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A5N3P1

- SYI_CLOK5

UniProt

A5N3P1 - SYI_CLOK5

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Clostridium kluyveri (strain ATCC 8527 / DSM 555 / NCIMB 10680)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 55 (01 Oct 2014)
      Sequence version 1 (10 Jul 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Zinc.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei591 – 5911ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciCKLU431943:GJF1-3745-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:CKL_3752
    OrganismiClostridium kluyveri (strain ATCC 8527 / DSM 555 / NCIMB 10680)
    Taxonomic identifieri431943 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
    ProteomesiUP000002411: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10341034Isoleucine--tRNA ligasePRO_1000088561Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi431943.CKL_3752.

    Structurei

    3D structure databases

    ProteinModelPortaliA5N3P1.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi48 – 5811"HIGH" regionAdd
    BLAST
    Motifi588 – 5925"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246403.
    KOiK01870.
    OMAiRVEHMVE.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02003. Ile_tRNA_synth_type2.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 2 hits.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A5N3P1-1 [UniParc]FASTAAdd to Basket

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    MYKKIDNNKS FVQMEKDILK LWQDRKVVEK SFNSNKDGEY FTFYDGPPTA     50
    NGKPHIGHVL TRVIKDLIPR YKVMKGYKVL RKAGWDTHGL PVELEVEKSL 100
    GISGKPQIEK YGVEDFIKKC KDSVFTYVSQ WRKMSDRIGF WVDMDDPYVT 150
    YDNHYIESEW WALKQIWDKN LLYKGHKIVP YCPRCGTALS SHEVSQGYKD 200
    VKETSVYVKF KIKNEDKYIL AWTTTPWTLP NNMALTINKS YDYVEVINDG 250
    EHLILAEGLL EKLEGEYEVV KKFKGEEMLG IEYEPMFNFT PFEGKAHYVV 300
    HGDYVTLTDG TGIVHTAPAF GEDDSITCIK HNIPMINSVT TQGKFKDEVT 350
    PWKGLFVKDA DPKIIAYLKE KDILYKAEKF THSYPFCWRC DTTLLYYPRD 400
    TWFIRMSAMR DKLIRNTNDT NWYPDNIRTG RFGKFVEGVI DWGLSRERYW 450
    GTPLPIWECE CGHRECIGSI KELREKGINV PDDIELHKPY IDGVKLKCDK 500
    CHKEMERVPE VIDCWFDSGS MPFAQHHYPF ENRELFEKNF PAQFISEAVD 550
    QTRGWFYTLM AISTVLFDKS SFENCLVLGH VLDKHGLKMS KHKGNVLSPE 600
    VVLENEGADA TRWYFYTESA PWLPSRFYEE AVQDSQRKFL GTLWNVYSFY 650
    VLYADLDKFN PLEYSHFVSE NVMDKWVISR LNSLIKITEG NLDNYRITQA 700
    AESIGNFVDE LSNWYVRRNR TRFWNEELAE DKVGAYVTLY NVLNKLCLIA 750
    APFVPFMTEE IYQNLVLSLN KNVPESIHLC KWPEYDLNLV DSDLEKNMEE 800
    CYKIVKLGRS ARNAANIKNR QPLSEMLISV KTLPGYYGDI IKSELNIKNI 850
    VFNADLSKYV NFNIKPNLPV LGKKYGRMIP KIKQSISSMN QMDLAGKINN 900
    NEVVKIKIDE TEIELNSENL LITMEGLEGF AFAGEGSTGI VLETTITEEL 950
    KEEGNLREIL SKIQNMRKES GFEVADKIKL YVSDNEKLEE VVKKFEAQIK 1000
    KETLAVEVAY NENREYSGCN INGEKFNIAV EVLK 1034
    Length:1,034
    Mass (Da):120,017
    Last modified:July 10, 2007 - v1
    Checksum:i2A93120AF7430F78
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000673 Genomic DNA. Translation: EDK35737.1.
    RefSeqiYP_001397108.1. NC_009706.1.

    Genome annotation databases

    EnsemblBacteriaiEDK35737; EDK35737; CKL_3752.
    GeneIDi5391114.
    KEGGickl:CKL_3752.
    PATRICi19469066. VBICloKlu111549_3840.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000673 Genomic DNA. Translation: EDK35737.1 .
    RefSeqi YP_001397108.1. NC_009706.1.

    3D structure databases

    ProteinModelPortali A5N3P1.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 431943.CKL_3752.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai EDK35737 ; EDK35737 ; CKL_3752 .
    GeneIDi 5391114.
    KEGGi ckl:CKL_3752.
    PATRICi 19469066. VBICloKlu111549_3840.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246403.
    KOi K01870.
    OMAi RVEHMVE.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci CKLU431943:GJF1-3745-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02003. Ile_tRNA_synth_type2.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 2 hits.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 8527 / DSM 555 / NCIMB 10680.

    Entry informationi

    Entry nameiSYI_CLOK5
    AccessioniPrimary (citable) accession number: A5N3P1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 20, 2008
    Last sequence update: July 10, 2007
    Last modified: October 1, 2014
    This is version 55 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3