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A5N2N8 (PGK_CLOK5) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 46. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoglycerate kinase

EC=2.7.2.3
Gene names
Name:pgk
Ordered Locus Names:CKL_3381
OrganismClostridium kluyveri (strain ATCC 8527 / DSM 555 / NCIMB 10680) [Complete proteome] [HAMAP]
Taxonomic identifier431943 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length398 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate. HAMAP-Rule MF_00145

Pathway

Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5. HAMAP-Rule MF_00145

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00145

Subcellular location

Cytoplasm Potential HAMAP-Rule MF_00145.

Sequence similarities

Belongs to the phosphoglycerate kinase family.

Ontologies

Keywords
   Biological processGlycolysis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processglycolysis

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

phosphoglycerate kinase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 398398Phosphoglycerate kinase HAMAP-Rule MF_00145
PRO_1000076585

Regions

Nucleotide binding354 – 3574ATP By similarity
Region23 – 253Substrate binding By similarity
Region61 – 644Substrate binding By similarity

Sites

Binding site381Substrate By similarity
Binding site1221Substrate By similarity
Binding site1551Substrate By similarity
Binding site2061ATP By similarity
Binding site2971ATP; via carbonyl oxygen By similarity
Binding site3281ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A5N2N8 [UniParc].

Last modified July 10, 2007. Version 1.
Checksum: 5D91250193455F39

FASTA39843,173
        10         20         30         40         50         60 
MVFNKKTIED VDVKGKRVLV RCDFNVPLQE GKITDENRLI GSLPTIKYLM ENNAKVILCS 

        70         80         90        100        110        120 
HLGKPKGEVK PEMSLLPVAK RLSELLKKEV VFAADDNVVG ENAKAAVKNM KDGDVILLQN 

       130        140        150        160        170        180 
TRYRIEETKN QDNFSKELAS LGEIFVNDAF GTAHRAHCST VGVTKFLPTA VCGYLIQKEL 

       190        200        210        220        230        240 
EFLGNAIENP SRPFTAILGG VKVSDKINVI NNLLEKVDTL IIGGGMSYTF ARAQGYTIGT 

       250        260        270        280        290        300 
SVVEEDKIEY AKEMIDKAKE KGIKLLLPID RVVTDKFDES AEPILEDDKN IKDGYMGMDI 

       310        320        330        340        350        360 
GPKTAKVYAD AIKDSKTIIW NGPMGVFEFK NFAKGTFAVA KAMAESGAIT IIGGGDSAAA 

       370        380        390 
INQLGFGDKM THISTGGGAS LEFLGGEELP GISALNNK 

« Hide

References

[1]"The genome of Clostridium kluyveri, a strict anaerobe with unique metabolic features."
Seedorf H., Fricke W.F., Veith B., Brueggemann H., Liesegang H., Strittmatter A., Miethke M., Buckel W., Hinderberger J., Li F., Hagemeier C., Thauer R.K., Gottschalk G.
Proc. Natl. Acad. Sci. U.S.A. 105:2128-2133(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 8527 / DSM 555 / NCIMB 10680.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000673 Genomic DNA. Translation: EDK35384.1.
RefSeqYP_001396755.1. NC_009706.1.

3D structure databases

ProteinModelPortalA5N2N8.
SMRA5N2N8. Positions 3-398.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING431943.CKL_3381.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEDK35384; EDK35384; CKL_3381.
GeneID5391787.
KEGGckl:CKL_3381.
PATRIC19468320. VBICloKlu111549_3485.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0126.
HOGENOMHOG000227108.
KOK00927.
OMADMIFDIG.
OrthoDBEOG64N9Z0.
ProtClustDBPRK00073.

Enzyme and pathway databases

BioCycCKLU431943:GJF1-3374-MONOMER.
UniPathwayUPA00109; UER00185.

Family and domain databases

Gene3D3.40.50.1260. 1 hit.
3.40.50.1270. 1 hit.
HAMAPMF_00145. Phosphoglyc_kinase.
InterProIPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view]
PANTHERPTHR11406. PTHR11406. 1 hit.
PfamPF00162. PGK. 1 hit.
[Graphical view]
PIRSFPIRSF000724. Pgk. 1 hit.
PRINTSPR00477. PHGLYCKINASE.
SUPFAMSSF53748. SSF53748. 1 hit.
PROSITEPS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePGK_CLOK5
AccessionPrimary (citable) accession number: A5N2N8
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: July 10, 2007
Last modified: February 19, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways