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A5PKJ4

- MK07_BOVIN

UniProt

A5PKJ4 - MK07_BOVIN

Protein

Mitogen-activated protein kinase 7

Gene

MAPK7

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 63 (01 Oct 2014)
      Sequence version 1 (10 Jul 2007)
      Previous versions | rss
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    Functioni

    Plays a role in various cellular processes such as proliferation, differentiation and cell survival. The upstream activator of MAPK7 is the MAPK kinase MAP2K5. Upon activation, it translocates to the nucleus and phosphorylates various downstream targets including MEF2C. EGF activates MAPK7 through a Ras-independent and MAP2K5-dependent pathway. May have a role in muscle cell differentiation. May be important for endothelial function and maintenance of blood vessel integrity. MAP2K5 and MAPK7 interact specifically with one another and not with MEK1/ERK1 or MEK2/ERK2 pathways. Phosphorylates SGK1 at Ser-78 and this is required for growth factor-induced cell cycle progression By similarity. Involved in the regulation of p53/TP53 by disrupting the PML-MDM2 interaction By similarity.By similarity

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Cofactori

    Magnesium.By similarity

    Enzyme regulationi

    Activated by tyrosine and threonine phosphorylation. Activated in response to hyperosmolarity, hydrogen peroxide, and epidermal growth factor (EGF) By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei84 – 841ATPPROSITE-ProRule annotation
    Active sitei182 – 1821Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi61 – 699ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. MAP kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. cell cycle Source: UniProtKB-KW
    2. cell differentiation Source: UniProtKB-KW
    3. cellular response to hydrogen peroxide Source: Ensembl
    4. cellular response to transforming growth factor beta stimulus Source: Ensembl
    5. negative regulation of endothelial cell apoptotic process Source: Ensembl
    6. negative regulation of extrinsic apoptotic signaling pathway in absence of ligand Source: Ensembl
    7. negative regulation of heterotypic cell-cell adhesion Source: Ensembl
    8. negative regulation of NFAT protein import into nucleus Source: Ensembl
    9. negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway Source: Ensembl
    10. negative regulation of response to cytokine stimulus Source: Ensembl
    11. peptidyl-serine phosphorylation Source: Ensembl
    12. positive regulation of protein metabolic process Source: Ensembl
    13. positive regulation of transcription from RNA polymerase II promoter in response to stress Source: Ensembl
    14. regulation of angiogenesis Source: Ensembl

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Cell cycle, Differentiation

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_203215. Senescence-Associated Secretory Phenotype (SASP).
    REACT_205036. Gastrin-CREB signalling pathway via PKC and MAPK.
    REACT_220398. ERKs are inactivated.
    REACT_226850. ERK/MAPK targets.
    REACT_227858. Signalling to ERK5.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mitogen-activated protein kinase 7 (EC:2.7.11.24)
    Short name:
    MAP kinase 7
    Short name:
    MAPK 7
    Gene namesi
    Name:MAPK7
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 19

    Subcellular locationi

    Cytoplasm. Nucleus. NucleusPML body By similarity
    Note: Translocates to the nucleus upon activation.By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. cytosol Source: Ensembl
    3. nucleus Source: UniProtKB
    4. PML body Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 781780Mitogen-activated protein kinase 7PRO_0000349104Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineBy similarity
    Modified residuei685 – 6851PhosphoserineBy similarity
    Modified residuei698 – 6981PhosphothreonineBy similarity

    Post-translational modificationi

    Dually phosphorylated on Thr-219 and Tyr-221, which activates the enzyme.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PRIDEiA5PKJ4.

    Interactioni

    Subunit structurei

    Interacts with MAP2K5. Forms oligomers By similarity. Interacts with MEF2A, MEF2C and MEF2D; the interaction phosphorylates the MEF2s and enhances transcriptional activity of MEF2A, MEF2C but not MEF2D By similarity. Interacts with SGK1 By similarity. Interacts with PML By similarity.By similarity

    Protein-protein interaction databases

    STRINGi9913.ENSBTAP00000001347.

    Structurei

    3D structure databases

    ProteinModelPortaliA5PKJ4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini55 – 347293Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni2 – 7776Required for cytoplasmic targetingBy similarityAdd
    BLAST
    Regioni78 – 13962Required for binding to MAP2K5By similarityAdd
    BLAST
    Regioni140 – 406267Necessary for oligomerizationBy similarityAdd
    BLAST
    Regioni407 – 781375May not be required for kinase activity; required to stimulate MEF2C activityBy similarityAdd
    BLAST
    Regioni505 – 53935Nuclear localization signalBy similarityAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi219 – 2213TXYBy similarity

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi403 – 46563Pro-richAdd
    BLAST
    Compositional biasi513 – 54331Arg-richAdd
    BLAST
    Compositional biasi578 – 66790Pro-richAdd
    BLAST

    Domaini

    The second proline-rich region may interact with actin targeting the kinase to a specific location in the cell.
    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00550000074298.
    HOGENOMiHOG000113595.
    HOVERGENiHBG108137.
    InParanoidiA5PKJ4.
    KOiK04464.
    OMAiIIETIGT.
    OrthoDBiEOG70PBX2.
    TreeFamiTF105099.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR003527. MAP_kinase_CS.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS01351. MAPK. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    A5PKJ4-1 [UniParc]FASTAAdd to Basket

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    MAEPLKEDDG EDGSGEPPGP VKAEPAGTAA SVAAKNLALL KARSFDVTFD    50
    VGDEYEIIET IGNGAYGVVS SARRRLTGQQ VAIKKIPNAF DVVTNAKRTL 100
    RELKILKHFK HDNIIAIKDI LRPTVPYGEF KSVYVVLDLM ESDLHQIIHS 150
    SQPLTLEHVR YFLYQLLRGL KYMHSAQVIH RDLKPSNLLV NENCELKIGD 200
    FGMARGLCTS PAEHQYFMTE YVATRWYRAP ELMLSLHEYT QAIDLWSVGC 250
    IFGEMLARRQ LFPGKNYVHQ LQLIMTVLGT PSPAVIQAVG AERVRAYIQS 300
    LPPRQPVPWE TVYPGADRQA LSLLGRMLRF EPSARVSAAA ALRHPFLAKY 350
    HDPDDEPDCA PPFDFAFDRE ALTRERIKEA IVAEIEDFHA RREGIRQQIR 400
    FQPSLQPVAS EPGCPDVEMP SPWAPSGDCA MESPPPAPLP CPGPAPDTID 450
    LTLQPPPPAS EPAPPKKEGA ISDNTKAALK AALLKSLRSR LRDGPSAPLE 500
    APEPRKPVTA QERQREREEK RRRRQERAKE REKRRQERER KERGAGVSGG 550
    PSADPLAGLV LSDNDRSLLE RWTRMAQPPA PAPATARPPS PPAGPATQPT 600
    GPLPQPACPP PAPAAGPAAP QTTAASGLLA PQPLVPPPGL PGPSALSVLP 650
    YFPSGPPPPD PGGAPQPSTS ESPDVTLVTQ QLSKSQVEDP LPPVFSGTPK 700
    GSGAGYGVGF DLEEFLNQSF DMGVADGPQD GQADSASLSA SLLADWLEGH 750
    GMNPADIESL QREIQMDSPM LLADLPDLQE P 781
    Length:781
    Mass (Da):84,832
    Last modified:July 10, 2007 - v1
    Checksum:i1BDFC98A7EA3751C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC142510 mRNA. Translation: AAI42511.1.
    RefSeqiNP_001092550.1. NM_001099080.2.
    XP_005220440.1. XM_005220383.1.
    UniGeneiBt.103114.

    Genome annotation databases

    EnsembliENSBTAT00000001347; ENSBTAP00000001347; ENSBTAG00000001014.
    GeneIDi537703.
    KEGGibta:537703.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC142510 mRNA. Translation: AAI42511.1 .
    RefSeqi NP_001092550.1. NM_001099080.2.
    XP_005220440.1. XM_005220383.1.
    UniGenei Bt.103114.

    3D structure databases

    ProteinModelPortali A5PKJ4.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9913.ENSBTAP00000001347.

    Proteomic databases

    PRIDEi A5PKJ4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000001347 ; ENSBTAP00000001347 ; ENSBTAG00000001014 .
    GeneIDi 537703.
    KEGGi bta:537703.

    Organism-specific databases

    CTDi 5598.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00550000074298.
    HOGENOMi HOG000113595.
    HOVERGENi HBG108137.
    InParanoidi A5PKJ4.
    KOi K04464.
    OMAi IIETIGT.
    OrthoDBi EOG70PBX2.
    TreeFami TF105099.

    Enzyme and pathway databases

    Reactomei REACT_203215. Senescence-Associated Secretory Phenotype (SASP).
    REACT_205036. Gastrin-CREB signalling pathway via PKC and MAPK.
    REACT_220398. ERKs are inactivated.
    REACT_226850. ERK/MAPK targets.
    REACT_227858. Signalling to ERK5.

    Miscellaneous databases

    NextBioi 20877197.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR003527. MAP_kinase_CS.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS01351. MAPK. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. NIH - Mammalian Gene Collection (MGC) project
      Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Hereford.
      Tissue: Fetal liver.

    Entry informationi

    Entry nameiMK07_BOVIN
    AccessioniPrimary (citable) accession number: A5PKJ4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 2, 2008
    Last sequence update: July 10, 2007
    Last modified: October 1, 2014
    This is version 63 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3