A5PJR4 (PURA1_BOVIN) Reviewed, UniProtKB/Swiss-Prot
Last modified
November 16, 2011.
Version 38.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Adenylosuccinate synthetase isozyme 1 Short name=AMPSase 1 Short name=AdSS 1 EC=6.3.4.4 Alternative name(s): Adenylosuccinate synthetase, basic isozyme Adenylosuccinate synthetase, muscle isozyme Short name=M-type adenylosuccinate synthetase IMP--aspartate ligase 1 | ||||
| Gene names |
| ||||
| Organism | Bos taurus (Bovine) | ||||
| Taxonomic identifier | 9913 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Protein attributes
| Sequence length | 457 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at transcript level |
General annotation (Comments)
| Function | Component of the purine nucleotide cycle (PNC), which interconverts IMP and AMP to regulate the nucleotide levels in various tissues, and which contributes to glycolysis and ammoniagenesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP By similarity. |
| Catalytic activity | GTP + IMP + L-aspartate = GDP + phosphate + N(6)-(1,2-dicarboxyethyl)-AMP. |
| Cofactor | Binds 1 magnesium ion per subunit By similarity. |
| Pathway | Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2. |
| Subunit structure | Homodimer By similarity. |
| Subcellular location | Cytoplasm By similarity. |
| Sequence similarities | Belongs to the adenylosuccinate synthetase family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Purine biosynthesis |
| Cellular component | Cytoplasm |
| Ligand | GTP-binding Magnesium Metal-binding Nucleotide-binding |
| Molecular function | Ligase |
| PTM | Phosphoprotein |
| Technical term | Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological process | purine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | GTP binding Inferred from electronic annotation. Source: UniProtKB-KW adenylosuccinate synthase activityInferred from electronic annotation. Source: EC magnesium ion bindingInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 457 | 457 | Adenylosuccinate synthetase isozyme 1 | PRO_0000321960 | |||||
Regions | |||||||||
| Nucleotide binding | 42 – 48 | 7 | GTP By similarity | ||||||
| Nucleotide binding | 70 – 72 | 3 | GTP By similarity | ||||||
| Nucleotide binding | 363 – 365 | 3 | GTP By similarity | ||||||
| Nucleotide binding | 445 – 448 | 4 | GTP By similarity | ||||||
| Region | 43 – 46 | 4 | IMP binding By similarity | ||||||
| Region | 68 – 71 | 4 | IMP binding By similarity | ||||||
| Region | 331 – 337 | 7 | Substrate binding By similarity | ||||||
Sites | |||||||||
| Active site | 43 | 1 | Proton acceptor By similarity | ||||||
| Active site | 71 | 1 | Proton donor By similarity | ||||||
| Metal binding | 43 | 1 | Magnesium By similarity | ||||||
| Metal binding | 70 | 1 | Magnesium; via carbonyl oxygen By similarity | ||||||
| Binding site | 43 | 1 | Substrate By similarity | ||||||
| Binding site | 163 | 1 | IMP By similarity | ||||||
| Binding site | 177 | 1 | IMP; shared with dimeric partner By similarity | ||||||
| Binding site | 256 | 1 | IMP By similarity | ||||||
| Binding site | 271 | 1 | IMP By similarity | ||||||
| Binding site | 335 | 1 | IMP By similarity | ||||||
| Binding site | 337 | 1 | GTP By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 388 | 1 | Phosphotyrosine By similarity | ||||||
Sequences
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References
| [1] | NIH - Mammalian Gene Collection (MGC) project Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Fetal muscle. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | BC142211 mRNA. Translation: AAI42212.1. |
| IPI | IPI00707722. |
| RefSeq | NP_001092662.1. NM_001099192.1. |
| UniGene | Bt.16782. |
3D structure databases | |
| ProteinModelPortal | A5PJR4. |
| SMR | A5PJR4. Positions 29-457. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | A5PJR4. |
Proteomic databases | |
| PRIDE | A5PJR4. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| Ensembl | ENSBTAT00000044296; ENSBTAP00000041803; ENSBTAG00000017616. |
| GeneID | 784089. |
| KEGG | bta:784089. |
Organism-specific databases | |
| CTD | 122622. |
Phylogenomic databases | |
| eggNOG | maNOG07116. |
| GeneTree | ENSGT00390000015553. |
| HOVERGEN | HBG053768. |
| InParanoid | A5PJR4. |
| OrthoDB | EOG4P5K90. |
Family and domain databases | |
| InterPro | IPR018220. Adenylosuccinate_synthase_AS. IPR001114. Adenylosuccinate_synthetase. [Graphical view] |
| KO | K01939. |
| PANTHER | PTHR11846. Asucc_synthtase. 1 hit. |
| Pfam | PF00709. Adenylsucc_synt. 1 hit. [Graphical view] |
| SMART | SM00788. Adenylsucc_synt. 1 hit. [Graphical view] |
| TIGRFAMs | TIGR00184. PurA. 1 hit. |
| PROSITE | PS01266. ADENYLOSUCCIN_SYN_1. 1 hit. PS00513. ADENYLOSUCCIN_SYN_2. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | PURA1_BOVIN | ||||||||
| Accession | Primary (citable) accession number: A5PJR4 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Chordata Protein Annotation Program | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

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