ALRHPFLAKY HDPDDEPDCA 

       370        380        390        400        410        420 
PPFDFAFDRE ALTRERIKEA IVAEIEDFHA RREGIRQQIR FQPSLQPVAS EPGCPDVEMP 

       430        440        450        460        470        480 
SPWAPSGDCA MESPPPAPLP CPGPAPDTID LTLQPPPPAS EPAPPKKEGA ISDNTKAALK 

       490        500        510        520        530        540 
AALLKSLRSR LRDGPSAPLE APEPRKPVTA QERQREREEK RRRRQERAKE REKRRQERER 

       550        560        570        580        590        600 
KERGAGVSGG PSADPLAGLV LSDNDRSLLE RWTRMAQPPA PAPATARPPS PPAGPATQPT 

       610        620        630        640        650        660 
GPLPQPACPP PAPAAGPAAP QTTAASGLLA PQPLVPPPGL PGPSALSVLP YFPSGPPPPD 

       670        680        690        700        710        720 
PGGAPQPSTS ESPDVTLVTQ QLSKSQVEDP LPPVFSGTPK GSGAGYGVGF DLEEFLNQSF 

       730        740        750        760        770        780 
DMGVADGPQD GQADSASLSA SLLADWLEGH GMNPADIESL QREIQMDSPM LLADLPDLQE 


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References

[1]NIH - Mammalian Gene Collection (MGC) project
Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Hereford.
Tissue: Fetal liver.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC142510 mRNA. Translation: AAI42511.1.
RefSeqNP_001092550.1. NM_001099080.2.
XP_005220440.1. XM_005220383.1.
UniGeneBt.103114.

3D structure databases

ProteinModelPortalA5PKJ4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9913.ENSBTAP00000001347.

Proteomic databases

PRIDEA5PKJ4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSBTAT00000001347; ENSBTAP00000001347; ENSBTAG00000001014.
GeneID537703.
KEGGbta:537703.

Organism-specific databases

CTD5598.

Phylogenomic databases

eggNOGCOG0515.
GeneTreeENSGT00550000074298.
HOGENOMHOG000113595.
HOVERGENHBG108137.
InParanoidA5PKJ4.
KOK04464.
OMAIIETIGT.
OrthoDBEOG70PBX2.
TreeFamTF105099.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20877197.

Entry information

Entry nameMK07_BOVIN
AccessionPrimary (citable) accession number: A5PKJ4
Entry history
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: July 10, 2007
Last modified: April 16, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families