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Protein

16S rRNA (cytosine(967)-C(5))-methyltransferase

Gene

CKL_1372

Organism
Clostridium kluyveri (strain ATCC 8527 / DSM 555 / NCIMB 10680)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA.SAAS annotation

Catalytic activityi

S-adenosyl-L-methionine + cytosine(967) in 16S rRNA = S-adenosyl-L-homocysteine + 5-methylcytosine(967) in 16S rRNA.SAAS annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei283S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei310S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei328S-adenosyl-L-methioninePROSITE-ProRule annotation1
Active sitei381NucleophilePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferasePROSITE-ProRule annotationSAAS annotation, RNA-bindingPROSITE-ProRule annotationSAAS annotation, Transferase
Biological processrRNA processingSAAS annotation
LigandS-adenosyl-L-methioninePROSITE-ProRule annotationSAAS annotation

Names & Taxonomyi

Protein namesi
Recommended name:
16S rRNA (cytosine(967)-C(5))-methyltransferaseSAAS annotation (EC:2.1.1.176SAAS annotation)
Gene namesi
Ordered Locus Names:CKL_1372Imported
OrganismiClostridium kluyveri (strain ATCC 8527 / DSM 555 / NCIMB 10680)Imported
Taxonomic identifieri431943 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
Proteomesi
  • UP000002411 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm SAAS annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmSAAS annotation

Interactioni

Protein-protein interaction databases

STRINGi431943.CKL_1372.

Structurei

3D structure databases

ProteinModelPortaliA5N7Y3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini169 – 442SAM_MT_RSMB_NOPInterPro annotationAdd BLAST274

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.PROSITE-ProRule annotationSAAS annotation

Phylogenomic databases

eggNOGiENOG4105CYJ. Bacteria.
COG0144. LUCA.
HOGENOMiHOG000037300.
KOiK03500.
OMAiHIGLYQL.
OrthoDBiPOG091H02E2.

Family and domain databases

Gene3Di1.10.940.10. 1 hit.
3.40.50.150. 1 hit.
InterProiView protein in InterPro
IPR001678. MeTrfase_RsmB/NOP2.
IPR006027. NusB_RsmB_TIM44.
IPR023267. RCMT.
IPR004573. rRNA_ssu_MeTfrase_B.
IPR029063. SAM-dependent_MTases.
PfamiView protein in Pfam
PF01189. Methyltr_RsmB-F. 1 hit.
PF01029. NusB. 1 hit.
PRINTSiPR02008. RCMTFAMILY.
SUPFAMiSSF48013. SSF48013. 1 hit.
SSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00563. rsmB. 1 hit.
PROSITEiView protein in PROSITE
PS51686. SAM_MT_RSMB_NOP. 1 hit.

Sequencei

Sequence statusi: Complete.

A5N7Y3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNSARSVAVE VVKKVLYDNA YSSIVLNKYL NKFDLNKKDR ALITELVYGT
60 70 80 90 100
LKYKYTIDCI LKNFIKSNLE KMNKDILNML RISICQLRYL DKIPEFAVVN
110 120 130 140 150
EAVEFSKNIS FKLSKLVNGV LRNYLRNKSN SFGGNKEDKI EGICFEYSFP
160 170 180 190 200
RWMAELFINQ YGEDLGKFIM GSLNVVPKVT VRINTLKTDY KHAWNELIVN
210 220 230 240 250
GYDVKRGNVY KEAVIINKGS NIELNPLFEK GHITVQDESA MLVSYFMDVE
260 270 280 290 300
ENMTILDLCS APGGKSCHIG ELMKNTGNVH CYDLYESKLS LVRHNAERLG
310 320 330 340 350
IKNITCKKLD AAKYLEKLKD TADRVLIDVP CSGLGIIRKK PEIKWNKKED
360 370 380 390 400
SLKDLIEIQR KIILNAARYV KVGGKLVYST CTLNKKENEE NINWFISRNP
410 420 430 440
QYKLDKLYCG DFHNIIYHEE GYMTILPDNH MDGFFIARLI KQR
Length:443
Mass (Da):51,137
Last modified:July 10, 2007 - v1
Checksum:iE2061A6B9281814F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000673 Genomic DNA. Translation: EDK33414.1.
RefSeqiWP_012101761.1. NC_009706.1.

Genome annotation databases

EnsemblBacteriaiEDK33414; EDK33414; CKL_1372.
KEGGickl:CKL_1372.
PATRICi19464201. VBICloKlu111549_1435.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000673 Genomic DNA. Translation: EDK33414.1.
RefSeqiWP_012101761.1. NC_009706.1.

3D structure databases

ProteinModelPortaliA5N7Y3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi431943.CKL_1372.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiEDK33414; EDK33414; CKL_1372.
KEGGickl:CKL_1372.
PATRICi19464201. VBICloKlu111549_1435.

Phylogenomic databases

eggNOGiENOG4105CYJ. Bacteria.
COG0144. LUCA.
HOGENOMiHOG000037300.
KOiK03500.
OMAiHIGLYQL.
OrthoDBiPOG091H02E2.

Family and domain databases

Gene3Di1.10.940.10. 1 hit.
3.40.50.150. 1 hit.
InterProiView protein in InterPro
IPR001678. MeTrfase_RsmB/NOP2.
IPR006027. NusB_RsmB_TIM44.
IPR023267. RCMT.
IPR004573. rRNA_ssu_MeTfrase_B.
IPR029063. SAM-dependent_MTases.
PfamiView protein in Pfam
PF01189. Methyltr_RsmB-F. 1 hit.
PF01029. NusB. 1 hit.
PRINTSiPR02008. RCMTFAMILY.
SUPFAMiSSF48013. SSF48013. 1 hit.
SSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00563. rsmB. 1 hit.
PROSITEiView protein in PROSITE
PS51686. SAM_MT_RSMB_NOP. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiA5N7Y3_CLOK5
AccessioniPrimary (citable) accession number: A5N7Y3
Entry historyiIntegrated into UniProtKB/TrEMBL: July 10, 2007
Last sequence update: July 10, 2007
Last modified: February 15, 2017
This is version 77 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.