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A5PF10 (NEUR1_PIG) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Sialidase-1

EC=3.2.1.18
Alternative name(s):
Acetylneuraminyl hydrolase
Lysosomal sialidase
N-acetyl-alpha-neuraminidase 1
Gene names
Name:NEU1
OrganismSus scrofa (Pig) [Reference proteome]
Taxonomic identifier9823 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus

Protein attributes

Sequence length416 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the removal of sialic acid (N-acetylneuraminic acid) moities from glycoproteins and glycolipids. To be active, it is strictly dependent on its presence in the multienzyme complex. Appears to have a preference for alpha 2-3 and alpha 2-6 sialyl linkage By similarity.

Catalytic activity

Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.

Subunit structure

Interacts with cathepsin A (protective protein), beta-galactosidase and N-acetylgalactosamine-6-sulfate sulfatase in a multienzyme complex By similarity.

Subcellular location

Lysosome membrane; Peripheral membrane protein; Lumenal side By similarity. Lysosome lumen By similarity. Cell membrane By similarity. Cytoplasmic vesicle By similarity. Note: Localized not only on the inner side of the lysosomal membrane and in the lysosomal lumen, but also on the plasma membrane and in intracellular vesicles By similarity.

Domain

A C-terminal internalization signal (YGTL) appears to allow the targeting of plasma membrane proteins to endosomes By similarity.

Post-translational modification

N-glycosylated By similarity.

Phosphorylation of tyrosine within the internalization signal results in inhibition of sialidase internalization and blockage on the plasma membrane By similarity.

Sequence similarities

Belongs to the glycosyl hydrolase 33 family.

Contains 4 BNR repeats.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 4848 By similarity
Chain49 – 416368Sialidase-1
PRO_0000304727

Regions

Repeat113 – 12412BNR 1
Repeat173 – 18412BNR 2
Repeat232 – 24312BNR 3
Repeat348 – 35912BNR 4
Motif78 – 814FRIP motif
Motif413 – 4164Internalization signal

Sites

Active site1041Proton acceptor By similarity
Active site3711Nucleophile By similarity
Active site3951 Potential
Binding site791Substrate By similarity
Binding site981Substrate By similarity
Binding site2651Substrate By similarity
Binding site2811Substrate By similarity
Binding site3421Substrate By similarity

Amino acid modifications

Glycosylation1871N-linked (GlcNAc...) Potential
Glycosylation3441N-linked (GlcNAc...) Potential
Glycosylation3531N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
A5PF10 [UniParc].

Last modified July 10, 2007. Version 1.
Checksum: 65E99172C69F3EB8

FASTA41645,159
        10         20         30         40         50         60 
MTAERPGAVP LGRPGRPPML GLGEAYRAQV FASIFLLLLS PAGVGARAKN DFNLVHPLVT 

        70         80         90        100        110        120 
MEQLLWVSGK QIGSVDTFRI PLITTTPRGT LLAFAEARKM SASDKGAKFI ALRRSMDQGS 

       130        140        150        160        170        180 
TWSPTAFIVD DGETPDGLNL GAVVSDTTTG VVFLFYSLCA HKAGCRVAST MLVWSKDDGI 

       190        200        210        220        230        240 
SWSSPRNLSL DIGTEMFAPG PGSGIQKQWA PQKGRLIVCG HGTLERDGVF CLLSDDHGAS 

       250        260        270        280        290        300 
WRYGSGISGI PYGQPKREND FNPDECQPYE LPDGSVVINA RNQNNYHCRC RIVLRSYDAC 

       310        320        330        340        350        360 
DTLRPRDVTF DPELVDPVVA AGAVATSSGI IFFSNPAHPE FRVNLTLRWS FSNGTSWRKE 

       370        380        390        400        410 
TVQIWPGPSG YSSLATLEGS VGGEDQAPQL YVLYEKGRNR YTESISLAKV SVYGTL 

« Hide

References

[1]Porcine genome sequencing project
Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL773527 Genomic DNA. Translation: CAN87707.1.
RefSeqNP_001095292.1. NM_001101822.1.
UniGeneSsc.51944.

3D structure databases

ProteinModelPortalA5PF10.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9823.ENSSSCP00000001513.

Protein family/group databases

CAZyGH33. Glycoside Hydrolase Family 33.

Proteomic databases

PaxDbA5PF10.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID100124381.
KEGGssc:100124381.

Organism-specific databases

CTD4758.

Phylogenomic databases

eggNOGCOG4409.
HOGENOMHOG000007651.
HOVERGENHBG057314.
KOK01186.

Family and domain databases

Gene3D2.120.10.10. 1 hit.
InterProIPR026856. Sialidase_fam.
IPR011040. Sialidases.
[Graphical view]
PANTHERPTHR10628. PTHR10628. 1 hit.
SUPFAMSSF50939. SSF50939. 1 hit.
ProtoNetSearch...

Entry information

Entry nameNEUR1_PIG
AccessionPrimary (citable) accession number: A5PF10
Entry history
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: July 10, 2007
Last modified: June 11, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries