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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Clostridium kluyveri (strain ATCC 8527 / DSM 555 / NCIMB 10680)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei573 – 5731ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCKLU431943:GJF1-3598-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:CKL_3605
OrganismiClostridium kluyveri (strain ATCC 8527 / DSM 555 / NCIMB 10680)
Taxonomic identifieri431943 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
ProteomesiUP000002411 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 811811Leucine--tRNA ligasePRO_0000334745Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi431943.CKL_3605.

Structurei

3D structure databases

ProteinModelPortaliA5N399.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi38 – 4912"HIGH" regionAdd
BLAST
Motifi570 – 5745"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.

Sequencei

Sequence statusi: Complete.

A5N399-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYSTKTDKKW QQKWDQTNIY TFDEKKSSKK LYTLEMFSYP SGSNLHAGHW
60 70 80 90 100
FNYAPVDSWA RMKRMQGYNV FQPMGFDAFG LPAENYAIKT GIHPKDSTEK
110 120 130 140 150
NIDTMEKQLK SMGAMFNWEN EVVTCRPDYY KWNQWLFLQL YKHDLAYRKN
160 170 180 190 200
APVNWCPSCN TVLANEQVKE GECERCGTEV TKKDLTQWFF KITDYTEELL
210 220 230 240 250
KDLDNIDWPE KTKAMQRHWM GKSTGTDVTF KVADSDIKFN VFTTRVDTLF
260 270 280 290 300
GVTYVVISPE NDLVDSVTKE EYKEEIEKYR EAAKKQTDIE RQSITREKTG
310 320 330 340 350
VSTGSYAINP INGRKVPIWI GDYVLNTYGT GAVMAVPAHD ERDFEFATKY
360 370 380 390 400
KLPIERVIEG GSSLPYVEYG KMINSDKFNG LYTPEGKEAV TKELENIGLG
410 420 430 440 450
SGKTNYRLRD WLVSRQRYWG TPIPIIYCKK CGTVPVPESD LPVELPYNVQ
460 470 480 490 500
FSPDGKSPLL KSEEFMNTTC PICGEPAKRE ADTLDTFVCS SWYYLRYADN
510 520 530 540 550
KNTEKAFDKD KINKLLPVDM YVGGPEHACM HLLYARFITK ALRDMGFLNF
560 570 580 590 600
DEPFLSLRHQ GLILGPDGQK MSKSKGNTIS PDDCIQKYGS DVFRMYLMFG
610 620 630 640 650
FDYAEGGAWN DDGIKSMSKF VDRIERIVGN IKELINSGKN FKNSMDAAEK
660 670 680 690 700
ELNYSRNYSI KSVSEDAGKF QFNTAIARIM EFTNSLYKYI QEDTKNISLL
710 720 730 740 750
KDTILDFIKI IAPFAPHFAE EQWEVLGQKY SIFNEKWPEF DPKALVKEEV
760 770 780 790 800
EIAIQINGKI KAKINIPTNL SDEQIKELSI SNDNIKPLLE GKNIKKVIVV
810
KGRLVNIVVK P
Length:811
Mass (Da):93,287
Last modified:July 10, 2007 - v1
Checksum:i4F9A8EF4DF120AC5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000673 Genomic DNA. Translation: EDK35595.1.
RefSeqiWP_012103927.1. NC_009706.1.
YP_001396966.1. NC_009706.1.

Genome annotation databases

EnsemblBacteriaiEDK35595; EDK35595; CKL_3605.
KEGGickl:CKL_3605.
PATRICi19468770. VBICloKlu111549_3697.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000673 Genomic DNA. Translation: EDK35595.1.
RefSeqiWP_012103927.1. NC_009706.1.
YP_001396966.1. NC_009706.1.

3D structure databases

ProteinModelPortaliA5N399.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi431943.CKL_3605.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiEDK35595; EDK35595; CKL_3605.
KEGGickl:CKL_3605.
PATRICi19468770. VBICloKlu111549_3697.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciCKLU431943:GJF1-3598-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 8527 / DSM 555 / NCIMB 10680.

Entry informationi

Entry nameiSYL_CLOK5
AccessioniPrimary (citable) accession number: A5N399
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: July 10, 2007
Last modified: May 27, 2015
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.