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A5N6Y3 (SYQ_CLOK5) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 51. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamine--tRNA ligase

EC=6.1.1.18
Alternative name(s):
Glutaminyl-tRNA synthetase
Short name=GlnRS
Gene names
Name:glnS
Ordered Locus Names:CKL_1022
OrganismClostridium kluyveri (strain ATCC 8527 / DSM 555 / NCIMB 10680) [Complete proteome] [HAMAP]
Taxonomic identifier431943 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length556 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln). HAMAP-Rule MF_00126

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00126

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00126.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processglutaminyl-tRNA aminoacylation

Inferred from electronic annotation. Source: InterPro

glutamyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

glutamine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 556556Glutamine--tRNA ligase HAMAP-Rule MF_00126
PRO_1000095483

Regions

Motif33 – 4311"HIGH" region HAMAP-Rule MF_00126
Motif266 – 2705"KMSKS" region HAMAP-Rule MF_00126

Sites

Binding site2691ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A5N6Y3 [UniParc].

Last modified July 10, 2007. Version 1.
Checksum: F54B497C7FBED2F5

FASTA55664,440
        10         20         30         40         50         60 
MGDNNNSSNF IKNIIIEDLK SGEYEKVVTR FPPEPNGYLH IGHAKSIVLN FSLADEFEGK 

        70         80         90        100        110        120 
VNLRFDDTNP SKEDVEYVES IKEDVKWLGY NWDNLCFASS YFERMYEYAV FLIKKGKAYV 

       130        140        150        160        170        180 
CDLTADEIRE YRGTLTKPGK ESPYRNRDVE ENFKLFEGMK NGEFKDGEKV LRAKIDMASP 

       190        200        210        220        230        240 
NINMRDPVLY RIAHASHHNT GDRWCIYPMY DFAHPLEDAI EGVTHSICTL EFEDHRPLYD 

       250        260        270        280        290        300 
WIIQECEIEN RPKQIEFARL NMTNTVMSKR KLKQLVDENF VDGWDDPRMP TIAGLRRRGF 

       310        320        330        340        350        360 
TPESIRNFCR AIGVSKASSI VDSQMLDYFL RDDLEKEAPR TMAILNPLKV VITNYPEGKT 

       370        380        390        400        410        420 
ETFKIENNPD DASAGVREVP FSREIYIEQE DFMENPPKKY YRLFPGNEVR LKSAYFIKCT 

       430        440        450        460        470        480 
EAIKDKDGNV IELRCTYDPA SKGGNSPDGR KVRGTLHWIN VDTAIPAEIR LYEPLILEQQ 

       490        500        510        520        530        540 
EKEGEEDLFL DHVNPNSLKV VKGFVEPDMK HVKSDEKFQF FRHGYFVLDG KCSKNGELVF 

       550 
NRIVSLKSSF KIPENK 

« Hide

References

[1]"The genome of Clostridium kluyveri, a strict anaerobe with unique metabolic features."
Seedorf H., Fricke W.F., Veith B., Brueggemann H., Liesegang H., Strittmatter A., Miethke M., Buckel W., Hinderberger J., Li F., Hagemeier C., Thauer R.K., Gottschalk G.
Proc. Natl. Acad. Sci. U.S.A. 105:2128-2133(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 8527 / DSM 555 / NCIMB 10680.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000673 Genomic DNA. Translation: EDK33064.1.
RefSeqYP_001394412.1. NC_009706.1.

3D structure databases

ProteinModelPortalA5N6Y3.
SMRA5N6Y3. Positions 8-549.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING431943.CKL_1022.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEDK33064; EDK33064; CKL_1022.
GeneID5392980.
KEGGckl:CKL_1022.
PATRIC19463487. VBICloKlu111549_1078.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0008.
HOGENOMHOG000259232.
KOK01886.
OMAVTHSICT.
OrthoDBEOG6DRPF7.
ProtClustDBPRK05347.

Enzyme and pathway databases

BioCycCKLU431943:GJF1-1022-MONOMER.

Family and domain databases

Gene3D1.10.1160.10. 1 hit.
2.40.240.10. 2 hits.
3.40.50.620. 2 hits.
HAMAPMF_00126. Gln_tRNA_synth.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR004514. Gln-tRNA-synth.
IPR022861. Gln_tRNA_ligase_bac.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl.
IPR011035. Ribosomal_L25/Gln-tRNA_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERPTHR10119. PTHR10119. 1 hit.
PfamPF00749. tRNA-synt_1c. 1 hit.
PF03950. tRNA-synt_1c_C. 1 hit.
[Graphical view]
PRINTSPR00987. TRNASYNTHGLU.
SUPFAMSSF50715. SSF50715. 1 hit.
TIGRFAMsTIGR00440. glnS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYQ_CLOK5
AccessionPrimary (citable) accession number: A5N6Y3
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: July 10, 2007
Last modified: February 19, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries