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Protein

Formate--tetrahydrofolate ligase

Gene

fhs

Organism
Clostridium kluyveri (strain ATCC 8527 / DSM 555 / NCIMB 10680)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate.UniRule annotation

Pathway:itetrahydrofolate interconversion

This protein is involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway tetrahydrofolate interconversion and in One-carbon metabolism.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi66 – 738ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

One-carbon metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCKLU431943:GJF1-440-MONOMER.
UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
Formate--tetrahydrofolate ligaseUniRule annotation (EC:6.3.4.3UniRule annotation)
Alternative name(s):
Formyltetrahydrofolate synthetaseUniRule annotation
Short name:
FHSUniRule annotation
Short name:
FTHFSUniRule annotation
Gene namesi
Name:fhsUniRule annotation
Ordered Locus Names:CKL_0440
OrganismiClostridium kluyveri (strain ATCC 8527 / DSM 555 / NCIMB 10680)
Taxonomic identifieri431943 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
ProteomesiUP000002411 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 558558Formate--tetrahydrofolate ligasePRO_1000087651Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi431943.CKL_0440.

Structurei

3D structure databases

ProteinModelPortaliA5N5B3.
SMRiA5N5B3. Positions 6-556.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the formate--tetrahydrofolate ligase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG2759.
HOGENOMiHOG000040280.
KOiK01938.
OMAiIANSVSM.
OrthoDBiEOG6PCPSP.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_01543. FTHFS.
InterProiIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01268. FTHFS. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A5N5B3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNFKSDIQIA QECSMENIKD IAKKLNIFED EIELYGKYKA KIDYNLLKTT
60 70 80 90 100
KGKNGKLILC TAINPTPAGE GKTTTSIGVA DALARLDKSV VVALREPSMG
110 120 130 140 150
PVFGIKGGAA GGGYAQVVPM EDINLHFTGD IHAMTAANNL LAALIDNHIY
160 170 180 190 200
QGNKLNIDSR RVVWRRCVDM NDRQLRFVVD GLGGKVNGIP REDGFDITVA
210 220 230 240 250
SEIMAIFCLS TDINDLKERI SKIVVGYTTE GNPVTAHDLK AEGAMAALLK
260 270 280 290 300
DALKPNLVQT LEGTPAFVHG GPFANIAHGC NSIMATRMAM HFGDYVVTEA
310 320 330 340 350
GFGADLGAEK FLDIKCRMAG LRPDAVIIVA TVRALKYNGG TPKTKLNNEN
360 370 380 390 400
LETLEKGIPN LLKHVENITK VFKLPAVVAL NAFPTDTEAE LKLVEEKCRE
410 420 430 440 450
FGVSVKLSEV WAKGGEGGIE VAKEVLRLIN EGKNDFQFAY DEKLPIRDKI
460 470 480 490 500
RAIAQKIYGA DGVTFTNQAE KEIDELEKLG FGKTPVCIAK TQYSLTDDQN
510 520 530 540 550
KLGRPKGFKI TVRQVSISAG AGFVVAITGS IMKMPGLPKV PAAEKIDVDE

NGVISGLF
Length:558
Mass (Da):60,414
Last modified:July 10, 2007 - v1
Checksum:i6E7ABE3A88C40AE8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000673 Genomic DNA. Translation: EDK32494.1.
RefSeqiWP_011989009.1. NC_009706.1.

Genome annotation databases

EnsemblBacteriaiEDK32494; EDK32494; CKL_0440.
KEGGickl:CKL_0440.
PATRICi19462281. VBICloKlu111549_0487.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000673 Genomic DNA. Translation: EDK32494.1.
RefSeqiWP_011989009.1. NC_009706.1.

3D structure databases

ProteinModelPortaliA5N5B3.
SMRiA5N5B3. Positions 6-556.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi431943.CKL_0440.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiEDK32494; EDK32494; CKL_0440.
KEGGickl:CKL_0440.
PATRICi19462281. VBICloKlu111549_0487.

Phylogenomic databases

eggNOGiCOG2759.
HOGENOMiHOG000040280.
KOiK01938.
OMAiIANSVSM.
OrthoDBiEOG6PCPSP.

Enzyme and pathway databases

UniPathwayiUPA00193.
BioCyciCKLU431943:GJF1-440-MONOMER.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_01543. FTHFS.
InterProiIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01268. FTHFS. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 8527 / DSM 555 / NCIMB 10680.

Entry informationi

Entry nameiFTHS_CLOK5
AccessioniPrimary (citable) accession number: A5N5B3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: July 10, 2007
Last modified: July 22, 2015
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.