A5N6Y3 (SYQ_CLOK5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamine--tRNA ligase
EC=6.1.1.18

Alternative name(s):
Glutaminyl-tRNA synthetase
Short name=GlnRS

Gene names

Name:	glnS
Ordered Locus Names:	CKL_1022

Organism

Clostridium kluyveri (strain ATCC 8527 / DSM 555 / NCIMB 10680) [Complete proteome] [HAMAP]

Taxonomic identifier

431943 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	556 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln). HAMAP MF_00126
Subunit structure	Monomer By similarity. HAMAP MF_00126
Subcellular location	Cytoplasm By similarity HAMAP MF_00126.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutaminyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 556	556	Glutamine--tRNA ligase HAMAP MF_00126		PRO_1000095483
Regions
	Motif	33 – 43	11	"HIGH" region HAMAP MF_00126
	Motif	266 – 270	5	"KMSKS" region HAMAP MF_00126
Sites
	Binding site	269	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5N6Y3 [UniParc]. Last modified July 10, 2007. Version 1. Checksum: F54B497C7FBED2F5	FASTA	556	64,440
	10 20 30 40 50 60 MGDNNNSSNF IKNIIIEDLK SGEYEKVVTR FPPEPNGYLH IGHAKSIVLN FSLADEFEGK 70 80 90 100 110 120 VNLRFDDTNP SKEDVEYVES IKEDVKWLGY NWDNLCFASS YFERMYEYAV FLIKKGKAYV 130 140 150 160 170 180 CDLTADEIRE YRGTLTKPGK ESPYRNRDVE ENFKLFEGMK NGEFKDGEKV LRAKIDMASP 190 200 210 220 230 240 NINMRDPVLY RIAHASHHNT GDRWCIYPMY DFAHPLEDAI EGVTHSICTL EFEDHRPLYD 250 260 270 280 290 300 WIIQECEIEN RPKQIEFARL NMTNTVMSKR KLKQLVDENF VDGWDDPRMP TIAGLRRRGF 310 320 330 340 350 360 TPESIRNFCR AIGVSKASSI VDSQMLDYFL RDDLEKEAPR TMAILNPLKV VITNYPEGKT 370 380 390 400 410 420 ETFKIENNPD DASAGVREVP FSREIYIEQE DFMENPPKKY YRLFPGNEVR LKSAYFIKCT 430 440 450 460 470 480 EAIKDKDGNV IELRCTYDPA SKGGNSPDGR KVRGTLHWIN VDTAIPAEIR LYEPLILEQQ 490 500 510 520 530 540 EKEGEEDLFL DHVNPNSLKV VKGFVEPDMK HVKSDEKFQF FRHGYFVLDG KCSKNGELVF 550 NRIVSLKSSF KIPENK

References

[1]

"The genome of Clostridium kluyveri, a strict anaerobe with unique metabolic features." Seedorf H., Fricke W.F., Veith B., Brueggemann H., Liesegang H., Strittmatter A., Miethke M., Buckel W., Hinderberger J., Li F., Hagemeier C., Thauer R.K., Gottschalk G. Proc. Natl. Acad. Sci. U.S.A. 105:2128-2133(2008) [PubMed: 18218779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 8527 / DSM 555 / NCIMB 10680.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000673 Genomic DNA. Translation: EDK33064.1.
RefSeq	YP_001394412.1. NC_009706.1.
3D structure databases
ProteinModelPortal	A5N6Y3.
SMR	A5N6Y3. Positions 8-549.
ModBase	Search...
Protein-protein interaction databases
STRING	A5N6Y3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5392980.
GenomeReviews	Gene locus CKL_1022 in contig CP000673_GR.
KEGG	ckl:CKL_1022.
NMPDR	fig|431943.4.peg.1065.
PATRIC	19463487. VBICloKlu111549_1078.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0008.
HOGENOM	HBG334108.
OMA	RMPTIAG.
ProtClustDB	PRK05347.
Enzyme and pathway databases
BioCyc	CKLU431943:CKL_1022-MONOMER.
Family and domain databases
HAMAP	MF_00126. Gln_tRNA_synth. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR004514. Gln-tRNA-synth_Ib. IPR022861. Gln_tRNA_synth_bac. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd. IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl. IPR011035. Ribosomal_L25/Gln-tRNA_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:2.40.240.10. Rbsml_L25/Gln-tRNA_synth_b-brl. 2 hits. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KO	K01886.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. PF03950. tRNA-synt_1c_C. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF50715. Ribosomal_L25rel. 1 hit.
TIGRFAMs	TIGR00440. GlnS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYQ_CLOK5

Accession

Primary (citable) accession number: A5N6Y3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	July 10, 2007
Last modified:	January 25, 2012
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program