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Protein

Adenylosuccinate synthetase isozyme 1

Gene

ADSSL1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Component of the purine nucleotide cycle (PNC), which interconverts IMP and AMP to regulate the nucleotide levels in various tissues, and which contributes to glycolysis and ammoniagenesis. Catalyzes the first committed step in the biosynthesis of AMP from IMP (By similarity).By similarity

Catalytic activityi

GTP + IMP + L-aspartate = GDP + phosphate + N(6)-(1,2-dicarboxyethyl)-AMP.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Pathwayi: AMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes AMP from IMP.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Adenylosuccinate synthetase isozyme 2 (ADSS), Adenylosuccinate synthetase isozyme 1 (ADSSL1)
  2. Adenylosuccinate lyase (ADSL), Adenylosuccinate lyase (ADSL)
This subpathway is part of the pathway AMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes AMP from IMP, the pathway AMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei43 – 431Proton acceptorUniRule annotation
Metal bindingi43 – 431MagnesiumUniRule annotation
Binding sitei43 – 431SubstrateUniRule annotation
Metal bindingi70 – 701Magnesium; via carbonyl oxygenUniRule annotation
Active sitei71 – 711Proton donorUniRule annotation
Binding sitei163 – 1631IMPUniRule annotation
Binding sitei177 – 1771IMP; shared with dimeric partnerUniRule annotation
Binding sitei256 – 2561IMPUniRule annotation
Binding sitei271 – 2711IMPUniRule annotation
Binding sitei335 – 3351IMPUniRule annotation
Binding sitei337 – 3371GTPUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi42 – 487GTPUniRule annotation
Nucleotide bindingi70 – 723GTPUniRule annotation
Nucleotide bindingi363 – 3653GTPUniRule annotation
Nucleotide bindingi445 – 4484GTPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

GTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-BTA-73817. Purine ribonucleoside monophosphate biosynthesis.
UniPathwayiUPA00075; UER00335.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenylosuccinate synthetase isozyme 1UniRule annotation (EC:6.3.4.4UniRule annotation)
Short name:
AMPSase 1UniRule annotation
Short name:
AdSS 1UniRule annotation
Alternative name(s):
Adenylosuccinate synthetase, basic isozymeUniRule annotation
Adenylosuccinate synthetase, muscle isozymeUniRule annotation
Short name:
M-type adenylosuccinate synthetaseUniRule annotation
IMP--aspartate ligase 1UniRule annotation
Gene namesi
Name:ADSSL1UniRule annotation
Synonyms:ADSS1UniRule annotation
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 21

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 457457Adenylosuccinate synthetase isozyme 1PRO_0000321960Add
BLAST

Proteomic databases

PaxDbiA5PJR4.
PRIDEiA5PJR4.

Expressioni

Gene expression databases

BgeeiENSBTAG00000017616.

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000041803.

Structurei

3D structure databases

ProteinModelPortaliA5PJR4.
SMRiA5PJR4. Positions 29-457.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni43 – 464IMP bindingUniRule annotation
Regioni68 – 714IMP bindingUniRule annotation
Regioni331 – 3377Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the adenylosuccinate synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiKOG1355. Eukaryota.
COG0104. LUCA.
GeneTreeiENSGT00390000015553.
HOGENOMiHOG000260959.
HOVERGENiHBG053768.
InParanoidiA5PJR4.
KOiK01939.
OMAiILPLHRE.
OrthoDBiEOG091G1BIK.
TreeFamiTF300486.

Family and domain databases

HAMAPiMF_00011. Adenylosucc_synth. 1 hit.
MF_03126. Adenylosucc_synth_vert_basic. 1 hit.
InterProiIPR018220. Adenylosuccin_syn_GTP-bd.
IPR033128. Adenylosuccin_syn_Lys_AS.
IPR001114. Adenylosuccinate_synthetase.
IPR027509. AdSS_1_vert.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR11846. PTHR11846. 1 hit.
PfamiPF00709. Adenylsucc_synt. 1 hit.
[Graphical view]
SMARTiSM00788. Adenylsucc_synt. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00184. purA. 1 hit.
PROSITEiPS01266. ADENYLOSUCCIN_SYN_1. 1 hit.
PS00513. ADENYLOSUCCIN_SYN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A5PJR4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGTRASNDR PPSAGGVKRG RLQHEAATTG SRVTVVLGAQ WGDEGKGKVV
60 70 80 90 100
DLLATDADVV SRCQGGNNAG HTVVVDGKEY DFHLLPSGII NPKAVSFIGN
110 120 130 140 150
GVVVHLPGLF EEAEKNEKKG LKDWEKRLVI SDRAHLVFDF HQAVDGLQEV
160 170 180 190 200
QRQAQEGKNI GTTRKGIGPA YSSKAARTGL RICDLLSDFD EFSSRFKNLA
210 220 230 240 250
RQHQSMFPSL EVDVEGQLKR LKGFAERIRP MVRDGVYFMY EALHGPPKKI
260 270 280 290 300
LVEGANAALL DIDFGTYPFV TSSNCTVGGV CTGLGIPPQN IGEVYGVVKA
310 320 330 340 350
YTTRVGIGAF PTEQINEIGD LLQSRGHEWG VTTGRKRRCG WLDLMILRYA
360 370 380 390 400
HMVNGFTALA LTKLDILDAL DEIKVGVAYK LGGKRIPYFP ANQEILQKVE
410 420 430 440 450
VEYETLPGWK ADTTGARKWE DLPPQAQSYI RFVENHVGVA VKWVGVGKSR

DSMIQLF
Length:457
Mass (Da):50,134
Last modified:July 10, 2007 - v1
Checksum:i202C6350F68068F5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC142211 mRNA. Translation: AAI42212.1.
RefSeqiNP_001092662.1. NM_001099192.1.
UniGeneiBt.16782.

Genome annotation databases

EnsembliENSBTAT00000044296; ENSBTAP00000041803; ENSBTAG00000017616.
GeneIDi784089.
KEGGibta:784089.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC142211 mRNA. Translation: AAI42212.1.
RefSeqiNP_001092662.1. NM_001099192.1.
UniGeneiBt.16782.

3D structure databases

ProteinModelPortaliA5PJR4.
SMRiA5PJR4. Positions 29-457.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000041803.

Proteomic databases

PaxDbiA5PJR4.
PRIDEiA5PJR4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000044296; ENSBTAP00000041803; ENSBTAG00000017616.
GeneIDi784089.
KEGGibta:784089.

Organism-specific databases

CTDi122622.

Phylogenomic databases

eggNOGiKOG1355. Eukaryota.
COG0104. LUCA.
GeneTreeiENSGT00390000015553.
HOGENOMiHOG000260959.
HOVERGENiHBG053768.
InParanoidiA5PJR4.
KOiK01939.
OMAiILPLHRE.
OrthoDBiEOG091G1BIK.
TreeFamiTF300486.

Enzyme and pathway databases

UniPathwayiUPA00075; UER00335.
ReactomeiR-BTA-73817. Purine ribonucleoside monophosphate biosynthesis.

Gene expression databases

BgeeiENSBTAG00000017616.

Family and domain databases

HAMAPiMF_00011. Adenylosucc_synth. 1 hit.
MF_03126. Adenylosucc_synth_vert_basic. 1 hit.
InterProiIPR018220. Adenylosuccin_syn_GTP-bd.
IPR033128. Adenylosuccin_syn_Lys_AS.
IPR001114. Adenylosuccinate_synthetase.
IPR027509. AdSS_1_vert.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR11846. PTHR11846. 1 hit.
PfamiPF00709. Adenylsucc_synt. 1 hit.
[Graphical view]
SMARTiSM00788. Adenylsucc_synt. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00184. purA. 1 hit.
PROSITEiPS01266. ADENYLOSUCCIN_SYN_1. 1 hit.
PS00513. ADENYLOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPURA1_BOVIN
AccessioniPrimary (citable) accession number: A5PJR4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: July 10, 2007
Last modified: September 7, 2016
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.