Reviewed, UniProtKB/Swiss-Prot A5PF10 (NEUR1_PIG)

Names and origin

Protein names

Recommended name:
    Sialidase-1
    EC=3.2.1.18
Alternative name(s):
    Lysosomal sialidase
    N-acetyl-alpha-neuraminidase 1
    Acetylneuraminyl hydrolase

Gene names

Name:

NEU1

Organism

Sus scrofa (Pig)

Taxonomic identifier

9823 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus

Protein attributes

Sequence length	416 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the removal of sialic acid (N-acetylneuramic acid) moities from glycoproteins and glycolipids. To be active, it is strictly dependent on its presence in the multienzyme complex. Appears to have a preference for alpha 2-3 and alpha 2-6 sialyl linkage By similarity.
Catalytic activity	Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.
Subunit structure	Interacts with cathepsin A (protective protein), beta-galactosidase and N-acetylgalactosamine-6-sulfate sulfatase in a multienzyme complex By similarity.
Subcellular location	Lysosome membrane; Peripheral membrane protein; Lumenal side. Lysosome lumen. Cell membrane. Cytoplasmic vesicle. Note: Localized not only on the inner side of the lysosomal membrane and in the lysosomal lumen, but also on the plasma membrane and in intracellular vesicles By similarity.
Domain	A C-terminal internalization signal (YGTL) appears to allow the targeting of plasma membrane proteins to endosomes By similarity.
Post-translational modification	N-glycosylated By similarity. Phosphorylation of tyrosine within the internalization signal results in inhibition of sialidase internalization and blockage on the plasma membrane By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 33 family. Contains 4 BNR repeats.

Ontologies

Keywords
Cellular component	Cell membrane Cytoplasmic vesicle Lysosome Membrane
Domain	Repeat Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein Phosphoprotein
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasmic membrane-bounded vesicle Inferred from electronic annotation. Source: UniProtKB-SubCell extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell lysosomal lumen Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	exo-alpha-sialidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 48	48	By similarity
	Chain	49 – 416	368	Sialidase-1		PRO_0000304727
Regions
	Repeat	113 – 124	12	BNR 1
	Repeat	173 – 184	12	BNR 2
	Repeat	232 – 243	12	BNR 3
	Repeat	348 – 359	12	BNR 4
	Motif	78 – 81	4	FRIP motif
	Motif	413 – 416	4	Internalization signal
Sites
	Active site	104	1	Proton acceptor By similarity
	Active site	371	1	Nucleophile By similarity
	Active site	395	1	Potential
	Binding site	79	1	Substrate By similarity
	Binding site	281	1	Substrate By similarity
	Binding site	342	1	Substrate By similarity
Amino acid modifications
	Modified residue	243	1	Phosphotyrosine By similarity
	Modified residue	245	1	Phosphoserine By similarity
	Modified residue	252	1	Phosphotyrosine By similarity
	Glycosylation	187	1	N-linked (GlcNAc...) Potential
	Glycosylation	344	1	N-linked (GlcNAc...) Potential
	Glycosylation	353	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	A5PF10-1 [UniParc]. Last modified July 10, 2007. Version 1. Checksum: 65E99172C69F3EB8	FASTA	416	45,159
	10 20 30 40 50 60 MTAERPGAVP LGRPGRPPML GLGEAYRAQV FASIFLLLLS PAGVGARAKN DFNLVHPLVT 70 80 90 100 110 120 MEQLLWVSGK QIGSVDTFRI PLITTTPRGT LLAFAEARKM SASDKGAKFI ALRRSMDQGS 130 140 150 160 170 180 TWSPTAFIVD DGETPDGLNL GAVVSDTTTG VVFLFYSLCA HKAGCRVAST MLVWSKDDGI 190 200 210 220 230 240 SWSSPRNLSL DIGTEMFAPG PGSGIQKQWA PQKGRLIVCG HGTLERDGVF CLLSDDHGAS 250 260 270 280 290 300 WRYGSGISGI PYGQPKREND FNPDECQPYE LPDGSVVINA RNQNNYHCRC RIVLRSYDAC 310 320 330 340 350 360 DTLRPRDVTF DPELVDPVVA AGAVATSSGI IFFSNPAHPE FRVNLTLRWS FSNGTSWRKE 370 380 390 400 410 TVQIWPGPSG YSSLATLEGS VGGEDQAPQL YVLYEKGRNR YTESISLAKV SVYGTL

References

[1]	Porcine genome sequencing project Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Duroc.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL773527 Genomic DNA. Translation: CAN87707.1.
RefSeq	NP_001095292.1.
UniGene	Ssc.51944
3D structure databases
SMR	A5PF10. Positions 35-410, 75-412.
ModBase	Search...
Genome annotation databases
Ensembl	ENSSSCT00000001555; ENSSSCP00000001513; ENSSSCG00000001418; Sus scrofa. [Genome view]
GeneID	100124381.
KEGG	ssc:100124381.
Organism-specific databases
CTD	100124381.
Phylogenomic databases
OrthoDB	EOG96X1TS.
Enzyme and pathway databases
BRENDA	3.2.1.18. 249.
Family and domain databases
InterPro	IPR011040. Neuraminidase. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NEUR1_PIG

Accession

Primary (citable) accession number: A5PF10

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	July 10, 2007
Last modified:	February 9, 2010
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)