A5N4P4 (EFG_CLOK5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Elongation factor G
Short name=EF-G

Gene names

Name:	fusA
Ordered Locus Names:	CKL_0221

Organism

Clostridium kluyveri (strain ATCC 8527 / DSM 555 / NCIMB 10680) [Complete proteome] [HAMAP]

Taxonomic identifier

431943 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	688 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome By similarity. HAMAP MF_00054_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00054_B.
Sequence similarities	Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	GTP-binding Nucleotide-binding
Molecular function	Elongation factor
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Inferred from electronic annotation. Source: InterPro translation elongation factor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 688	688	Elongation factor G HAMAP MF_00054_B		PRO_1000074953
Regions
	Nucleotide binding	17 – 24	8	GTP By similarity
	Nucleotide binding	81 – 85	5	GTP By similarity
	Nucleotide binding	135 – 138	4	GTP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5N4P4 [UniParc]. Last modified July 10, 2007. Version 1. Checksum: FCA3D3D5019B08A6	FASTA	688	76,491
	10 20 30 40 50 60 MGREYPLEKF RNIGIMAHID AGKTTTTERI LFYTGRTHKI GEVHEGQATM DWMAQEQERG 70 80 90 100 110 120 ITITSAATFC KWKGYAINII DTPGHVDFTV EVERSLRVLD GAVTVLDAKS GVEPQTETVW 130 140 150 160 170 180 RQADNYGVPR LVYVNKMDST GADFYMCVNT LRDRLHCNAI PIQIPIGSES EFKGIVNLVK 190 200 210 220 230 240 NEAIIYEDDL GTVMDEVEIP GDLKDEAEKY RTELIEAISE LDEDIMMKYL EGEELTEEEI 250 260 270 280 290 300 ISAIRKGVIS NKIVPVLCGS SYKNKGVQPM IDAVVDFMPS PLDIPPIKGT DPETGEETDR 310 320 330 340 350 360 PADDNQPLSA LAFKIATDPF VGKLAFTRIY SGIMKSGTYV YNSTKGKKER IARLVKMHSN 370 380 390 400 410 420 RREEVDELRA GDLGAIVGLK DTTTGNTLCD EQNAVVLESM EFPEPVIHVA IEPKTKAGQE 430 440 450 460 470 480 KMGIALSKLA EEDPTFKTHT DQETGQTIIS GMGELHLEII VDRLQREFKV ECNVGKPQVA 490 500 510 520 530 540 YKETIRKTVK AEGKFVRQSG GHGQYGHCWI EMSPSEEGYS FENAIVGGTI PKEYISPIDE 550 560 570 580 590 600 GIKQASETGT VAGYPAINFK VKLYDGSYHD VDSSEMAFKI AASMAFKNAM SKADPVLLEP 610 620 630 640 650 660 MMRVEVTVPE EYMGDVIGDI NSRRGRIEGM DPRAGAQVIR SFVPLSEMFG YATVLRSRTQ 670 680 GRGVYSMTFD HYEEVPKSIQ EKITGEKK

References

[1]

"The genome of Clostridium kluyveri, a strict anaerobe with unique metabolic features." Seedorf H., Fricke W.F., Veith B., Brueggemann H., Liesegang H., Strittmatter A., Miethke M., Buckel W., Hinderberger J., Li F., Hagemeier C., Thauer R.K., Gottschalk G. Proc. Natl. Acad. Sci. U.S.A. 105:2128-2133(2008) [PubMed: 18218779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 8527 / DSM 555 / NCIMB 10680.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000673 Genomic DNA. Translation: EDK32275.1.
RefSeq	YP_001393623.1. NC_009706.1.
3D structure databases
ProteinModelPortal	A5N4P4.
SMR	A5N4P4. Positions 4-683.
ModBase	Search...
Protein-protein interaction databases
STRING	A5N4P4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5392582.
GenomeReviews	Gene locus CKL_0221 in contig CP000673_GR.
KEGG	ckl:CKL_0221.
NMPDR	fig|431943.4.peg.257.
PATRIC	19461845. VBICloKlu111549_0269.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0480.
HOGENOM	HBG737692.
OMA	CNEWREK.
ProtClustDB	PRK00007.
Enzyme and pathway databases
BioCyc	CKLU431943:CKL_0221-MONOMER.
Family and domain databases
HAMAP	MF_00054_B. EF-G_EF-2_B. [Tree]
InterPro	IPR009022. Elongation_fac_G/III/V. IPR000795. ProtSyn_GTP-bd. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. IPR005225. Small_GTP-bd_dom. IPR004540. Transl_elong_EFG/EF2. IPR000640. Transl_elong_EFG/EF2_C. IPR005517. Transl_elong_EFG/EF2_IV. IPR004161. Transl_elong_EFTu/EF1A_2. IPR009000. Transl_elong_init/rib_B-barrel. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit. G3DSA:3.30.70.240. Transl_elong_EFG/EF2_C. 1 hit.
KO	K02355.
Pfam	PF00679. EFG_C. 1 hit. PF03764. EFG_IV. 1 hit. PF00009. GTP_EFTU. 1 hit. PF03144. GTP_EFTU_D2. 1 hit. [Graphical view]
PRINTS	PR00315. ELONGATNFCT.
SMART	SM00838. EFG_C. 1 hit. SM00889. EFG_IV. 1 hit. [Graphical view]
SUPFAM	SSF54980. EFG_III_V. 2 hits. SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit. SSF50447. Translat_factor. 1 hit.
TIGRFAMs	TIGR00484. EF-G. 1 hit. TIGR00231. Small_GTP. 1 hit.
PROSITE	PS00301. EFACTOR_GTP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EFG_CLOK5

Accession

Primary (citable) accession number: A5N4P4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	July 10, 2007
Last modified:	December 14, 2011
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program