Reviewed, UniProtKB/Swiss-Prot A5PK51 (PNCB_BOVIN)

Names and origin

Protein names

Recommended name:
    Nicotinate phosphoribosyltransferase
      Short name=NAPRTase
    EC=2.4.2.11
Alternative name(s):
    Nicotinate phosphoribosyltransferase domain-containing protein 1

Gene names

Name:

NAPRT1

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	538 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the conversion of nicotinic acid (NA) to NA mononucleotide (NaMN). Essential for NA to increase cellular NAD levels and prevent oxidative stress of the cells By similarity.
Catalytic activity	Nicotinate D-ribonucleotide + diphosphate = nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate.
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; nicotinate D-ribonucleotide from nicotinate: step 1/1.
Subcellular location	Cytoplasm › cytosol By similarity.
Sequence similarities	Belongs to the NAPRTase family.

Ontologies

Keywords
Biological process	Pyridine nucleotide biosynthesis
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Molecular function	Glycosyltransferase Transferase
Gene Ontology (GO)
Biological process	pyridine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytosol Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nicotinate phosphoribosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: A5PK51-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: A5PK51-2)

The sequence of this isoform differs from the canonical sequence as follows:
     431-435: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 538	538	Nicotinate phosphoribosyltransferase		PRO_0000315680
Natural variations
	Alternative sequence	431 – 435	5	Missing in isoform 2.		VSP_030609
Experimental info
	Sequence conflict	58	1	A → E in AAP69606. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified January 15, 2008. Version 2. Checksum: CDE2E6FF34A613A9	FASTA	538	57,871
	10 20 30 40 50 60 MAAEQDPEGR AAARPLLTDL YQATMALGYW RAGRAQDQAE FELFFRQCPF GGAFALAAGL 70 80 90 100 110 120 RDCVRFLRAF RLRDADVQFL ASALPPDTDP AFFEHLRALD CSGVTVRALP EGSLAFPGVP 130 140 150 160 170 180 LLQVSGPLLV VQLLETPLLC LVSYASLIAT NAARLRLIAG PDKRLLEMGL RRAQGPDGGL 190 200 210 220 230 240 TASTYSYLGG FDASSNVLAG QLRGVPVAGT LAHSFVTSFS GSEVPPDPML APAAGQGSQV 250 260 270 280 290 300 DLAASVEMWL ERVCGHLGLG VQEPHRGERA AFVAYALAFP RAFQGLLDTY SVRRSGLPNF 310 320 330 340 350 360 LAVALALQEL GYQAVGVRLD SGDLLQQARE IRGVFRTAAA QFGVPWLQSV PIAVSNNIDE 370 380 390 400 410 420 EELARLAQKG SEVNVIGIGT SVVTCPRQPS LGCVYKLVSV GGQPRMKLTE DPEKQTLPGS 430 440 450 460 470 480 KAAFRLLGSD GSLLLDVLQL AEEPPPQAGQ ELRVWPRGAR ESRTVRPAHV EPLLRLWVQQ 490 500 510 520 530 GQLCEPLPSL AESRAFAQQS LHRLSPAHRR LEQPALYQVA LSEKLQALVD RLSARGAL
	Isoform 2. Checksum: 1DE470E605E4D07F	FASTA	533	57,388
	10 20 30 40 50 60 MAAEQDPEGR AAARPLLTDL YQATMALGYW RAGRAQDQAE FELFFRQCPF GGAFALAAGL 70 80 90 100 110 120 RDCVRFLRAF RLRDADVQFL ASALPPDTDP AFFEHLRALD CSGVTVRALP EGSLAFPGVP 130 140 150 160 170 180 LLQVSGPLLV VQLLETPLLC LVSYASLIAT NAARLRLIAG PDKRLLEMGL RRAQGPDGGL 190 200 210 220 230 240 TASTYSYLGG FDASSNVLAG QLRGVPVAGT LAHSFVTSFS GSEVPPDPML APAAGQGSQV 250 260 270 280 290 300 DLAASVEMWL ERVCGHLGLG VQEPHRGERA AFVAYALAFP RAFQGLLDTY SVRRSGLPNF 310 320 330 340 350 360 LAVALALQEL GYQAVGVRLD SGDLLQQARE IRGVFRTAAA QFGVPWLQSV PIAVSNNIDE 370 380 390 400 410 420 EELARLAQKG SEVNVIGIGT SVVTCPRQPS LGCVYKLVSV GGQPRMKLTE DPEKQTLPGS 430 440 450 460 470 480 KAAFRLLGSD DVLQLAEEPP PQAGQELRVW PRGARESRTV RPAHVEPLLR LWVQQGQLCE 490 500 510 520 530 PLPSLAESRA FAQQSLHRLS PAHRRLEQPA LYQVALSEKL QALVDRLSAR GAL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: Crossbred X Angus. Tissue: Ileum.
[2]	"Sequence analysis and molecular evolution of the eukaryotic nicotinate phosphoribosyltranferase family." Huang C.-H., Peng J., Chen Y. Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 17-538 (ISOFORM 1).

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC142357 mRNA. Translation: AAI42358.1. AY214328 mRNA. Translation: AAP69606.1.
IPI	IPI00854386. IPI00883387.
RefSeq	NP_001092327.1.
UniGene	Bt.23360
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A5PK51.
Genome annotation databases
Ensembl	ENSBTAT00000019496; ENSBTAP00000019496; ENSBTAG00000014642; Bos taurus. [Genome view]
GeneID	399559.
KEGG	bta:399559.
Organism-specific databases
CTD	399559.
Enzyme and pathway databases
BRENDA	2.4.2.11. 251.
Family and domain databases
InterPro	IPR015977. Nic_PRibTrfase-like. IPR006405. Nic_PRibTrfase_put. [Graphical view]
Pfam	PF04095. NAPRTase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01513. NAPRTase_put. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PNCB_BOVIN

Accession

Primary (citable) accession number: A5PK51
Secondary accession number(s): Q6XQN3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	January 15, 2008
Last modified:	September 22, 2009
This is version 21 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)