A5N379 (ACPS_CLOK5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Holo-[acyl-carrier-protein] synthase
Short name=Holo-ACP synthase
EC=2.7.8.7

Alternative name(s):
4'-phosphopantetheinyl transferase AcpS

Gene names

Name:	acpS
Ordered Locus Names:	CKL_3584

Organism

Clostridium kluyveri (strain ATCC 8527 / DSM 555 / NCIMB 10680) [Complete proteome] [HAMAP]

Taxonomic identifier

431943 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	134 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Transfers the 4'-phosphopantetheine moiety from coenzyme A to a Ser of acyl-carrier-protein By similarity. HAMAP MF_00101
Catalytic activity	CoA-(4'-phosphopantetheine) + apo-[acyl-carrier-protein] = adenosine 3',5'-bisphosphate + holo-[acyl-carrier-protein]. HAMAP MF_00101
Cofactor	Magnesium By similarity. HAMAP MF_00101
Subcellular location	Cytoplasm By similarity HAMAP MF_00101.
Sequence similarities	Belongs to the P-Pant transferase superfamily. AcpS family.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Lipid synthesis
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	fatty acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW macromolecule biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	holo-[acyl-carrier-protein] synthase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 134	134	Holo-[acyl-carrier-protein] synthase HAMAP MF_00101		PRO_1000075636
Sites
	Metal binding	8	1	Magnesium By similarity
	Metal binding	56	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5N379 [UniParc]. Last modified July 10, 2007. Version 1. Checksum: 8680191231FC569C	FASTA	134	15,137
	10 20 30 40 50 60 MIFGVGVDIV EIRRIKEAIE KHNTFIDRIF SKNEIEYLKN RNLRPEFVAG RFAAKEAVVK 70 80 90 100 110 120 SLGSGFRGFD FKDIEIDRTA SGRPTVVLKG KAKLMANKYG NYKIHLSISH GVDNAIAYAI 130 MEVDKIEDSD CKDI

References

[1]

"The genome of Clostridium kluyveri, a strict anaerobe with unique metabolic features." Seedorf H., Fricke W.F., Veith B., Brueggemann H., Liesegang H., Strittmatter A., Miethke M., Buckel W., Hinderberger J., Li F., Hagemeier C., Thauer R.K., Gottschalk G. Proc. Natl. Acad. Sci. U.S.A. 105:2128-2133(2008) [PubMed: 18218779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 8527 / DSM 555 / NCIMB 10680.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000673 Genomic DNA. Translation: EDK35575.1.
RefSeq	YP_001396946.1. NC_009706.1.
3D structure databases
ProteinModelPortal	A5N379.
ModBase	Search...
Protein-protein interaction databases
STRING	A5N379.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5392104.
GenomeReviews	Gene locus CKL_3584 in contig CP000673_GR.
KEGG	ckl:CKL_3584.
NMPDR	fig|431943.4.peg.3593.
PATRIC	19468728. VBICloKlu111549_3677.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0736.
HOGENOM	HBG734479.
OMA	NSSAIAY.
ProtClustDB	PRK00070.
Enzyme and pathway databases
BioCyc	CKLU431943:CKL_3584-MONOMER.
Family and domain databases
HAMAP	MF_00101. AcpS. [Tree]
InterPro	IPR008278. 4-PPantetheinyl_Trfase. IPR002582. ACPS. IPR004568. PPantethiene-prot_Trfase. [Graphical view]
Gene3D	G3DSA:3.90.470.20. G3DSA:3.90.470.20. 1 hit.
KO	K00997.
Pfam	PF01648. ACPS. 1 hit. [Graphical view]
ProDom	PD004282. PPantethiene-prot_Trfase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SUPFAM	SSF56214. 4-PPT_transf. 1 hit.
TIGRFAMs	TIGR00516. AcpS. 1 hit. TIGR00556. Pantethn_trn. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ACPS_CLOK5

Accession

Primary (citable) accession number: A5N379

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	July 10, 2007
Last modified:	December 14, 2011
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program