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A5PKJ4

- MK07_BOVIN

UniProt

A5PKJ4 - MK07_BOVIN

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Protein

Mitogen-activated protein kinase 7

Gene

MAPK7

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Plays a role in various cellular processes such as proliferation, differentiation and cell survival. The upstream activator of MAPK7 is the MAPK kinase MAP2K5. Upon activation, it translocates to the nucleus and phosphorylates various downstream targets including MEF2C. EGF activates MAPK7 through a Ras-independent and MAP2K5-dependent pathway. May have a role in muscle cell differentiation. May be important for endothelial function and maintenance of blood vessel integrity. MAP2K5 and MAPK7 interact specifically with one another and not with MEK1/ERK1 or MEK2/ERK2 pathways. Phosphorylates SGK1 at Ser-78 and this is required for growth factor-induced cell cycle progression (By similarity). Involved in the regulation of p53/TP53 by disrupting the PML-MDM2 interaction (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Mg2+By similarity

Enzyme regulationi

Activated by tyrosine and threonine phosphorylation. Activated in response to hyperosmolarity, hydrogen peroxide, and epidermal growth factor (EGF) (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei84 – 841ATPPROSITE-ProRule annotation
Active sitei182 – 1821Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi61 – 699ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. MAP kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. cell cycle Source: UniProtKB-KW
  2. cell differentiation Source: UniProtKB-KW
  3. cellular response to hydrogen peroxide Source: Ensembl
  4. cellular response to transforming growth factor beta stimulus Source: Ensembl
  5. negative regulation of endothelial cell apoptotic process Source: Ensembl
  6. negative regulation of extrinsic apoptotic signaling pathway in absence of ligand Source: Ensembl
  7. negative regulation of heterotypic cell-cell adhesion Source: Ensembl
  8. negative regulation of NFAT protein import into nucleus Source: Ensembl
  9. negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway Source: Ensembl
  10. negative regulation of response to cytokine stimulus Source: Ensembl
  11. peptidyl-serine phosphorylation Source: Ensembl
  12. positive regulation of protein metabolic process Source: Ensembl
  13. positive regulation of transcription from RNA polymerase II promoter in response to stress Source: Ensembl
  14. regulation of angiogenesis Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Cell cycle, Differentiation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_203215. Senescence-Associated Secretory Phenotype (SASP).
REACT_205036. Gastrin-CREB signalling pathway via PKC and MAPK.
REACT_220398. ERKs are inactivated.
REACT_226850. ERK/MAPK targets.
REACT_227858. Signalling to ERK5.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase 7 (EC:2.7.11.24)
Short name:
MAP kinase 7
Short name:
MAPK 7
Gene namesi
Name:MAPK7
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Chromosome 19

Subcellular locationi

Cytoplasm. Nucleus. NucleusPML body By similarity
Note: Translocates to the nucleus upon activation.By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. cytosol Source: Ensembl
  3. nucleus Source: UniProtKB
  4. PML body Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 781780Mitogen-activated protein kinase 7PRO_0000349104Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei685 – 6851PhosphoserineBy similarity
Modified residuei698 – 6981PhosphothreonineBy similarity

Post-translational modificationi

Dually phosphorylated on Thr-219 and Tyr-221, which activates the enzyme.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PRIDEiA5PKJ4.

Interactioni

Subunit structurei

Interacts with MAP2K5. Forms oligomers (By similarity). Interacts with MEF2A, MEF2C and MEF2D; the interaction phosphorylates the MEF2s and enhances transcriptional activity of MEF2A, MEF2C but not MEF2D (By similarity). Interacts with SGK1 (By similarity). Interacts with PML (By similarity).By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000001347.

Structurei

3D structure databases

ProteinModelPortaliA5PKJ4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini55 – 347293Protein kinasePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2 – 7776Required for cytoplasmic targetingBy similarityAdd
BLAST
Regioni78 – 13962Required for binding to MAP2K5By similarityAdd
BLAST
Regioni140 – 406267Necessary for oligomerizationBy similarityAdd
BLAST
Regioni407 – 781375May not be required for kinase activity; required to stimulate MEF2C activityBy similarityAdd
BLAST
Regioni505 – 53935Nuclear localization signalBy similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi219 – 2213TXYBy similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi403 – 46563Pro-richAdd
BLAST
Compositional biasi513 – 54331Arg-richAdd
BLAST
Compositional biasi578 – 66790Pro-richAdd
BLAST

Domaini

The second proline-rich region may interact with actin targeting the kinase to a specific location in the cell.
The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00550000074298.
HOGENOMiHOG000113595.
HOVERGENiHBG108137.
InParanoidiA5PKJ4.
KOiK04464.
OMAiIIETIGT.
OrthoDBiEOG70PBX2.
TreeFamiTF105099.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A5PKJ4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAEPLKEDDG EDGSGEPPGP VKAEPAGTAA SVAAKNLALL KARSFDVTFD
60 70 80 90 100
VGDEYEIIET IGNGAYGVVS SARRRLTGQQ VAIKKIPNAF DVVTNAKRTL
110 120 130 140 150
RELKILKHFK HDNIIAIKDI LRPTVPYGEF KSVYVVLDLM ESDLHQIIHS
160 170 180 190 200
SQPLTLEHVR YFLYQLLRGL KYMHSAQVIH RDLKPSNLLV NENCELKIGD
210 220 230 240 250
FGMARGLCTS PAEHQYFMTE YVATRWYRAP ELMLSLHEYT QAIDLWSVGC
260 270 280 290 300
IFGEMLARRQ LFPGKNYVHQ LQLIMTVLGT PSPAVIQAVG AERVRAYIQS
310 320 330 340 350
LPPRQPVPWE TVYPGADRQA LSLLGRMLRF EPSARVSAAA ALRHPFLAKY
360 370 380 390 400
HDPDDEPDCA PPFDFAFDRE ALTRERIKEA IVAEIEDFHA RREGIRQQIR
410 420 430 440 450
FQPSLQPVAS EPGCPDVEMP SPWAPSGDCA MESPPPAPLP CPGPAPDTID
460 470 480 490 500
LTLQPPPPAS EPAPPKKEGA ISDNTKAALK AALLKSLRSR LRDGPSAPLE
510 520 530 540 550
APEPRKPVTA QERQREREEK RRRRQERAKE REKRRQERER KERGAGVSGG
560 570 580 590 600
PSADPLAGLV LSDNDRSLLE RWTRMAQPPA PAPATARPPS PPAGPATQPT
610 620 630 640 650
GPLPQPACPP PAPAAGPAAP QTTAASGLLA PQPLVPPPGL PGPSALSVLP
660 670 680 690 700
YFPSGPPPPD PGGAPQPSTS ESPDVTLVTQ QLSKSQVEDP LPPVFSGTPK
710 720 730 740 750
GSGAGYGVGF DLEEFLNQSF DMGVADGPQD GQADSASLSA SLLADWLEGH
760 770 780
GMNPADIESL QREIQMDSPM LLADLPDLQE P
Length:781
Mass (Da):84,832
Last modified:July 10, 2007 - v1
Checksum:i1BDFC98A7EA3751C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC142510 mRNA. Translation: AAI42511.1.
RefSeqiNP_001092550.1. NM_001099080.2.
XP_005220440.1. XM_005220383.1.
UniGeneiBt.103114.

Genome annotation databases

EnsembliENSBTAT00000001347; ENSBTAP00000001347; ENSBTAG00000001014.
GeneIDi537703.
KEGGibta:537703.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC142510 mRNA. Translation: AAI42511.1 .
RefSeqi NP_001092550.1. NM_001099080.2.
XP_005220440.1. XM_005220383.1.
UniGenei Bt.103114.

3D structure databases

ProteinModelPortali A5PKJ4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9913.ENSBTAP00000001347.

Proteomic databases

PRIDEi A5PKJ4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSBTAT00000001347 ; ENSBTAP00000001347 ; ENSBTAG00000001014 .
GeneIDi 537703.
KEGGi bta:537703.

Organism-specific databases

CTDi 5598.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00550000074298.
HOGENOMi HOG000113595.
HOVERGENi HBG108137.
InParanoidi A5PKJ4.
KOi K04464.
OMAi IIETIGT.
OrthoDBi EOG70PBX2.
TreeFami TF105099.

Enzyme and pathway databases

Reactomei REACT_203215. Senescence-Associated Secretory Phenotype (SASP).
REACT_205036. Gastrin-CREB signalling pathway via PKC and MAPK.
REACT_220398. ERKs are inactivated.
REACT_226850. ERK/MAPK targets.
REACT_227858. Signalling to ERK5.

Miscellaneous databases

NextBioi 20877197.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Fetal liver.

Entry informationi

Entry nameiMK07_BOVIN
AccessioniPrimary (citable) accession number: A5PKJ4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: July 10, 2007
Last modified: November 26, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3