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A5N3P1

- SYI_CLOK5

UniProt

A5N3P1 - SYI_CLOK5

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Clostridium kluyveri (strain ATCC 8527 / DSM 555 / NCIMB 10680)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei591 – 5911ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciCKLU431943:GJF1-3745-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:CKL_3752
OrganismiClostridium kluyveri (strain ATCC 8527 / DSM 555 / NCIMB 10680)
Taxonomic identifieri431943 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
ProteomesiUP000002411: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10341034Isoleucine--tRNA ligasePRO_1000088561Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi431943.CKL_3752.

Structurei

3D structure databases

ProteinModelPortaliA5N3P1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi48 – 5811"HIGH" regionAdd
BLAST
Motifi588 – 5925"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiRVEHMVE.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A5N3P1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MYKKIDNNKS FVQMEKDILK LWQDRKVVEK SFNSNKDGEY FTFYDGPPTA
60 70 80 90 100
NGKPHIGHVL TRVIKDLIPR YKVMKGYKVL RKAGWDTHGL PVELEVEKSL
110 120 130 140 150
GISGKPQIEK YGVEDFIKKC KDSVFTYVSQ WRKMSDRIGF WVDMDDPYVT
160 170 180 190 200
YDNHYIESEW WALKQIWDKN LLYKGHKIVP YCPRCGTALS SHEVSQGYKD
210 220 230 240 250
VKETSVYVKF KIKNEDKYIL AWTTTPWTLP NNMALTINKS YDYVEVINDG
260 270 280 290 300
EHLILAEGLL EKLEGEYEVV KKFKGEEMLG IEYEPMFNFT PFEGKAHYVV
310 320 330 340 350
HGDYVTLTDG TGIVHTAPAF GEDDSITCIK HNIPMINSVT TQGKFKDEVT
360 370 380 390 400
PWKGLFVKDA DPKIIAYLKE KDILYKAEKF THSYPFCWRC DTTLLYYPRD
410 420 430 440 450
TWFIRMSAMR DKLIRNTNDT NWYPDNIRTG RFGKFVEGVI DWGLSRERYW
460 470 480 490 500
GTPLPIWECE CGHRECIGSI KELREKGINV PDDIELHKPY IDGVKLKCDK
510 520 530 540 550
CHKEMERVPE VIDCWFDSGS MPFAQHHYPF ENRELFEKNF PAQFISEAVD
560 570 580 590 600
QTRGWFYTLM AISTVLFDKS SFENCLVLGH VLDKHGLKMS KHKGNVLSPE
610 620 630 640 650
VVLENEGADA TRWYFYTESA PWLPSRFYEE AVQDSQRKFL GTLWNVYSFY
660 670 680 690 700
VLYADLDKFN PLEYSHFVSE NVMDKWVISR LNSLIKITEG NLDNYRITQA
710 720 730 740 750
AESIGNFVDE LSNWYVRRNR TRFWNEELAE DKVGAYVTLY NVLNKLCLIA
760 770 780 790 800
APFVPFMTEE IYQNLVLSLN KNVPESIHLC KWPEYDLNLV DSDLEKNMEE
810 820 830 840 850
CYKIVKLGRS ARNAANIKNR QPLSEMLISV KTLPGYYGDI IKSELNIKNI
860 870 880 890 900
VFNADLSKYV NFNIKPNLPV LGKKYGRMIP KIKQSISSMN QMDLAGKINN
910 920 930 940 950
NEVVKIKIDE TEIELNSENL LITMEGLEGF AFAGEGSTGI VLETTITEEL
960 970 980 990 1000
KEEGNLREIL SKIQNMRKES GFEVADKIKL YVSDNEKLEE VVKKFEAQIK
1010 1020 1030
KETLAVEVAY NENREYSGCN INGEKFNIAV EVLK
Length:1,034
Mass (Da):120,017
Last modified:July 10, 2007 - v1
Checksum:i2A93120AF7430F78
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000673 Genomic DNA. Translation: EDK35737.1.
RefSeqiYP_001397108.1. NC_009706.1.

Genome annotation databases

EnsemblBacteriaiEDK35737; EDK35737; CKL_3752.
GeneIDi5391114.
KEGGickl:CKL_3752.
PATRICi19469066. VBICloKlu111549_3840.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000673 Genomic DNA. Translation: EDK35737.1 .
RefSeqi YP_001397108.1. NC_009706.1.

3D structure databases

ProteinModelPortali A5N3P1.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 431943.CKL_3752.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai EDK35737 ; EDK35737 ; CKL_3752 .
GeneIDi 5391114.
KEGGi ckl:CKL_3752.
PATRICi 19469066. VBICloKlu111549_3840.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi RVEHMVE.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci CKLU431943:GJF1-3745-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 8527 / DSM 555 / NCIMB 10680.

Entry informationi

Entry nameiSYI_CLOK5
AccessioniPrimary (citable) accession number: A5N3P1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: July 10, 2007
Last modified: October 29, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3