Reviewed, UniProtKB/Swiss-Prot A5N4F7 (SYN_CLOK5)

Names and origin

Protein names

Recommended name:
    Asparaginyl-tRNA synthetase
    EC=6.1.1.22
Alternative name(s):
    Asparagine--tRNA ligase
      Short name=AsnRS

Gene names

Name:	asnS
Ordered Locus Names:	CKL_0118

Organism

Clostridium kluyveri (strain ATCC 8527 / DSM 555 / NCIMB 10680) [Complete proteome] [HAMAP]

Taxonomic identifier

431943 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	463 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + L-asparaginyl-tRNA(Asn). HAMAP MF_00534
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	asparaginyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP aspartyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP asparagine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP aspartate-tRNA ligase activity Inferred from electronic annotation. Source: InterPro nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 463	463	Asparaginyl-tRNA synthetase HAMAP MF_00534		PRO_1000081846

Sequences

	Sequence		Length	Mass (Da)
	A5N4F7-1 [UniParc]. Last modified July 10, 2007. Version 1. Checksum: 5215FCA9681B80B8	FASTA	463	53,227
	10 20 30 40 50 60 MKKTLVKSLY RETEKFIDKD IEISGWIRTI RDSKSFAFIE LNDGSFFKNV QVVLDDKIEN 70 80 90 100 110 120 FKEIIKLPIS SSLSVEGTLI LTPNAKQSFE IKAKKISLEG NSSSEYPLQK KRHTLEYLRT 130 140 150 160 170 180 IAHLRPRSNT FSAVFRVRSI AAYAVHKFFQ EKDFVYVNTP IITGSDCEGA GEMFRITTMD 190 200 210 220 230 240 LNNVPKEEDN SVDFSKDFFG KETNLTVSGQ LSAETMALAF RNVYTFGPTF RAEDSNTSRH 250 260 270 280 290 300 AAEFWMIEPE MAFAELKDYM DTAEELVKYI INYVLEKAPE EMAFFNSFID KSLFDRLNNV 310 320 330 340 350 360 VNSEFGRITY TEAVDILQKS GASFQYPVEW GIDLQTEHER YLTEKVFEKP IFVTDYPKAI 370 380 390 400 410 420 KAFYMRENED GKTVAAADLL VPGVGEIVGG SQREERLSVL EKRISEFGLN KEDYWWYLEL 430 440 450 460 RKYGETKHSG FGLGFERILM YITGMSNIRD VIPFPRTPGS AEF

References

[1]

"The genome of Clostridium kluyveri, a strict anaerobe with unique metabolic features." Seedorf H., Fricke W.F., Veith B., Brueggemann H., Liesegang H., Strittmatter A., Miethke M., Buckel W., Hinderberger J., Li F., Hagemeier C., Thauer R.K., Gottschalk G. Proc. Natl. Acad. Sci. U.S.A. 105:2128-2133(2008) [PubMed: 18218779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000673 Genomic DNA. Translation: EDK32188.1.
RefSeq	YP_001393536.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A5N4F7.
Genome annotation databases
GeneID	5392623.
GenomeReviews	Gene locus CKL_0118 in contig CP000673_GR.
KEGG	ckl:CKL_0118.
NMPDR	fig|431943.4.peg.170.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	LQKKRHS.
Family and domain databases
HAMAP	MF_00534. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II_cons-reg. IPR004522. Asn-tRNA-synth_IIb. IPR002312. Asp-tRNA-synth_IIb. IPR012340. NA-bd_OB-fold. IPR004365. NA_bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit.
Pfam	PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
TIGRFAMs	TIGR00457. asnS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYN_CLOK5

Accession

Primary (citable) accession number: A5N4F7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	July 10, 2007
Last modified:	November 3, 2009
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)