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Protein

Cyclin-F

Gene

CCNF

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of CP110 during G2 phase, thereby acting as an inhibitor of centrosome reduplication.By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Cyclin

Keywords - Biological processi

Cell cycle, Cell division, Mitosis, Ubl conjugation pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclin-F
Gene namesi
Name:CCNF
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136 Componenti: Chromosome 25

Subcellular locationi

  • Nucleus By similarity
  • Cytoplasmcytoskeletonmicrotubule organizing centercentrosomecentriole By similarity

  • Note: Localization in the centrosome is rare in S phase cells and increases in G2 cells, Localizes on both the mother and daughter centrioles. Localization to centrosomes is not dependent on CP110. Also localizes to the nucleus (By similarity).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 788788Cyclin-FPRO_0000398634Add
BLAST

Post-translational modificationi

Degraded when the spindle assembly checkpoint is activated during the G2-M transition. Degradation is not dependent on the proteasome or ubiquitin and depends on the C-terminal PEST sequence (By similarity).By similarity
Phosphorylated just before cells enter into mitosis.By similarity

Keywords - PTMi

Phosphoprotein

Expressioni

Gene expression databases

ExpressionAtlasiA5PK16. baseline.

Interactioni

Subunit structurei

Component of the SCF(CCNF) complex consisting of CUL1, RBX1, SKP1 and CCNF. Interacts with CCNB1; interaction is required for nuclear localization of CCNB1. Interacts with CCP110; this interaction leads to CCP110 ubiquitination and degradation via the proteasome pathway (By similarity).By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000034530.

Structurei

3D structure databases

ProteinModelPortaliA5PK16.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini29 – 7648F-boxPROSITE-ProRule annotationAdd
BLAST
Domaini294 – 405112Cyclin N-terminalAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni582 – 766185PESTAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi20 – 289Nuclear localization signal 1By similarity
Motifi568 – 5747Nuclear localization signal 2By similarity

Domaini

The nuclear localization signals mediate the localization to the nucleus and are required for CCNB1 localization to the nucleus.By similarity

Sequence similaritiesi

Belongs to the cyclin family. Cyclin AB subfamily.Curated
Contains 1 cyclin N-terminal domain.Curated
Contains 1 F-box domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG283043.
GeneTreeiENSGT00760000118939.
HOGENOMiHOG000285971.
HOVERGENiHBG050838.
InParanoidiA5PK16.
KOiK10289.
OMAiLHQKCFH.
OrthoDBiEOG7WDN1V.
TreeFamiTF101006.

Family and domain databases

Gene3Di1.10.472.10. 2 hits.
1.25.40.10. 2 hits.
InterProiIPR028857. CCNF_metazoan.
IPR013763. Cyclin-like.
IPR004367. Cyclin_C-dom.
IPR006671. Cyclin_N.
IPR001810. F-box_dom.
IPR011990. TPR-like_helical_dom.
[Graphical view]
PANTHERiPTHR10177:SF183. PTHR10177:SF183. 1 hit.
PfamiPF02984. Cyclin_C. 1 hit.
PF00134. Cyclin_N. 1 hit.
PF00646. F-box. 1 hit.
[Graphical view]
SMARTiSM00385. CYCLIN. 2 hits.
SM00256. FBOX. 1 hit.
[Graphical view]
SUPFAMiSSF47954. SSF47954. 2 hits.
SSF81383. SSF81383. 1 hit.
PROSITEiPS00292. CYCLINS. 1 hit.
PS50181. FBOX. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A5PK16-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSGGVIHCR CAKCFCYPSK RRIRRRPRNL TILNLPEDAL FHILKWLSVG
60 70 80 90 100
DILAVRAVHS HLKYLVDNHA SVWACASFQE LWPSPGNLKL FERAAEKGNF
110 120 130 140 150
EAAVKLGIAY LYNEGLSVSD EARAEVNGLR ASRYFSLAER LNVGAAPFIW
160 170 180 190 200
LFIRPPWSVS GSCCKAVVHE SLRAECQLQK THRASILHCL GRVLSLFEDE
210 220 230 240 250
EKQKQARKLF EESANQGCLT SSYLLWESDR RMDMLDPGRC LHSFRKLRDF
260 270 280 290 300
AAKGCWEAQL SLAKACAHGH QLGLEAKASS EIVCQLFQAS HAVNKQRVFS
310 320 330 340 350
VQKGLNDTMR YILIDWLVEV ATMKDFSSLC LHLTVECVDR YLRRRLVPRY
360 370 380 390 400
RLQLLGIACM VICTRFISKE ILTIREAVWL TDNTYKYEDL VRMMGEVVSA
410 420 430 440 450
LDGKIRVPTV VDYKDVLLTL VPMAPRTQHL CSFLCELSLL HTSLAAYAPA
460 470 480 490 500
HLAAAALLLA RLTHGQTQPW TTQLWDLTGF SCEDLIPCVL SLHQKCFHDD
510 520 530 540 550
APKDYRQVSL TAVKQRFEDK RYEEISLEEV LSYGQLCAAL GVKQESLEPA
560 570 580 590 600
PFLSAGDIHA FLSSPSARRT KRKRENSLQE DRGSFVTTPT AELSSQEETL
610 620 630 640 650
LGSFLDWSLD YCSGYEGDQE SEGEKEGDVT APSGVLDVTV VYLSPEEHCC
660 670 680 690 700
QESSDEEACP EEACGAQDTQ ALVPGHQALR TPGPEPPLCS RWGLGKDVTT
710 720 730 740 750
SGYSSVNSAS PTDSGRTSGG PPRSTSELPT GSSLNTQPCH HHARKSCLQC
760 770 780
RPPSPPESCA PQQQVKRKNL SAHSEEEEED MNLGFLKL
Length:788
Mass (Da):87,898
Last modified:July 10, 2007 - v1
Checksum:i44621CC1B81BC5CE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC142318 mRNA. Translation: AAI42319.1.
RefSeqiNP_001092340.1. NM_001098870.2.
UniGeneiBt.43142.

Genome annotation databases

EnsembliENSBTAT00000034643; ENSBTAP00000034530; ENSBTAG00000006591.
GeneIDi505200.
KEGGibta:505200.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC142318 mRNA. Translation: AAI42319.1.
RefSeqiNP_001092340.1. NM_001098870.2.
UniGeneiBt.43142.

3D structure databases

ProteinModelPortaliA5PK16.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000034530.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000034643; ENSBTAP00000034530; ENSBTAG00000006591.
GeneIDi505200.
KEGGibta:505200.

Organism-specific databases

CTDi899.

Phylogenomic databases

eggNOGiNOG283043.
GeneTreeiENSGT00760000118939.
HOGENOMiHOG000285971.
HOVERGENiHBG050838.
InParanoidiA5PK16.
KOiK10289.
OMAiLHQKCFH.
OrthoDBiEOG7WDN1V.
TreeFamiTF101006.

Miscellaneous databases

NextBioi20867028.

Gene expression databases

ExpressionAtlasiA5PK16. baseline.

Family and domain databases

Gene3Di1.10.472.10. 2 hits.
1.25.40.10. 2 hits.
InterProiIPR028857. CCNF_metazoan.
IPR013763. Cyclin-like.
IPR004367. Cyclin_C-dom.
IPR006671. Cyclin_N.
IPR001810. F-box_dom.
IPR011990. TPR-like_helical_dom.
[Graphical view]
PANTHERiPTHR10177:SF183. PTHR10177:SF183. 1 hit.
PfamiPF02984. Cyclin_C. 1 hit.
PF00134. Cyclin_N. 1 hit.
PF00646. F-box. 1 hit.
[Graphical view]
SMARTiSM00385. CYCLIN. 2 hits.
SM00256. FBOX. 1 hit.
[Graphical view]
SUPFAMiSSF47954. SSF47954. 2 hits.
SSF81383. SSF81383. 1 hit.
PROSITEiPS00292. CYCLINS. 1 hit.
PS50181. FBOX. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Thymus.

Entry informationi

Entry nameiCCNF_BOVIN
AccessioniPrimary (citable) accession number: A5PK16
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: July 10, 2007
Last modified: July 22, 2015
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.