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A5N7P5

- GLYA_CLOK5

UniProt

A5N7P5 - GLYA_CLOK5

Protein

Serine hydroxymethyltransferase

Gene

glyA

Organism
Clostridium kluyveri (strain ATCC 8527 / DSM 555 / NCIMB 10680)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 55 (01 Oct 2014)
      Sequence version 1 (10 Jul 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism.UniRule annotation

    Catalytic activityi

    5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine.UniRule annotation

    Cofactori

    Pyridoxal phosphate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei33 – 331Pyridoxal phosphateUniRule annotation
    Binding sitei53 – 531Pyridoxal phosphateUniRule annotation
    Binding sitei55 – 551SubstrateUniRule annotation
    Binding sitei62 – 621SubstrateUniRule annotation
    Binding sitei63 – 631Pyridoxal phosphateUniRule annotation
    Binding sitei97 – 971Pyridoxal phosphateUniRule annotation
    Binding sitei119 – 1191Substrate; via carbonyl oxygenUniRule annotation
    Binding sitei174 – 1741Pyridoxal phosphateUniRule annotation
    Binding sitei202 – 2021Pyridoxal phosphateUniRule annotation
    Binding sitei227 – 2271Pyridoxal phosphateUniRule annotation
    Binding sitei234 – 2341Pyridoxal phosphateUniRule annotation
    Binding sitei259 – 2591Pyridoxal phosphate; via amide nitrogen and carbonyl oxygenUniRule annotation
    Binding sitei359 – 3591Pyridoxal phosphateUniRule annotation

    GO - Molecular functioni

    1. glycine hydroxymethyltransferase activity Source: UniProtKB-HAMAP
    2. pyridoxal phosphate binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. glycine biosynthetic process from serine Source: UniProtKB-HAMAP
    2. tetrahydrofolate interconversion Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Amino-acid biosynthesis, One-carbon metabolism

    Keywords - Ligandi

    Pyridoxal phosphate

    Enzyme and pathway databases

    BioCyciCKLU431943:GJF1-1284-MONOMER.
    UniPathwayiUPA00193.
    UPA00288; UER01023.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine hydroxymethyltransferaseUniRule annotation (EC:2.1.2.1UniRule annotation)
    Short name:
    SHMTUniRule annotation
    Short name:
    Serine methylaseUniRule annotation
    Gene namesi
    Name:glyAUniRule annotation
    Ordered Locus Names:CKL_1284
    OrganismiClostridium kluyveri (strain ATCC 8527 / DSM 555 / NCIMB 10680)
    Taxonomic identifieri431943 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
    ProteomesiUP000002411: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 411411Serine hydroxymethyltransferasePRO_1000074891Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei228 – 2281N6-(pyridoxal phosphate)lysineUniRule annotation

    Proteomic databases

    PRIDEiA5N7P5.

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi431943.CKL_1284.

    Structurei

    3D structure databases

    ProteinModelPortaliA5N7P5.
    SMRiA5N7P5. Positions 6-388.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni123 – 1253Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the SHMT family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0112.
    HOGENOMiHOG000239404.
    KOiK00600.
    OMAiHLMLVDV.
    OrthoDBiEOG6Z0QB2.

    Family and domain databases

    Gene3Di3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    HAMAPiMF_00051. SHMT.
    InterProiIPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    IPR001085. Ser_HO-MeTrfase.
    IPR019798. Ser_HO-MeTrfase_PLP_BS.
    [Graphical view]
    PANTHERiPTHR11680. PTHR11680. 1 hit.
    PfamiPF00464. SHMT. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000412. SHMT. 1 hit.
    SUPFAMiSSF53383. SSF53383. 1 hit.
    PROSITEiPS00096. SHMT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A5N7P5-1 [UniParc]FASTAAdd to Basket

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    MDFNELKNTD KDIYGIIEEE WERQKNGIEL IASENFTSKS VMEAMGSFLT    50
    NKYAEGYPGK RYYGGCYIVD KAEDLARDRM KKLFNAEHVN VQPHSGSQAN 100
    MAVYMSVLKP GDTVLGMSLN HGGHLTHGSK VSFSGKLYNF VSYGLNSDTE 150
    IIDYDEMREL ALKHKPKMIV SGASAYPRKI DFKKIREICD EVGAYMMVDM 200
    AHIAGIIAAG RHESPVPYAD FVTTTTHKTL RGPRGGAIIC KEKYGAALDK 250
    TIFPGIQGGP LMHIIAAKAV CFGEALKDEY KEYIDQIIKN AKVFGEELVK 300
    YGFRLVSGGT DNHLLLVDLT NKNITGKDLE ELLDKVNITV NKNAIPFDKL 350
    KPNVTSGIRV GTPAVTTRGF KEEEMKKVAY FINKAVENRE GDLSAIKREV 400
    IELCEAFPLY E 411
    Length:411
    Mass (Da):45,809
    Last modified:July 10, 2007 - v1
    Checksum:i626466A21D11E446
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000673 Genomic DNA. Translation: EDK33326.1.
    RefSeqiYP_001394674.1. NC_009706.1.

    Genome annotation databases

    EnsemblBacteriaiEDK33326; EDK33326; CKL_1284.
    GeneIDi5392545.
    KEGGickl:CKL_1284.
    PATRICi19464019. VBICloKlu111549_1344.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000673 Genomic DNA. Translation: EDK33326.1 .
    RefSeqi YP_001394674.1. NC_009706.1.

    3D structure databases

    ProteinModelPortali A5N7P5.
    SMRi A5N7P5. Positions 6-388.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 431943.CKL_1284.

    Proteomic databases

    PRIDEi A5N7P5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai EDK33326 ; EDK33326 ; CKL_1284 .
    GeneIDi 5392545.
    KEGGi ckl:CKL_1284.
    PATRICi 19464019. VBICloKlu111549_1344.

    Phylogenomic databases

    eggNOGi COG0112.
    HOGENOMi HOG000239404.
    KOi K00600.
    OMAi HLMLVDV.
    OrthoDBi EOG6Z0QB2.

    Enzyme and pathway databases

    UniPathwayi UPA00193 .
    UPA00288 ; UER01023 .
    BioCyci CKLU431943:GJF1-1284-MONOMER.

    Family and domain databases

    Gene3Di 3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    HAMAPi MF_00051. SHMT.
    InterProi IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    IPR001085. Ser_HO-MeTrfase.
    IPR019798. Ser_HO-MeTrfase_PLP_BS.
    [Graphical view ]
    PANTHERi PTHR11680. PTHR11680. 1 hit.
    Pfami PF00464. SHMT. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000412. SHMT. 1 hit.
    SUPFAMi SSF53383. SSF53383. 1 hit.
    PROSITEi PS00096. SHMT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 8527 / DSM 555 / NCIMB 10680.

    Entry informationi

    Entry nameiGLYA_CLOK5
    AccessioniPrimary (citable) accession number: A5N7P5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 20, 2008
    Last sequence update: July 10, 2007
    Last modified: October 1, 2014
    This is version 55 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3