A5N6W8 (AROD_CLOK5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-dehydroquinate dehydratase
Short name=3-dehydroquinase
EC=4.2.1.10

Alternative name(s):
Type I DHQase

Gene names

Name:	aroD
Ordered Locus Names:	CKL_1007

Organism

Clostridium kluyveri (strain ATCC 8527 / DSM 555 / NCIMB 10680) [Complete proteome] [HAMAP]

Taxonomic identifier

431943 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	255 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	3-dehydroquinate = 3-dehydroshikimate + H2O. HAMAP MF_00214
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 3/7. HAMAP MF_00214
Subunit structure	Homodimer By similarity. HAMAP MF_00214
Sequence similarities	Belongs to the type-I 3-dehydroquinase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Ligand	Schiff base
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3-dehydroquinate dehydratase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 255	255	3-dehydroquinate dehydratase HAMAP MF_00214		PRO_1000078042
Sites
	Active site	145	1	Proton acceptor By similarity
	Active site	172	1	Schiff-base intermediate with substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5N6W8 [UniParc]. Last modified July 10, 2007. Version 1. Checksum: 8891A6C9FE44915F	FASTA	255	28,536
	10 20 30 40 50 60 MGSIVKIRDV KLGEGIPKIA VPLVGSNEEE IMEEIAGVKT TKLDIVEWRI DYYKYVEEVE 70 80 90 100 110 120 KVKKLLQKMR KNLNNIPILV TFRTAKEGGK REISLEYYIE LNKAIAATGN TDMIDIELFA 130 140 150 160 170 180 AEDEAVKKIV EELHEYNIKV IMSNHDFHKT PHKDELISRM CRMQQLGADI AKIAVMPCST 190 200 210 220 230 240 KDVLELLSAT CEMKCKHNDT PIITMSMGTL GVITRLAGET FGSALTFGSA KAASAPGQLE 250 VNELYKVLKL ISAYR

References

[1]

"The genome of Clostridium kluyveri, a strict anaerobe with unique metabolic features." Seedorf H., Fricke W.F., Veith B., Brueggemann H., Liesegang H., Strittmatter A., Miethke M., Buckel W., Hinderberger J., Li F., Hagemeier C., Thauer R.K., Gottschalk G. Proc. Natl. Acad. Sci. U.S.A. 105:2128-2133(2008) [PubMed: 18218779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 8527 / DSM 555 / NCIMB 10680.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000673 Genomic DNA. Translation: EDK33049.1.
RefSeq	YP_001394397.1. NC_009706.1.
3D structure databases
ProteinModelPortal	A5N6W8.
ModBase	Search...
Protein-protein interaction databases
STRING	A5N6W8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5391588.
GenomeReviews	Gene locus CKL_1007 in contig CP000673_GR.
KEGG	ckl:CKL_1007.
NMPDR	fig|431943.4.peg.1050.
PATRIC	19463457. VBICloKlu111549_1063.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0710.
HOGENOM	HBG732926.
OMA	IAKIAVM.
ProtClustDB	CLSK2474778.
Enzyme and pathway databases
BioCyc	CKLU431943:CKL_1007-MONOMER.
Family and domain databases
HAMAP	MF_00214. AroD. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR001381. DHquinase_I. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K03785.
Pfam	PF01487. DHquinase_I. 1 hit. [Graphical view]
TIGRFAMs	TIGR01093. AroD. 1 hit.
PROSITE	PS01028. DEHYDROQUINASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROD_CLOK5

Accession

Primary (citable) accession number: A5N6W8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	July 10, 2007
Last modified:	December 14, 2011
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program