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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Clostridium kluyveri (strain ATCC 8527 / DSM 555 / NCIMB 10680)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei254ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:CKL_1456
OrganismiClostridium kluyveri (strain ATCC 8527 / DSM 555 / NCIMB 10680)
Taxonomic identifieri431943 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
Proteomesi
  • UP000002411 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003309641 – 487Glutamate--tRNA ligaseAdd BLAST487

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi431943.CKL_1456

Structurei

3D structure databases

ProteinModelPortaliA5N867
SMRiA5N867
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi10 – 20"HIGH" regionUniRule annotationAdd BLAST11
Motifi251 – 255"KMSKS" regionUniRule annotation5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C20 Bacteria
COG0008 LUCA
HOGENOMiHOG000252720
KOiK01885
OMAiHYINTLP
OrthoDBiPOG091H021W

Family and domain databases

CDDicd00808 GluRS_core, 1 hit
Gene3Di1.10.10.350, 1 hit
3.40.50.620, 1 hit
HAMAPiMF_00022 Glu_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR008925 aa-tRNA-synth_I_codon-bd
IPR020751 aa-tRNA-synth_I_codon-bd_sub2
IPR001412 aa-tRNA-synth_I_CS
IPR004527 Glu-tRNA-ligase_bac/mito
IPR000924 Glu/Gln-tRNA-synth
IPR020058 Glu/Gln-tRNA-synth_Ib_cat-dom
IPR033910 GluRS_core
IPR014729 Rossmann-like_a/b/a_fold
PfamiView protein in Pfam
PF00749 tRNA-synt_1c, 1 hit
PRINTSiPR00987 TRNASYNTHGLU
SUPFAMiSSF48163 SSF48163, 1 hit
TIGRFAMsiTIGR00464 gltX_bact, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

A5N867-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKVRTRFAP SPTGYMHVGN LRTALYTYLI AKHDGGDFIL RIEDTDQERF
60 70 80 90 100
VEGALDIIYH TLEITGLKHD EGPDIGGPVG PYIQSQRTDI YLEYAKELID
110 120 130 140 150
KGKAYYCFCT KERMDSLKNK DDEEKEFYKY DKHCLKLSKE EINEKLASNI
160 170 180 190 200
PYVIRQNNPE SGFTTFHDEI YGDISVDNSE LDDMILIKSD GYPTYNFANV
210 220 230 240 250
VDDHLMGITH VVRGSEYLSS APKYNRLYDA FGWEVPIYIH CPPIMKDAHQ
260 270 280 290 300
KLSKRNGDAS FQDLIEKGYL KEAVLNYIAL LGWNPGNEKE IFDLDELVEL
310 320 330 340 350
FNYKNINKSP AIFDNVKLKW MNGEYMKKLP LEEFNKMALP YYKKVISKNL
360 370 380 390 400
DFLKISELLK IRVEILSEIP DMLDFFNELP EYSTEIYIHK KMKTNLENSL
410 420 430 440 450
FTLEKILPKF KELSPWTLEN IEKCCMDLIS ELQVKNGIVL WPVRIALSGK
460 470 480
KSTPGGAFEI ADIIGKDESL KRIEYGIKKL KLESGDN
Length:487
Mass (Da):56,293
Last modified:July 10, 2007 - v1
Checksum:i8846D2DA9274D6C8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000673 Genomic DNA Translation: EDK33498.1
RefSeqiWP_012101848.1, NC_009706.1

Genome annotation databases

EnsemblBacteriaiEDK33498; EDK33498; CKL_1456
KEGGickl:CKL_1456

Similar proteinsi

Entry informationi

Entry nameiSYE_CLOK5
AccessioniPrimary (citable) accession number: A5N867
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: July 10, 2007
Last modified: March 28, 2018
This is version 73 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families
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