A5N0Q0 (PUR9_CLOK5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Bifunctional purine biosynthesis protein PurH

Including the following 2 domains:

1. Phosphoribosylaminoimidazolecarboxamide formyltransferase
   EC=2.1.2.3
   Alternative name(s):
   AICAR transformylase
2. IMP cyclohydrolase
   EC=3.5.4.10
   Alternative name(s):
   ATIC
   IMP synthase
   Inosinicase

Gene names

Name:	purH
Ordered Locus Names:	CKL_2684

Organism

Clostridium kluyveri (strain ATCC 8527 / DSM 555 / NCIMB 10680) [Complete proteome] [HAMAP]

Taxonomic identifier

431943 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium › Clostridium kluyveri

Protein attributes

Sequence length	499 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide. HAMAP-Rule MF_00139 IMP + H2O = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide. HAMAP-Rule MF_00139
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (10-formyl THF route): step 1/1. HAMAP-Rule MF_00139 Purine metabolism; IMP biosynthesis via de novo pathway; IMP from 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide: step 1/1.
Domain	The IMP cyclohydrolase activity resides in the N-terminal region By similarity. HAMAP-Rule MF_00139
Sequence similarities	Belongs to the PurH family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Molecular function	Hydrolase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological_process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	IMP cyclohydrolase activity Inferred from electronic annotation. Source: HAMAP phosphoribosylaminoimidazolecarboxamide formyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 499	499	Bifunctional purine biosynthesis protein PurH HAMAP-Rule MF_00139		PRO_1000076479

Sequences

	Sequence		Length	Mass (Da)
	A5N0Q0 [UniParc]. Last modified July 10, 2007. Version 1. Checksum: C01A33D8D18AD82A	FASTA	499	55,653
	10 20 30 40 50 60 MINRALISVY NKEGLLELAQ FLKNKGVELI STGGTYKYLE QNDIQVTEVS KITGFDEILD 70 80 90 100 110 120 GRVKTLHPVI HSGILAKRDN KEHMDTIAKK DILPIDMVVV NLYPFFDKVD DNITFEEKVE 130 140 150 160 170 180 FIDIGGPTMI RAAAKNFKDV IVVTDVGDYS KLIEEIDSKG DVPYDFRKKL AGKVFNLMSA 190 200 210 220 230 240 YDGAISNFLL EEDYPEYLSL SYKKMDNLRY GENPHQSAAY YTATAGTAPM KDFTQLNGKQ 250 260 270 280 290 300 LSYNNIKDMD IAWKVVNEFE EICCVAVKHN TPCGVALGKD LYEAYVKTYE CDPTSIFGGI 310 320 330 340 350 360 IAVNRKLDVK TAEEISKIFV EIVIAPDFDE EALKVLMEKK NLRIIKCSVK PTNSMEIAKV 370 380 390 400 410 420 DGGILVQSAD DKLVENMEVV TDKKPSKEEI NNLIFGMKVC KYVKSNAIVV VKDFMAKGIG 430 440 450 460 470 480 GGQVNRIWPT CHALDRAGDG VVLASDAFFP FNDVVCEAAK YGIKAIIQPG GSVRDKDSIE 490 ECNKNGISMV FTGVRHFKH

References

[1]

"The genome of Clostridium kluyveri, a strict anaerobe with unique metabolic features." Seedorf H., Fricke W.F., Veith B., Brueggemann H., Liesegang H., Strittmatter A., Miethke M., Buckel W., Hinderberger J., Li F., Hagemeier C., Thauer R.K., Gottschalk G. Proc. Natl. Acad. Sci. U.S.A. 105:2128-2133(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 8527 / DSM 555 / NCIMB 10680.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000673 Genomic DNA. Translation: EDK34696.1.
RefSeq	YP_001396067.1. NC_009706.1.
3D structure databases
ProteinModelPortal	A5N0Q0.
ModBase	Search...
Protein-protein interaction databases
STRING	431943.CKL_2684.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EDK34696; EDK34696; CKL_2684.
GeneID	5390659.
KEGG	ckl:CKL_2684.
PATRIC	19466907. VBICloKlu111549_2788.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0138.
HOGENOM	HOG000230373.
KO	K00602.
OMA	DLLFAWK.
ProtClustDB	PRK00881.
Enzyme and pathway databases
BioCyc	CKLU431943:GJF1-2677-MONOMER.
UniPathway	UPA00074; UER00133. UPA00074; UER00135.
Family and domain databases
Gene3D	3.40.140.20. 2 hits. 3.40.50.1380. 1 hit.
HAMAP	MF_00139. PurH.
InterPro	IPR024051. AICAR_Tfase_dom. IPR002695. AICARFT_IMPCHas. IPR016193. Cytidine_deaminase-like. IPR011607. MGS-like_dom. [Graphical view]
PANTHER	PTHR11692. PTHR11692. 1 hit.
Pfam	PF01808. AICARFT_IMPCHas. 1 hit. PF02142. MGS. 1 hit. [Graphical view]
PIRSF	PIRSF000414. AICARFT_IMPCHas. 1 hit.
SMART	SM00798. AICARFT_IMPCHas. 1 hit. SM00851. MGS. 1 hit. [Graphical view]
SUPFAM	SSF53927. Cytidine_deaminase-like. 1 hit. SSF52335. MGS-like_dom. 1 hit.
TIGRFAMs	TIGR00355. purH. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PUR9_CLOK5

Accession

Primary (citable) accession number: A5N0Q0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	July 10, 2007
Last modified:	May 1, 2013
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program