A5N4D3 (PURA_CLOK5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Adenylosuccinate synthetase
Short name=AMPSase
Short name=AdSS
EC=6.3.4.4

Alternative name(s):
IMP--aspartate ligase

Gene names

Name:	purA
Ordered Locus Names:	CKL_0087

Organism

Clostridium kluyveri (strain ATCC 8527 / DSM 555 / NCIMB 10680) [Complete proteome] [HAMAP]

Taxonomic identifier

431943 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	428 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP By similarity. HAMAP MF_00011
Catalytic activity	GTP + IMP + L-aspartate = GDP + phosphate + N(6)-(1,2-dicarboxyethyl)-AMP. HAMAP MF_00011
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_00011
Pathway	Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2. HAMAP MF_00011
Subunit structure	Homodimer By similarity. HAMAP MF_00011
Subcellular location	Cytoplasm By similarity HAMAP MF_00011.
Sequence similarities	Belongs to the adenylosuccinate synthetase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	GTP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	purine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW adenylosuccinate synthase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 428	428	Adenylosuccinate synthetase HAMAP MF_00011		PRO_1000073942
Regions
	Nucleotide binding	12 – 18	7	GTP By similarity
	Nucleotide binding	40 – 42	3	GTP By similarity
	Nucleotide binding	332 – 334	3	GTP By similarity
	Nucleotide binding	414 – 416	3	GTP By similarity
	Region	13 – 16	4	IMP binding By similarity
	Region	38 – 41	4	IMP binding By similarity
	Region	300 – 306	7	Substrate binding By similarity
Sites
	Active site	13	1	Proton acceptor By similarity
	Active site	41	1	Proton donor By similarity
	Metal binding	13	1	Magnesium By similarity
	Metal binding	40	1	Magnesium; via carbonyl oxygen By similarity
	Binding site	130	1	IMP By similarity
	Binding site	144	1	IMP; shared with dimeric partner By similarity
	Binding site	225	1	IMP By similarity
	Binding site	240	1	IMP By similarity
	Binding site	304	1	IMP By similarity
	Binding site	306	1	GTP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5N4D3 [UniParc]. Last modified July 10, 2007. Version 1. Checksum: 156FBA57074AABC6	FASTA	428	47,362
	10 20 30 40 50 60 MSAFIVLGSQ WGDEGKGKMT DYLAKDVDVV VRFQGGNNAG HTVRVGDKEY KLHIIPSGIL 70 80 90 100 110 120 YKDKVNVIGN GVVLDPEALF QEIDYLEKAG VDIQPDNLMI SDRAQLIMPY HKVLDGIKER 130 140 150 160 170 180 SRGKKDIGTT GKGIGPCYTD KMERSGIRVC DLINQDVFKE NLKENLKIKN DIITKVYGEE 190 200 210 220 230 240 ALDFDSICDQ YIEYRKKLAP YVKDISVEVY NSIKNDKKVL FEGAQGTLLD IDYGTYPYVT 250 260 270 280 290 300 SSSTIAGGVC IGAGIGPTLI TGAIGVAKAY TTRVGKGPFP TELFDDTGDW IRKSGREYGV 310 320 330 340 350 360 TTGRARRCGW LDLVILKTSS RVSGLTNFAI TKIDTLGGLE KVKVCTGYKF NGKIIDYVPA 370 380 390 400 410 420 SLQDLAKCEP VYEEFDGWDK DIENAKTYDE LPENAKIYLN KIEEFTNTKI SIVSVGPGRD QTINLNNM

References

[1]

"The genome of Clostridium kluyveri, a strict anaerobe with unique metabolic features." Seedorf H., Fricke W.F., Veith B., Brueggemann H., Liesegang H., Strittmatter A., Miethke M., Buckel W., Hinderberger J., Li F., Hagemeier C., Thauer R.K., Gottschalk G. Proc. Natl. Acad. Sci. U.S.A. 105:2128-2133(2008) [PubMed: 18218779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 8527 / DSM 555 / NCIMB 10680.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000673 Genomic DNA. Translation: EDK32164.1.
RefSeq	YP_001393512.1. NC_009706.1.
3D structure databases
ProteinModelPortal	A5N4D3.
ModBase	Search...
Protein-protein interaction databases
STRING	A5N4D3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5390836.
GenomeReviews	Gene locus CKL_0087 in contig CP000673_GR.
KEGG	ckl:CKL_0087.
NMPDR	fig|431943.4.peg.145.
PATRIC	19461575. VBICloKlu111549_0157.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0104.
HOGENOM	HBG658237.
OMA	DYVQPMK.
ProtClustDB	PRK01117.
Enzyme and pathway databases
BioCyc	CKLU431943:CKL_0087-MONOMER.
Family and domain databases
HAMAP	MF_00011. Adenylosucc_synth. [Tree]
InterPro	IPR018220. Adenylosuccinate_synthase_AS. IPR001114. Adenylosuccinate_synthetase. [Graphical view]
KO	K01939.
PANTHER	PTHR11846. Asucc_synthtase. 1 hit.
Pfam	PF00709. Adenylsucc_synt. 1 hit. [Graphical view]
SMART	SM00788. Adenylsucc_synt. 1 hit. [Graphical view]
TIGRFAMs	TIGR00184. PurA. 1 hit.
PROSITE	PS01266. ADENYLOSUCCIN_SYN_1. 1 hit. PS00513. ADENYLOSUCCIN_SYN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PURA_CLOK5

Accession

Primary (citable) accession number: A5N4D3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	July 10, 2007
Last modified:	December 14, 2011
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program