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Protein

Argininosuccinate synthase

Gene

argG

Organism
Clostridium kluyveri (strain ATCC 8527 / DSM 555 / NCIMB 10680)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.UniRule annotation

Pathway: L-arginine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Argininosuccinate synthase (argG)
  3. Argininosuccinate lyase (argH)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei86 – 861CitrullineUniRule annotation
Binding sitei91 – 911CitrullineUniRule annotation
Binding sitei116 – 1161ATP; via amide nitrogenUniRule annotation
Binding sitei118 – 1181AspartateUniRule annotation
Binding sitei122 – 1221AspartateUniRule annotation
Binding sitei122 – 1221CitrullineUniRule annotation
Binding sitei123 – 1231AspartateUniRule annotation
Binding sitei126 – 1261CitrullineUniRule annotation
Binding sitei175 – 1751CitrullineUniRule annotation
Binding sitei184 – 1841CitrullineUniRule annotation
Binding sitei260 – 2601CitrullineUniRule annotation
Binding sitei272 – 2721CitrullineUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi8 – 169ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCKLU431943:GJF1-981-MONOMER.
UniPathwayiUPA00068; UER00113.

Names & Taxonomyi

Protein namesi
Recommended name:
Argininosuccinate synthaseUniRule annotation (EC:6.3.4.5UniRule annotation)
Alternative name(s):
Citrulline--aspartate ligaseUniRule annotation
Gene namesi
Name:argGUniRule annotation
Ordered Locus Names:CKL_0981
OrganismiClostridium kluyveri (strain ATCC 8527 / DSM 555 / NCIMB 10680)
Taxonomic identifieri431943 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
ProteomesiUP000002411 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 401401Argininosuccinate synthasePRO_1000073817Add
BLAST

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi431943.CKL_0981.

Structurei

3D structure databases

ProteinModelPortaliA5N6U2.
SMRiA5N6U2. Positions 4-397.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the argininosuccinate synthase family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0137.
HOGENOMiHOG000230093.
KOiK01940.
OMAiPAREWGM.
OrthoDBiEOG6K9QCV.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A5N6U2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKDKVVLAYS GGLDTSIIIP WLKENYDLDV IAACIDVGQD DDMEAVRNKA
60 70 80 90 100
IKTGAVKVYI EDVKEEFVRD YLFSAVKAHI LYEDAYLLGT SLARPLMAKR
110 120 130 140 150
LVEIAHAEGA KYIAHGCTGK GNDQVRFEVG VASFDPKLGI IAPWRIWDIK
160 170 180 190 200
SREDAIDYAN SKGVEVPVTK EKIYSNDKNI WHVSHEGGDL EDPKNEHKSS
210 220 230 240 250
MYFMTTPPEK AKDEVSYVEL YFEQGIPKKL DGKELPPVEM MQTLNKLGGE
260 270 280 290 300
NGIGIVDMVE NRLVGMKSRG VYETPGGTIL YAAHAALERL TIDKNTAHYK
310 320 330 340 350
QMISQKYGEL VYDGLWFSPL KEALDAFVEV TQKNVTGSVK LKLYKGNVMV
360 370 380 390 400
AGVDAPYALY DEDISSFGAS ELYDHKDAEG FIKIFSLPYK IKAMIEKEKG

N
Length:401
Mass (Da):44,802
Last modified:July 10, 2007 - v1
Checksum:i9F3032FA39B64220
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000673 Genomic DNA. Translation: EDK33023.1.
RefSeqiWP_012101353.1. NC_009706.1.
YP_001394371.1. NC_009706.1.

Genome annotation databases

EnsemblBacteriaiEDK33023; EDK33023; CKL_0981.
KEGGickl:CKL_0981.
PATRICi19463405. VBICloKlu111549_1037.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000673 Genomic DNA. Translation: EDK33023.1.
RefSeqiWP_012101353.1. NC_009706.1.
YP_001394371.1. NC_009706.1.

3D structure databases

ProteinModelPortaliA5N6U2.
SMRiA5N6U2. Positions 4-397.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi431943.CKL_0981.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiEDK33023; EDK33023; CKL_0981.
KEGGickl:CKL_0981.
PATRICi19463405. VBICloKlu111549_1037.

Phylogenomic databases

eggNOGiCOG0137.
HOGENOMiHOG000230093.
KOiK01940.
OMAiPAREWGM.
OrthoDBiEOG6K9QCV.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113.
BioCyciCKLU431943:GJF1-981-MONOMER.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 8527 / DSM 555 / NCIMB 10680.

Entry informationi

Entry nameiASSY_CLOK5
AccessioniPrimary (citable) accession number: A5N6U2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: July 10, 2007
Last modified: April 29, 2015
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.