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A5N7P5

- GLYA_CLOK5

UniProt

A5N7P5 - GLYA_CLOK5

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Protein

Serine hydroxymethyltransferase

Gene

glyA

Organism
Clostridium kluyveri (strain ATCC 8527 / DSM 555 / NCIMB 10680)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine.UniRule annotation

Cofactori

Pyridoxal phosphate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei33 – 331Pyridoxal phosphateUniRule annotation
Binding sitei53 – 531Pyridoxal phosphateUniRule annotation
Binding sitei55 – 551SubstrateUniRule annotation
Binding sitei62 – 621SubstrateUniRule annotation
Binding sitei63 – 631Pyridoxal phosphateUniRule annotation
Binding sitei97 – 971Pyridoxal phosphateUniRule annotation
Binding sitei119 – 1191Substrate; via carbonyl oxygenUniRule annotation
Binding sitei174 – 1741Pyridoxal phosphateUniRule annotation
Binding sitei202 – 2021Pyridoxal phosphateUniRule annotation
Binding sitei227 – 2271Pyridoxal phosphateUniRule annotation
Binding sitei234 – 2341Pyridoxal phosphateUniRule annotation
Binding sitei259 – 2591Pyridoxal phosphate; via amide nitrogen and carbonyl oxygenUniRule annotation
Binding sitei359 – 3591Pyridoxal phosphateUniRule annotation

GO - Molecular functioni

  1. glycine hydroxymethyltransferase activity Source: UniProtKB-HAMAP
  2. pyridoxal phosphate binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. glycine biosynthetic process from serine Source: UniProtKB-HAMAP
  2. tetrahydrofolate interconversion Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Amino-acid biosynthesis, One-carbon metabolism

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciCKLU431943:GJF1-1284-MONOMER.
UniPathwayiUPA00193.
UPA00288; UER01023.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine hydroxymethyltransferaseUniRule annotation (EC:2.1.2.1UniRule annotation)
Short name:
SHMTUniRule annotation
Short name:
Serine methylaseUniRule annotation
Gene namesi
Name:glyAUniRule annotation
Ordered Locus Names:CKL_1284
OrganismiClostridium kluyveri (strain ATCC 8527 / DSM 555 / NCIMB 10680)
Taxonomic identifieri431943 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
ProteomesiUP000002411: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 411411Serine hydroxymethyltransferasePRO_1000074891Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei228 – 2281N6-(pyridoxal phosphate)lysineUniRule annotation

Proteomic databases

PRIDEiA5N7P5.

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi431943.CKL_1284.

Structurei

3D structure databases

ProteinModelPortaliA5N7P5.
SMRiA5N7P5. Positions 6-388.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni123 – 1253Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the SHMT family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0112.
HOGENOMiHOG000239404.
KOiK00600.
OMAiHLMLVDV.
OrthoDBiEOG6Z0QB2.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
HAMAPiMF_00051. SHMT.
InterProiIPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
IPR001085. Ser_HO-MeTrfase.
IPR019798. Ser_HO-MeTrfase_PLP_BS.
[Graphical view]
PANTHERiPTHR11680. PTHR11680. 1 hit.
PfamiPF00464. SHMT. 1 hit.
[Graphical view]
PIRSFiPIRSF000412. SHMT. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00096. SHMT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A5N7P5 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDFNELKNTD KDIYGIIEEE WERQKNGIEL IASENFTSKS VMEAMGSFLT
60 70 80 90 100
NKYAEGYPGK RYYGGCYIVD KAEDLARDRM KKLFNAEHVN VQPHSGSQAN
110 120 130 140 150
MAVYMSVLKP GDTVLGMSLN HGGHLTHGSK VSFSGKLYNF VSYGLNSDTE
160 170 180 190 200
IIDYDEMREL ALKHKPKMIV SGASAYPRKI DFKKIREICD EVGAYMMVDM
210 220 230 240 250
AHIAGIIAAG RHESPVPYAD FVTTTTHKTL RGPRGGAIIC KEKYGAALDK
260 270 280 290 300
TIFPGIQGGP LMHIIAAKAV CFGEALKDEY KEYIDQIIKN AKVFGEELVK
310 320 330 340 350
YGFRLVSGGT DNHLLLVDLT NKNITGKDLE ELLDKVNITV NKNAIPFDKL
360 370 380 390 400
KPNVTSGIRV GTPAVTTRGF KEEEMKKVAY FINKAVENRE GDLSAIKREV
410
IELCEAFPLY E
Length:411
Mass (Da):45,809
Last modified:July 10, 2007 - v1
Checksum:i626466A21D11E446
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000673 Genomic DNA. Translation: EDK33326.1.
RefSeqiYP_001394674.1. NC_009706.1.

Genome annotation databases

EnsemblBacteriaiEDK33326; EDK33326; CKL_1284.
GeneIDi5392545.
KEGGickl:CKL_1284.
PATRICi19464019. VBICloKlu111549_1344.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000673 Genomic DNA. Translation: EDK33326.1 .
RefSeqi YP_001394674.1. NC_009706.1.

3D structure databases

ProteinModelPortali A5N7P5.
SMRi A5N7P5. Positions 6-388.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 431943.CKL_1284.

Proteomic databases

PRIDEi A5N7P5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai EDK33326 ; EDK33326 ; CKL_1284 .
GeneIDi 5392545.
KEGGi ckl:CKL_1284.
PATRICi 19464019. VBICloKlu111549_1344.

Phylogenomic databases

eggNOGi COG0112.
HOGENOMi HOG000239404.
KOi K00600.
OMAi HLMLVDV.
OrthoDBi EOG6Z0QB2.

Enzyme and pathway databases

UniPathwayi UPA00193 .
UPA00288 ; UER01023 .
BioCyci CKLU431943:GJF1-1284-MONOMER.

Family and domain databases

Gene3Di 3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
HAMAPi MF_00051. SHMT.
InterProi IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
IPR001085. Ser_HO-MeTrfase.
IPR019798. Ser_HO-MeTrfase_PLP_BS.
[Graphical view ]
PANTHERi PTHR11680. PTHR11680. 1 hit.
Pfami PF00464. SHMT. 1 hit.
[Graphical view ]
PIRSFi PIRSF000412. SHMT. 1 hit.
SUPFAMi SSF53383. SSF53383. 1 hit.
PROSITEi PS00096. SHMT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 8527 / DSM 555 / NCIMB 10680.

Entry informationi

Entry nameiGLYA_CLOK5
AccessioniPrimary (citable) accession number: A5N7P5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: July 10, 2007
Last modified: October 29, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3