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A5PK51 (PNCB_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 52. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nicotinate phosphoribosyltransferase

Short name=NAPRTase
EC=6.3.4.21
Alternative name(s):
Nicotinate phosphoribosyltransferase domain-containing protein 1
Gene names
Name:NAPRT1
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length538 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the conversion of nicotinic acid (NA) to NA mononucleotide (NaMN). Essential for NA to increase cellular NAD levels and prevent oxidative stress of the cells By similarity.

Catalyzes the synthesis of beta-nicotinate D-ribonucleotide from nicotinate and 5-phospho-D-ribose 1-phosphate at the expense of ATP By similarity.

Catalytic activity

Nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O = beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate.

Pathway

Cofactor biosynthesis; NAD(+) biosynthesis; nicotinate D-ribonucleotide from nicotinate: step 1/1.

Subcellular location

Cytoplasmcytosol By similarity.

Sequence similarities

Belongs to the NAPRTase family.

Ontologies

Keywords
   Biological processPyridine nucleotide biosynthesis
   Cellular componentCytoplasm
   Coding sequence diversityAlternative splicing
   Molecular functionLigase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processNAD biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

nicotinate nucleotide salvage

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytosol

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionligase activity

Inferred from electronic annotation. Source: UniProtKB-KW

nicotinate phosphoribosyltransferase activity

Inferred from electronic annotation. Source: InterPro

nicotinate-nucleotide diphosphorylase (carboxylating) activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: A5PK51-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: A5PK51-2)

The sequence of this isoform differs from the canonical sequence as follows:
     431-435: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 538538Nicotinate phosphoribosyltransferase
PRO_0000315680

Natural variations

Alternative sequence431 – 4355Missing in isoform 2.
VSP_030609

Experimental info

Sequence conflict581A → E in AAP69606. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified January 15, 2008. Version 2.
Checksum: CDE2E6FF34A613A9

FASTA53857,871
        10         20         30         40         50         60 
MAAEQDPEGR AAARPLLTDL YQATMALGYW RAGRAQDQAE FELFFRQCPF GGAFALAAGL 

        70         80         90        100        110        120 
RDCVRFLRAF RLRDADVQFL ASALPPDTDP AFFEHLRALD CSGVTVRALP EGSLAFPGVP 

       130        140        150        160        170        180 
LLQVSGPLLV VQLLETPLLC LVSYASLIAT NAARLRLIAG PDKRLLEMGL RRAQGPDGGL 

       190        200        210        220        230        240 
TASTYSYLGG FDASSNVLAG QLRGVPVAGT LAHSFVTSFS GSEVPPDPML APAAGQGSQV 

       250        260        270        280        290        300 
DLAASVEMWL ERVCGHLGLG VQEPHRGERA AFVAYALAFP RAFQGLLDTY SVRRSGLPNF 

       310        320        330        340        350        360 
LAVALALQEL GYQAVGVRLD SGDLLQQARE IRGVFRTAAA QFGVPWLQSV PIAVSNNIDE 

       370        380        390        400        410        420 
EELARLAQKG SEVNVIGIGT SVVTCPRQPS LGCVYKLVSV GGQPRMKLTE DPEKQTLPGS 

       430        440        450        460        470        480 
KAAFRLLGSD GSLLLDVLQL AEEPPPQAGQ ELRVWPRGAR ESRTVRPAHV EPLLRLWVQQ 

       490        500        510        520        530 
GQLCEPLPSL AESRAFAQQS LHRLSPAHRR LEQPALYQVA LSEKLQALVD RLSARGAL 

« Hide

Isoform 2 [UniParc].

Checksum: 1DE470E605E4D07F
Show »

FASTA53357,388

References

« Hide 'large scale' references
[1]NIH - Mammalian Gene Collection (MGC) project
Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: Crossbred X Angus.
Tissue: Ileum.
[2]"Sequence analysis and molecular evolution of the eukaryotic nicotinate phosphoribosyltransferase family."
Huang C.-H., Peng J., Chen Y.
Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 17-538 (ISOFORM 1).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC142357 mRNA. Translation: AAI42358.1.
AY214328 mRNA. Translation: AAP69606.1.
RefSeqNP_001092327.1. NM_001098857.2. [A5PK51-2]
UniGeneBt.23360.

3D structure databases

ProteinModelPortalA5PK51.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEA5PK51.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID399559.
KEGGbta:399559.

Organism-specific databases

CTD93100.

Phylogenomic databases

eggNOGCOG1488.
HOGENOMHOG000001111.
HOVERGENHBG062439.
InParanoidA5PK51.
KOK00763.

Enzyme and pathway databases

UniPathwayUPA00253; UER00457.

Family and domain databases

InterProIPR007229. Nic_PRibTrfase-Fam.
IPR006405. Nic_PRibTrfase_pncB.
IPR002638. Quinolinate_PRibosylTrfase_C.
[Graphical view]
PANTHERPTHR11098:SF1. PTHR11098:SF1. 1 hit.
PfamPF04095. NAPRTase. 2 hits.
[Graphical view]
PIRSFPIRSF000484. NAPRT. 1 hit.
SUPFAMSSF51690. SSF51690. 2 hits.
TIGRFAMsTIGR01513. NAPRTase_put. 1 hit.
ProtoNetSearch...

Other

NextBio20816605.

Entry information

Entry namePNCB_BOVIN
AccessionPrimary (citable) accession number: A5PK51
Secondary accession number(s): Q6XQN3
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 15, 2008
Last modified: June 11, 2014
This is version 52 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways