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A5N5B3 (FTHS_CLOK5) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 46. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Formate--tetrahydrofolate ligase

EC=6.3.4.3
Alternative name(s):
Formyltetrahydrofolate synthetase
Short name=FHS
Short name=FTHFS
Gene names
Name:fhs
Ordered Locus Names:CKL_0440
OrganismClostridium kluyveri (strain ATCC 8527 / DSM 555 / NCIMB 10680) [Complete proteome] [HAMAP]
Taxonomic identifier431943 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length558 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate. HAMAP-Rule MF_01543

Pathway

One-carbon metabolism; tetrahydrofolate interconversion. HAMAP-Rule MF_01543

Sequence similarities

Belongs to the formate--tetrahydrofolate ligase family.

Ontologies

Keywords
   Biological processOne-carbon metabolism
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processfolic acid-containing compound biosynthetic process

Inferred from electronic annotation. Source: InterPro

tetrahydrofolate interconversion

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

formate-tetrahydrofolate ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 558558Formate--tetrahydrofolate ligase HAMAP-Rule MF_01543
PRO_1000087651

Regions

Nucleotide binding66 – 738ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A5N5B3 [UniParc].

Last modified July 10, 2007. Version 1.
Checksum: 6E7ABE3A88C40AE8

FASTA55860,414
        10         20         30         40         50         60 
MNFKSDIQIA QECSMENIKD IAKKLNIFED EIELYGKYKA KIDYNLLKTT KGKNGKLILC 

        70         80         90        100        110        120 
TAINPTPAGE GKTTTSIGVA DALARLDKSV VVALREPSMG PVFGIKGGAA GGGYAQVVPM 

       130        140        150        160        170        180 
EDINLHFTGD IHAMTAANNL LAALIDNHIY QGNKLNIDSR RVVWRRCVDM NDRQLRFVVD 

       190        200        210        220        230        240 
GLGGKVNGIP REDGFDITVA SEIMAIFCLS TDINDLKERI SKIVVGYTTE GNPVTAHDLK 

       250        260        270        280        290        300 
AEGAMAALLK DALKPNLVQT LEGTPAFVHG GPFANIAHGC NSIMATRMAM HFGDYVVTEA 

       310        320        330        340        350        360 
GFGADLGAEK FLDIKCRMAG LRPDAVIIVA TVRALKYNGG TPKTKLNNEN LETLEKGIPN 

       370        380        390        400        410        420 
LLKHVENITK VFKLPAVVAL NAFPTDTEAE LKLVEEKCRE FGVSVKLSEV WAKGGEGGIE 

       430        440        450        460        470        480 
VAKEVLRLIN EGKNDFQFAY DEKLPIRDKI RAIAQKIYGA DGVTFTNQAE KEIDELEKLG 

       490        500        510        520        530        540 
FGKTPVCIAK TQYSLTDDQN KLGRPKGFKI TVRQVSISAG AGFVVAITGS IMKMPGLPKV 

       550 
PAAEKIDVDE NGVISGLF 

« Hide

References

[1]"The genome of Clostridium kluyveri, a strict anaerobe with unique metabolic features."
Seedorf H., Fricke W.F., Veith B., Brueggemann H., Liesegang H., Strittmatter A., Miethke M., Buckel W., Hinderberger J., Li F., Hagemeier C., Thauer R.K., Gottschalk G.
Proc. Natl. Acad. Sci. U.S.A. 105:2128-2133(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 8527 / DSM 555 / NCIMB 10680.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000673 Genomic DNA. Translation: EDK32494.1.
RefSeqYP_001393842.1. NC_009706.1.

3D structure databases

ProteinModelPortalA5N5B3.
SMRA5N5B3. Positions 6-556.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING431943.CKL_0440.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEDK32494; EDK32494; CKL_0440.
GeneID5391110.
KEGGckl:CKL_0440.
PATRIC19462281. VBICloKlu111549_0487.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2759.
HOGENOMHOG000040280.
KOK01938.
OMAIANSVSM.
OrthoDBEOG6PCPSP.

Enzyme and pathway databases

BioCycCKLU431943:GJF1-440-MONOMER.
UniPathwayUPA00193.

Family and domain databases

Gene3D3.40.50.300. 2 hits.
HAMAPMF_01543. FTHFS.
InterProIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamPF01268. FTHFS. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
PROSITEPS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameFTHS_CLOK5
AccessionPrimary (citable) accession number: A5N5B3
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: July 10, 2007
Last modified: May 14, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways