A5N0Q2 (PUR5_CLOK5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine cyclo-ligase
EC=6.3.3.1

Alternative name(s):
AIR synthase
AIRS
Phosphoribosyl-aminoimidazole synthetase

Gene names

Name:	purM
Ordered Locus Names:	CKL_2686

Organism

Clostridium kluyveri (strain ATCC 8527 / DSM 555 / NCIMB 10680) [Complete proteome] [HAMAP]

Taxonomic identifier

431943 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium › Clostridium kluyveri

Protein attributes

Sequence length	331 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole. HAMAP-Rule MF_00741
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/2. HAMAP-Rule MF_00741
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the AIR synthase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine cyclo-ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 331	331	Phosphoribosylformylglycinamidine cyclo-ligase HAMAP-Rule MF_00741		PRO_1000083454

Sequences

	Sequence		Length	Mass (Da)
	A5N0Q2 [UniParc]. Last modified July 10, 2007. Version 1. Checksum: 0E04A618EE4C7579	FASTA	331	36,276
	10 20 30 40 50 60 MVTYKDSGVN IEEGYKSVKL MKEYSAQTFI PGVLNGLGSF AGMFELGKYK NPVLVSGTDG 70 80 90 100 110 120 VGTKLKIAFE MKIYDTVGID CVAMCVNDIL CHGAKPLFFL DYLACSNLEA EVAAELVKGI 130 140 150 160 170 180 SKGCMDAGCA LIGGETAEMP GFYSKGEYDM AGFAVGVVEK DNIINGSTVE EGDVLVGIAS 190 200 210 220 230 240 SGVHSNGYSL VRKLVDNFQV DFFGSALGEV LLTPTRIYVK PVLKLLEKFK IKAMAHITGG 250 260 270 280 290 300 GFYENIPRMF KEDFTAVIDK NSFEMPEIFK YIMDLGVDEE HMYNTYNMGI GFVLCVDSKD 310 320 330 APDIIKDLNE MGEKAYIIGH VKRREKRVCL K

References

[1]

"The genome of Clostridium kluyveri, a strict anaerobe with unique metabolic features." Seedorf H., Fricke W.F., Veith B., Brueggemann H., Liesegang H., Strittmatter A., Miethke M., Buckel W., Hinderberger J., Li F., Hagemeier C., Thauer R.K., Gottschalk G. Proc. Natl. Acad. Sci. U.S.A. 105:2128-2133(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 8527 / DSM 555 / NCIMB 10680.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000673 Genomic DNA. Translation: EDK34698.1.
RefSeq	YP_001396069.1. NC_009706.1.
3D structure databases
ProteinModelPortal	A5N0Q2.
SMR	A5N0Q2. Positions 16-328.
ModBase	Search...
Protein-protein interaction databases
STRING	431943.CKL_2686.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EDK34698; EDK34698; CKL_2686.
GeneID	5390661.
KEGG	ckl:CKL_2686.
PATRIC	19466911. VBICloKlu111549_2790.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0150.
HOGENOM	HOG000229091.
KO	K01933.
OMA	IDMIAMN.
ProtClustDB	PRK05385.
Enzyme and pathway databases
BioCyc	CKLU431943:GJF1-2679-MONOMER.
UniPathway	UPA00074; UER00129.
Family and domain databases
HAMAP	MF_00741_B. AIRS_B.
InterPro	IPR010918. AIR_synth_C_dom. IPR000728. AIR_synth_N_dom. IPR004733. PurM_cligase. IPR016188. PurM_N-like. [Graphical view]
Pfam	PF00586. AIRS. 1 hit. PF02769. AIRS_C. 1 hit. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 1 hit. SSF55326. PurM_N-like. 1 hit.
TIGRFAMs	TIGR00878. purM. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PUR5_CLOK5

Accession

Primary (citable) accession number: A5N0Q2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	July 10, 2007
Last modified:	May 1, 2013
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program