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A5N399 (SYL_CLOK5) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:CKL_3605
OrganismClostridium kluyveri (strain ATCC 8527 / DSM 555 / NCIMB 10680) [Complete proteome] [HAMAP]
Taxonomic identifier431943 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length811 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 811811Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000334745

Regions

Motif38 – 4912"HIGH" region HAMAP-Rule MF_00049
Motif570 – 5745"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5731ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A5N399 [UniParc].

Last modified July 10, 2007. Version 1.
Checksum: 4F9A8EF4DF120AC5

FASTA81193,287
        10         20         30         40         50         60 
MYSTKTDKKW QQKWDQTNIY TFDEKKSSKK LYTLEMFSYP SGSNLHAGHW FNYAPVDSWA 

        70         80         90        100        110        120 
RMKRMQGYNV FQPMGFDAFG LPAENYAIKT GIHPKDSTEK NIDTMEKQLK SMGAMFNWEN 

       130        140        150        160        170        180 
EVVTCRPDYY KWNQWLFLQL YKHDLAYRKN APVNWCPSCN TVLANEQVKE GECERCGTEV 

       190        200        210        220        230        240 
TKKDLTQWFF KITDYTEELL KDLDNIDWPE KTKAMQRHWM GKSTGTDVTF KVADSDIKFN 

       250        260        270        280        290        300 
VFTTRVDTLF GVTYVVISPE NDLVDSVTKE EYKEEIEKYR EAAKKQTDIE RQSITREKTG 

       310        320        330        340        350        360 
VSTGSYAINP INGRKVPIWI GDYVLNTYGT GAVMAVPAHD ERDFEFATKY KLPIERVIEG 

       370        380        390        400        410        420 
GSSLPYVEYG KMINSDKFNG LYTPEGKEAV TKELENIGLG SGKTNYRLRD WLVSRQRYWG 

       430        440        450        460        470        480 
TPIPIIYCKK CGTVPVPESD LPVELPYNVQ FSPDGKSPLL KSEEFMNTTC PICGEPAKRE 

       490        500        510        520        530        540 
ADTLDTFVCS SWYYLRYADN KNTEKAFDKD KINKLLPVDM YVGGPEHACM HLLYARFITK 

       550        560        570        580        590        600 
ALRDMGFLNF DEPFLSLRHQ GLILGPDGQK MSKSKGNTIS PDDCIQKYGS DVFRMYLMFG 

       610        620        630        640        650        660 
FDYAEGGAWN DDGIKSMSKF VDRIERIVGN IKELINSGKN FKNSMDAAEK ELNYSRNYSI 

       670        680        690        700        710        720 
KSVSEDAGKF QFNTAIARIM EFTNSLYKYI QEDTKNISLL KDTILDFIKI IAPFAPHFAE 

       730        740        750        760        770        780 
EQWEVLGQKY SIFNEKWPEF DPKALVKEEV EIAIQINGKI KAKINIPTNL SDEQIKELSI 

       790        800        810 
SNDNIKPLLE GKNIKKVIVV KGRLVNIVVK P 

« Hide

References

[1]"The genome of Clostridium kluyveri, a strict anaerobe with unique metabolic features."
Seedorf H., Fricke W.F., Veith B., Brueggemann H., Liesegang H., Strittmatter A., Miethke M., Buckel W., Hinderberger J., Li F., Hagemeier C., Thauer R.K., Gottschalk G.
Proc. Natl. Acad. Sci. U.S.A. 105:2128-2133(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 8527 / DSM 555 / NCIMB 10680.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000673 Genomic DNA. Translation: EDK35595.1.
RefSeqYP_001396966.1. NC_009706.1.

3D structure databases

ProteinModelPortalA5N399.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING431943.CKL_3605.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEDK35595; EDK35595; CKL_3605.
GeneID5394150.
KEGGckl:CKL_3605.
PATRIC19468770. VBICloKlu111549_3697.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycCKLU431943:GJF1-3598-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
ProtoNetSearch...

Entry information

Entry nameSYL_CLOK5
AccessionPrimary (citable) accession number: A5N399
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: July 10, 2007
Last modified: April 16, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries