Reviewed, UniProtKB/Swiss-Prot A5PKJ4 (MK07_BOVIN)

Names and origin

Protein names

Recommended name:
    Mitogen-activated protein kinase 7
    EC=2.7.11.24
Alternative name(s):
    Extracellular signal-regulated kinase 5
      Short name=ERK-5
    Big MAP kinase 1
      Short name=BMK1 kinase

Gene names

Name:

MAPK7

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	781 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Plays a role in various cellular processes such as proliferation, differentiation and cell survival. The upstream activator of MAPK7 is the MAPK kinase MAP2K5. Upon activation, it translocates to the nucleus and phosphorylates various downstream targets including MEF2C. EGF activates MAPK7 through a Ras-independent and MAP2K5-dependent pathway. May have a role in muscle cell differentiation. May be important for endothelial function and maintenance of blood vessel integrity. MAP2K5 and MAPK7 interact specifically with one another and not with MEK1/ERK1 or MEK2/ERK2 pathways By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Cofactor	Magnesium By similarity.
Enzyme regulation	Activated by tyrosine and threonine phosphorylation. Activated in response to hyperosmolarity, hydrogen peroxide, and epidermal growth factor (EGF) By similarity.
Subunit structure	Interacts with MAP2K5. Forms oligomers By similarity. Interacts with MEF2A, MEF2C and MEF2D; the interaction phosphorylates the MEF2s and enhances transcriptional activity of MEF2A, MEF2C but not MEF2D By similarity.
Subcellular location	Cytoplasm. Nucleus. Note: Translocates to the nucleus upon activation By similarity.
Domain	The second proline-rich region may interact with actin targeting the kinase to a specific location in the cell. The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
Post-translational modification	Dually phosphorylated on Thr-219 and Tyr-221, which activates the enzyme By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Cell cycle Differentiation
Cellular component	Cytoplasm Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW MAP kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 781	781	Mitogen-activated protein kinase 7		PRO_0000349104
Regions
	Domain	55 – 347	293	Protein kinase
	Nucleotide binding	61 – 69	9	ATP By similarity
	Region	1 – 77	77	Required for cytoplasmic targeting By similarity
	Region	78 – 139	62	Required for binding to MAP2K5 By similarity
	Region	140 – 406	267	Necessary for oligomerization By similarity
	Region	407 – 781	375	May not be required for kinase activity; required to stimulate MEF2C activity By similarity
	Region	505 – 539	35	Nuclear localization signal By similarity
	Motif	219 – 221	3	TXY By similarity
	Compositional bias	403 – 465	63	Pro-rich
	Compositional bias	513 – 543	31	Arg-rich
	Compositional bias	578 – 667	90	Pro-rich
Sites
	Active site	182	1	Proton acceptor By similarity
	Binding site	84	1	ATP By similarity
Amino acid modifications
	Modified residue	219	1	Phosphothreonine By similarity
	Modified residue	221	1	Phosphotyrosine By similarity
	Modified residue	685	1	Phosphoserine By similarity
	Modified residue	698	1	Phosphothreonine By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5PKJ4-1 [UniParc]. Last modified July 10, 2007. Version 1. Checksum: 1BDFC98A7EA3751C	FASTA	781	84,832
	10 20 30 40 50 60 MAEPLKEDDG EDGSGEPPGP VKAEPAGTAA SVAAKNLALL KARSFDVTFD VGDEYEIIET 70 80 90 100 110 120 IGNGAYGVVS SARRRLTGQQ VAIKKIPNAF DVVTNAKRTL RELKILKHFK HDNIIAIKDI 130 140 150 160 170 180 LRPTVPYGEF KSVYVVLDLM ESDLHQIIHS SQPLTLEHVR YFLYQLLRGL KYMHSAQVIH 190 200 210 220 230 240 RDLKPSNLLV NENCELKIGD FGMARGLCTS PAEHQYFMTE YVATRWYRAP ELMLSLHEYT 250 260 270 280 290 300 QAIDLWSVGC IFGEMLARRQ LFPGKNYVHQ LQLIMTVLGT PSPAVIQAVG AERVRAYIQS 310 320 330 340 350 360 LPPRQPVPWE TVYPGADRQA LSLLGRMLRF EPSARVSAAA ALRHPFLAKY HDPDDEPDCA 370 380 390 400 410 420 PPFDFAFDRE ALTRERIKEA IVAEIEDFHA RREGIRQQIR FQPSLQPVAS EPGCPDVEMP 430 440 450 460 470 480 SPWAPSGDCA MESPPPAPLP CPGPAPDTID LTLQPPPPAS EPAPPKKEGA ISDNTKAALK 490 500 510 520 530 540 AALLKSLRSR LRDGPSAPLE APEPRKPVTA QERQREREEK RRRRQERAKE REKRRQERER 550 560 570 580 590 600 KERGAGVSGG PSADPLAGLV LSDNDRSLLE RWTRMAQPPA PAPATARPPS PPAGPATQPT 610 620 630 640 650 660 GPLPQPACPP PAPAAGPAAP QTTAASGLLA PQPLVPPPGL PGPSALSVLP YFPSGPPPPD 670 680 690 700 710 720 PGGAPQPSTS ESPDVTLVTQ QLSKSQVEDP LPPVFSGTPK GSGAGYGVGF DLEEFLNQSF 730 740 750 760 770 780 DMGVADGPQD GQADSASLSA SLLADWLEGH GMNPADIESL QREIQMDSPM LLADLPDLQE P

References

[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Fetal liver.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC142510 mRNA. Translation: AAI42511.1.
IPI	IPI00854534.
RefSeq	NP_001092550.1.
UniGene	Bt.5155
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000001347; ENSBTAP00000001347; ENSBTAG00000001014; Bos taurus. [Genome view]
GeneID	537703.
KEGG	bta:537703.
Organism-specific databases
CTD	537703.
Family and domain databases
InterPro	IPR003527. MAP_kinase_CS. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS01351. MAPK. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MK07_BOVIN

Accession

Primary (citable) accession number: A5PKJ4

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	July 10, 2007
Last modified:	October 13, 2009
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)