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Protein

Isocitrate dehydrogenase [NADP]

Gene

idh

Organism
Clostridium kluyveri (strain ATCC 8527 / DSM 555 / NCIMB 10680)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH.UniRule annotation

Cofactori

Mg2+UniRule annotation, Mn2+UniRule annotationNote: Binds 1 Mg(2+) or Mn2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei78 – 781SubstrateUniRule annotation
Binding sitei110 – 1101SubstrateUniRule annotation
Binding sitei133 – 1331SubstrateUniRule annotation
Sitei140 – 1401Critical for catalysisUniRule annotation
Sitei211 – 2111Critical for catalysisUniRule annotation
Metal bindingi251 – 2511Magnesium or manganeseUniRule annotation
Metal bindingi274 – 2741Magnesium or manganeseUniRule annotation

GO - Molecular functioni

  1. isocitrate dehydrogenase (NADP+) activity Source: UniProtKB-EC
  2. magnesium ion binding Source: InterPro
  3. NAD binding Source: InterPro

GO - Biological processi

  1. isocitrate metabolic process Source: InterPro
  2. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

OxidoreductaseUniRule annotation

Keywords - Biological processi

Tricarboxylic acid cycleUniRule annotation

Keywords - Ligandi

MagnesiumUniRule annotation, ManganeseUniRule annotation, Metal-bindingUniRule annotation, NADPUniRule annotation

Enzyme and pathway databases

BioCyciCKLU431943:GJF1-546-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isocitrate dehydrogenase [NADP]UniRule annotation (EC:1.1.1.42UniRule annotation)
Gene namesi
Name:idhImported
Ordered Locus Names:CKL_0546Imported
OrganismiClostridium kluyveri (strain ATCC 8527 / DSM 555 / NCIMB 10680)Imported
Taxonomic identifieri431943 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
ProteomesiUP000002411 Componenti: Chromosome

Interactioni

Protein-protein interaction databases

STRINGi431943.CKL_0546.

Structurei

3D structure databases

ProteinModelPortaliA5N5L9.
SMRiA5N5L9. Positions 4-401.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni95 – 1017Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the isocitrate and isopropylmalate dehydrogenases family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0538.
HOGENOMiHOG000019858.
KOiK00031.
OMAiDVMSDMI.
OrthoDBiEOG628F3X.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR004790. Isocitrate_DH_NADP.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERiPTHR11822. PTHR11822. 1 hit.
PfamiPF00180. Iso_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF000108. IDH_NADP. 1 hit.
TIGRFAMsiTIGR00127. nadp_idh_euk. 1 hit.
PROSITEiPS00470. IDH_IMDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A5N5L9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEKIKMNVP LVEMDGDEMT RIIWKMIKEL LLEPYIDLKT EYYDLGLVKR
60 70 80 90 100
NETNDEITIE AANAIKKYGV GVKCATITPN AKRVKEYNLK SMWKSPNGTI
110 120 130 140 150
RAILDGTVFR TPIIVNSIRP LMRTWEKPIT VARHAYGDVY RDVEYKVEEP
160 170 180 190 200
GKMELVFTSE KGEETRQTLH VFNGPGVVMG MHNLDKSIES FARSCFNYAL
210 220 230 240 250
DMNQNLWFAS KDTISKTYDH RFKDIFQEIY DTEYDAKFND AGIEYFYTLI
260 270 280 290 300
DDAVARVVKS EGGFIWACKN YDGDVMSDMV ATAFGSLAMM TSVLVSPEGY
310 320 330 340 350
YEYEAAHGTV QKHYYQHLKG QLTSTNSMAT LFAWTGALRK RGEIDGINEL
360 370 380 390 400
VEFADRLENT SIRTIEEGIM TKDLASLSEL ENKKIVNTEE FLLEIKKRLE

E
Length:401
Mass (Da):45,998
Last modified:July 9, 2007 - v1
Checksum:i7D457928FFA7FF20
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000673 Genomic DNA. Translation: EDK32600.1.
RefSeqiYP_001393948.1. NC_009706.1.

Genome annotation databases

EnsemblBacteriaiEDK32600; EDK32600; CKL_0546.
KEGGickl:CKL_0546.
PATRICi19462511. VBICloKlu111549_0602.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000673 Genomic DNA. Translation: EDK32600.1.
RefSeqiYP_001393948.1. NC_009706.1.

3D structure databases

ProteinModelPortaliA5N5L9.
SMRiA5N5L9. Positions 4-401.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi431943.CKL_0546.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiEDK32600; EDK32600; CKL_0546.
KEGGickl:CKL_0546.
PATRICi19462511. VBICloKlu111549_0602.

Phylogenomic databases

eggNOGiCOG0538.
HOGENOMiHOG000019858.
KOiK00031.
OMAiDVMSDMI.
OrthoDBiEOG628F3X.

Enzyme and pathway databases

BioCyciCKLU431943:GJF1-546-MONOMER.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR004790. Isocitrate_DH_NADP.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERiPTHR11822. PTHR11822. 1 hit.
PfamiPF00180. Iso_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF000108. IDH_NADP. 1 hit.
TIGRFAMsiTIGR00127. nadp_idh_euk. 1 hit.
PROSITEiPS00470. IDH_IMDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 8527 / DSM 555 / NCIMB 10680Imported.

Entry informationi

Entry nameiA5N5L9_CLOK5
AccessioniPrimary (citable) accession number: A5N5L9
Entry historyi
Integrated into UniProtKB/TrEMBL: July 9, 2007
Last sequence update: July 9, 2007
Last modified: March 31, 2015
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.