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A5PK16 (CCNF_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cyclin-F
Gene names
Name:CCNF
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length788 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of CP110 during G2 phase, thereby acting as an inhibitor of centrosome reduplication By similarity.

Subunit structure

Component of the SCF(CCNF) complex consisting of CUL1, RBX1, SKP1 and CCNF. Interacts with CCNB1; interaction is required for nuclear localization of CCNB1. Interacts with CCP110; this interaction leads to CCP110 ubiquitination and degradation via the proteasome pathway By similarity.

Subcellular location

Nucleus By similarity. Cytoplasmcytoskeletonmicrotubule organizing centercentrosomecentriole By similarity. Note: Localization in the centrosome is rare in S phase cells and increases in G2 cells, Localizes on both the mother and daughter centrioles. Localization to centrosomes is not dependent on CP110. Also localizes to the nucleus By similarity.

Domain

The nuclear localization signals mediate the localization to the nucleus and are required for CCNB1 localization to the nucleus By similarity.

Post-translational modification

Degraded when the spindle assembly checkpoint is activated during the G2-M transition. Degradation is not dependent on the proteasome or ubiquitin and depends on the C-terminal PEST sequence By similarity.

Phosphorylated just before cells enter into mitosis By similarity.

Sequence similarities

Belongs to the cyclin family. Cyclin AB subfamily.

Contains 1 cyclin N-terminal domain.

Contains 1 F-box domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 788788Cyclin-F
PRO_0000398634

Regions

Domain29 – 7648F-box
Domain294 – 405112Cyclin N-terminal
Region582 – 766185PEST
Motif20 – 289Nuclear localization signal 1 By similarity
Motif568 – 5747Nuclear localization signal 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
A5PK16 [UniParc].

Last modified July 10, 2007. Version 1.
Checksum: 44621CC1B81BC5CE

FASTA78887,898
        10         20         30         40         50         60 
MGSGGVIHCR CAKCFCYPSK RRIRRRPRNL TILNLPEDAL FHILKWLSVG DILAVRAVHS 

        70         80         90        100        110        120 
HLKYLVDNHA SVWACASFQE LWPSPGNLKL FERAAEKGNF EAAVKLGIAY LYNEGLSVSD 

       130        140        150        160        170        180 
EARAEVNGLR ASRYFSLAER LNVGAAPFIW LFIRPPWSVS GSCCKAVVHE SLRAECQLQK 

       190        200        210        220        230        240 
THRASILHCL GRVLSLFEDE EKQKQARKLF EESANQGCLT SSYLLWESDR RMDMLDPGRC 

       250        260        270        280        290        300 
LHSFRKLRDF AAKGCWEAQL SLAKACAHGH QLGLEAKASS EIVCQLFQAS HAVNKQRVFS 

       310        320        330        340        350        360 
VQKGLNDTMR YILIDWLVEV ATMKDFSSLC LHLTVECVDR YLRRRLVPRY RLQLLGIACM 

       370        380        390        400        410        420 
VICTRFISKE ILTIREAVWL TDNTYKYEDL VRMMGEVVSA LDGKIRVPTV VDYKDVLLTL 

       430        440        450        460        470        480 
VPMAPRTQHL CSFLCELSLL HTSLAAYAPA HLAAAALLLA RLTHGQTQPW TTQLWDLTGF 

       490        500        510        520        530        540 
SCEDLIPCVL SLHQKCFHDD APKDYRQVSL TAVKQRFEDK RYEEISLEEV LSYGQLCAAL 

       550        560        570        580        590        600 
GVKQESLEPA PFLSAGDIHA FLSSPSARRT KRKRENSLQE DRGSFVTTPT AELSSQEETL 

       610        620        630        640        650        660 
LGSFLDWSLD YCSGYEGDQE SEGEKEGDVT APSGVLDVTV VYLSPEEHCC QESSDEEACP 

       670        680        690        700        710        720 
EEACGAQDTQ ALVPGHQALR TPGPEPPLCS RWGLGKDVTT SGYSSVNSAS PTDSGRTSGG 

       730        740        750        760        770        780 
PPRSTSELPT GSSLNTQPCH HHARKSCLQC RPPSPPESCA PQQQVKRKNL SAHSEEEEED 


MNLGFLKL 

« Hide

References

[1]NIH - Mammalian Gene Collection (MGC) project
Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Hereford.
Tissue: Thymus.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC142318 mRNA. Translation: AAI42319.1.
RefSeqNP_001092340.1. NM_001098870.2.
UniGeneBt.43142.

3D structure databases

ProteinModelPortalA5PK16.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9913.ENSBTAP00000034530.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSBTAT00000034643; ENSBTAP00000034530; ENSBTAG00000006591.
GeneID505200.
KEGGbta:505200.

Organism-specific databases

CTD899.

Phylogenomic databases

eggNOGNOG283043.
HOGENOMHOG000285971.
HOVERGENHBG050838.
InParanoidA5PK16.
KOK10289.
OMAKSCLQCR.
OrthoDBEOG7WDN1V.
TreeFamTF101006.

Family and domain databases

Gene3D1.10.472.10. 2 hits.
1.25.40.10. 2 hits.
InterProIPR028857. CCNF_metazoan.
IPR013763. Cyclin-like.
IPR004367. Cyclin_C-dom.
IPR006671. Cyclin_N.
IPR001810. F-box_dom.
IPR011990. TPR-like_helical.
[Graphical view]
PANTHERPTHR10177:SF183. PTHR10177:SF183. 1 hit.
PfamPF02984. Cyclin_C. 1 hit.
PF00134. Cyclin_N. 1 hit.
PF00646. F-box. 1 hit.
[Graphical view]
SMARTSM00385. CYCLIN. 2 hits.
SM00256. FBOX. 1 hit.
[Graphical view]
SUPFAMSSF47954. SSF47954. 2 hits.
SSF81383. SSF81383. 1 hit.
PROSITEPS00292. CYCLINS. 1 hit.
PS50181. FBOX. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20867028.

Entry information

Entry nameCCNF_BOVIN
AccessionPrimary (citable) accession number: A5PK16
Entry history
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: July 10, 2007
Last modified: June 11, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families