A5PK45 (GT251_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Procollagen galactosyltransferase 1
EC=2.4.1.50

Alternative name(s):
Collagen beta(1-O)galactosyltransferase 1
Glycosyltransferase 25 family member 1
Hydroxylysine galactosyltransferase 1

Gene names

Name:	COLGALT1
Synonyms:	GLT25D1

Organism

Bos taurus (Bovine) [Reference proteome]

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	623 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Has a beta-galactosyltransferase activity; transfers beta-galactose to hydroxylysine residues of collagen By similarity.
Catalytic activity	UDP-alpha-D-galactose + 5-hydroxy-L-lysine-[procollagen] = UDP + 5-(D-galactosyloxy)-L-lysine-[procollagen].
Subcellular location	Endoplasmic reticulum lumen By similarity. Note: Colocalized with PLOD3 and mannose binding lectin By similarity.
Post-translational modification	N-glycosylated By similarity.
Sequence similarities	Belongs to the glycosyltransferase 25 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum
Domain	Signal
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	lipopolysaccharide biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular_component	endoplasmic reticulum lumen Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	procollagen galactosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 30	30	Potential
	Chain	31 – 623	593	Procollagen galactosyltransferase 1		PRO_0000309535
Regions
	Motif	620 – 623	4	Prevents secretion from ER By similarity
Amino acid modifications
	Glycosylation	97	1	N-linked (GlcNAc...) Potential
	Glycosylation	185	1	N-linked (GlcNAc...) Potential
	Glycosylation	382	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	A5PK45 [UniParc]. Last modified July 10, 2007. Version 1. Checksum: 8DE0E6A8A66A8384	FASTA	623	71,547
	10 20 30 40 50 60 MAAAPRACKG HGRPLPVLLL LLLLALPPLG GPPGAAAYFP EERWSPESPL QAPRVLIALL 70 80 90 100 110 120 ARNAAHALPA TLGALERLRH PRERTALWVA TDHNADNTSA VLREWLVAVK GLYHSVEWRP 130 140 150 160 170 180 SEEPRSYPDE EGPKHWSDSR YEHVMKLRQA ALKSARDMWA DYILFVDADN LILNPDTLTL 190 200 210 220 230 240 LIAENKTVVA PMLDSRAAYS NFWCGMTSQG YYKRTPAYIP IRKRERRGCF AVPMVHSTFL 250 260 270 280 290 300 IDLRKAASRN LAFYPPHPDY TWSFDDIIVF AFSCKQAEVQ MYVCNKEVYG FLPVPLRSHS 310 320 330 340 350 360 TLQDEAESFM HVQLEVMVKH PPSEPSRFIS VPTKTPDKMG FDEVFMINLK RRQDRRERML 370 380 390 400 410 420 RALEEQEIAC RLVEAVDGKA MNTSQVEALG IQMLPGYRDP YHGRPLTKGE LGCFLSHYNI 430 440 450 460 470 480 WKEVVDRGLQ KSLVFEDDLR FEIFFKRRLM NLMQDVEREG LDWDLIYVGR KRMQVEHPEK 490 500 510 520 530 540 AVPRVRNLVE ADYSYWTLAY VISLQGARKL LAARPLSKML PVDEFLPVMF DKHPVSEYKA 550 560 570 580 590 600 HFSPRDLRAF SVEPLLIYPT HYTGDDGYVS DTETSVVWNN EHVKTDWDRA KSQKMREQQA 610 620 LSREAKNSDV LQSPLDSAAR DEL

References

[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Fetal pancreas.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC142350 mRNA. Translation: AAI42351.1.
IPI	IPI00713107.
RefSeq	NP_001092425.1. NM_001098955.1.
UniGene	Bt.29548.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	9913.ENSBTAP00000016841.
Protein family/group databases
CAZy	GT25. Glycosyltransferase Family 25.
Proteomic databases
PRIDE	A5PK45.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000016841; ENSBTAP00000016841; ENSBTAG00000012678.
GeneID	513167.
KEGG	bta:513167.
Organism-specific databases
CTD	79709.
Phylogenomic databases
eggNOG	COG3306.
GeneTree	ENSGT00550000074427.
HOGENOM	HOG000007198.
HOVERGEN	HBG058097.
InParanoid	A5PK45.
KO	K11703.
OMA	QLEVMVK.
OrthoDB	EOG4B2SWX.
Family and domain databases
InterPro	IPR002654. Glyco_trans_25. [Graphical view]
Pfam	PF01755. Glyco_transf_25. 1 hit. [Graphical view]
PROSITE	PS00014. ER_TARGET. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20870734.

Entry information

Entry name

GT251_BOVIN

Accession

Primary (citable) accession number: A5PK45

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 13, 2007
Last sequence update:	July 10, 2007
Last modified:	May 1, 2013
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program