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A5N1D3 (THIM_CLOK5) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 51. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Hydroxyethylthiazole kinase

EC=2.7.1.50
Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinase
Short name=TH kinase
Short name=Thz kinase
Gene names
Name:thiM
Ordered Locus Names:CKL_2918
OrganismClostridium kluyveri (strain ATCC 8527 / DSM 555 / NCIMB 10680) [Complete proteome] [HAMAP]
Taxonomic identifier431943 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length269 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole. HAMAP-Rule MF_00228

Cofactor

Magnesium By similarity. HAMAP-Rule MF_00228

Pathway

Cofactor biosynthesis; thiamine diphosphate biosynthesis; 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole: step 1/1. HAMAP-Rule MF_00228

Sequence similarities

Belongs to the Thz kinase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 269269Hydroxyethylthiazole kinase HAMAP-Rule MF_00228
PRO_0000336551

Sites

Binding site481Substrate; via amide nitrogen By similarity
Binding site1241ATP By similarity
Binding site1701ATP By similarity
Binding site1971Substrate; via amide nitrogen By similarity

Sequences

Sequence LengthMass (Da)Tools
A5N1D3 [UniParc].

Last modified July 10, 2007. Version 1.
Checksum: 27ABE775AC3BC4AA

FASTA26928,946
        10         20         30         40         50         60 
MNDLKLDKVS KVLKEVRKSV PLVHCITNYV TINDCANILL SYGASPAMCE AYDEVFDFVK 

        70         80         90        100        110        120 
ISSALYINIG TFTREQESSA ILAAVSAKTH NIPVVLDPVA CAAIPKKIQF IDKLFKVGRV 

       130        140        150        160        170        180 
DVIKGNIGEI KFLAGETSNV KGVDSLEDGE GALECSRILS EKYNCVVAAT GKKDFIVQGK 

       190        200        210        220        230        240 
NSAIIENGTE MLTNITGAGC MLGALCAAAC GAFEDKFMAV VGAILSINIA SEEAYKEAKA 

       250        260 
PGSFRVKLID CIYELSEEKL KKEGKIAWN 

« Hide

References

[1]"The genome of Clostridium kluyveri, a strict anaerobe with unique metabolic features."
Seedorf H., Fricke W.F., Veith B., Brueggemann H., Liesegang H., Strittmatter A., Miethke M., Buckel W., Hinderberger J., Li F., Hagemeier C., Thauer R.K., Gottschalk G.
Proc. Natl. Acad. Sci. U.S.A. 105:2128-2133(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 8527 / DSM 555 / NCIMB 10680.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000673 Genomic DNA. Translation: EDK34929.1.
RefSeqYP_001396300.1. NC_009706.1.

3D structure databases

ProteinModelPortalA5N1D3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING431943.CKL_2918.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEDK34929; EDK34929; CKL_2918.
GeneID5391070.
KEGGckl:CKL_2918.
PATRIC19467385. VBICloKlu111549_3026.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2145.
HOGENOMHOG000114352.
KOK00878.
OMANACTQLA.
OrthoDBEOG628F8M.

Enzyme and pathway databases

BioCycCKLU431943:GJF1-2911-MONOMER.
UniPathwayUPA00060; UER00139.

Family and domain databases

Gene3D3.40.1190.20. 1 hit.
HAMAPMF_00228. Thz_kinase.
InterProIPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
[Graphical view]
PfamPF02110. HK. 1 hit.
[Graphical view]
PIRSFPIRSF000513. Thz_kinase. 1 hit.
PRINTSPR01099. HYETHTZKNASE.
SUPFAMSSF53613. SSF53613. 1 hit.
TIGRFAMsTIGR00694. thiM. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTHIM_CLOK5
AccessionPrimary (citable) accession number: A5N1D3
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: July 10, 2007
Last modified: June 11, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways