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A5N7P5

- GLYA_CLOK5

UniProt

A5N7P5 - GLYA_CLOK5

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Protein
Serine hydroxymethyltransferase
Gene
glyA, CKL_1284
Organism
Clostridium kluyveri (strain ATCC 8527 / DSM 555 / NCIMB 10680)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism By similarity.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine.UniRule annotation

Cofactori

Pyridoxal phosphate By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei33 – 331Pyridoxal phosphate By similarity
Binding sitei53 – 531Pyridoxal phosphate By similarity
Binding sitei55 – 551Substrate By similarity
Binding sitei62 – 621Substrate By similarity
Binding sitei63 – 631Pyridoxal phosphate By similarity
Binding sitei97 – 971Pyridoxal phosphate By similarity
Binding sitei119 – 1191Substrate; via carbonyl oxygen By similarity
Binding sitei174 – 1741Pyridoxal phosphate By similarity
Binding sitei202 – 2021Pyridoxal phosphate By similarity
Binding sitei227 – 2271Pyridoxal phosphate By similarity
Binding sitei234 – 2341Pyridoxal phosphate By similarity
Binding sitei259 – 2591Pyridoxal phosphate; via amide nitrogen and carbonyl oxygen By similarity
Binding sitei359 – 3591Pyridoxal phosphate By similarity

GO - Molecular functioni

  1. glycine hydroxymethyltransferase activity Source: UniProtKB-HAMAP
  2. pyridoxal phosphate binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. glycine biosynthetic process from serine Source: UniProtKB-HAMAP
  2. tetrahydrofolate interconversion Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Amino-acid biosynthesis, One-carbon metabolism

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciCKLU431943:GJF1-1284-MONOMER.
UniPathwayiUPA00193.
UPA00288; UER01023.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine hydroxymethyltransferase (EC:2.1.2.1)
Short name:
SHMT
Short name:
Serine methylase
Gene namesi
Name:glyA
Ordered Locus Names:CKL_1284
OrganismiClostridium kluyveri (strain ATCC 8527 / DSM 555 / NCIMB 10680)
Taxonomic identifieri431943 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
ProteomesiUP000002411: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 411411Serine hydroxymethyltransferaseUniRule annotation
PRO_1000074891Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei228 – 2281N6-(pyridoxal phosphate)lysine By similarity

Proteomic databases

PRIDEiA5N7P5.

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi431943.CKL_1284.

Structurei

3D structure databases

ProteinModelPortaliA5N7P5.
SMRiA5N7P5. Positions 6-388.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni123 – 1253Substrate binding By similarity

Sequence similaritiesi

Belongs to the SHMT family.

Phylogenomic databases

eggNOGiCOG0112.
HOGENOMiHOG000239404.
KOiK00600.
OMAiHLMLVDV.
OrthoDBiEOG6Z0QB2.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
HAMAPiMF_00051. SHMT.
InterProiIPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
IPR001085. Ser_HO-MeTrfase.
IPR019798. Ser_HO-MeTrfase_PLP_BS.
[Graphical view]
PANTHERiPTHR11680. PTHR11680. 1 hit.
PfamiPF00464. SHMT. 1 hit.
[Graphical view]
PIRSFiPIRSF000412. SHMT. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00096. SHMT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A5N7P5-1 [UniParc]FASTAAdd to Basket

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MDFNELKNTD KDIYGIIEEE WERQKNGIEL IASENFTSKS VMEAMGSFLT    50
NKYAEGYPGK RYYGGCYIVD KAEDLARDRM KKLFNAEHVN VQPHSGSQAN 100
MAVYMSVLKP GDTVLGMSLN HGGHLTHGSK VSFSGKLYNF VSYGLNSDTE 150
IIDYDEMREL ALKHKPKMIV SGASAYPRKI DFKKIREICD EVGAYMMVDM 200
AHIAGIIAAG RHESPVPYAD FVTTTTHKTL RGPRGGAIIC KEKYGAALDK 250
TIFPGIQGGP LMHIIAAKAV CFGEALKDEY KEYIDQIIKN AKVFGEELVK 300
YGFRLVSGGT DNHLLLVDLT NKNITGKDLE ELLDKVNITV NKNAIPFDKL 350
KPNVTSGIRV GTPAVTTRGF KEEEMKKVAY FINKAVENRE GDLSAIKREV 400
IELCEAFPLY E 411
Length:411
Mass (Da):45,809
Last modified:July 10, 2007 - v1
Checksum:i626466A21D11E446
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000673 Genomic DNA. Translation: EDK33326.1.
RefSeqiYP_001394674.1. NC_009706.1.

Genome annotation databases

EnsemblBacteriaiEDK33326; EDK33326; CKL_1284.
GeneIDi5392545.
KEGGickl:CKL_1284.
PATRICi19464019. VBICloKlu111549_1344.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000673 Genomic DNA. Translation: EDK33326.1 .
RefSeqi YP_001394674.1. NC_009706.1.

3D structure databases

ProteinModelPortali A5N7P5.
SMRi A5N7P5. Positions 6-388.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 431943.CKL_1284.

Proteomic databases

PRIDEi A5N7P5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai EDK33326 ; EDK33326 ; CKL_1284 .
GeneIDi 5392545.
KEGGi ckl:CKL_1284.
PATRICi 19464019. VBICloKlu111549_1344.

Phylogenomic databases

eggNOGi COG0112.
HOGENOMi HOG000239404.
KOi K00600.
OMAi HLMLVDV.
OrthoDBi EOG6Z0QB2.

Enzyme and pathway databases

UniPathwayi UPA00193 .
UPA00288 ; UER01023 .
BioCyci CKLU431943:GJF1-1284-MONOMER.

Family and domain databases

Gene3Di 3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
HAMAPi MF_00051. SHMT.
InterProi IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
IPR001085. Ser_HO-MeTrfase.
IPR019798. Ser_HO-MeTrfase_PLP_BS.
[Graphical view ]
PANTHERi PTHR11680. PTHR11680. 1 hit.
Pfami PF00464. SHMT. 1 hit.
[Graphical view ]
PIRSFi PIRSF000412. SHMT. 1 hit.
SUPFAMi SSF53383. SSF53383. 1 hit.
PROSITEi PS00096. SHMT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 8527 / DSM 555 / NCIMB 10680.

Entry informationi

Entry nameiGLYA_CLOK5
AccessioniPrimary (citable) accession number: A5N7P5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: July 10, 2007
Last modified: May 14, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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