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Protein

Lipoyl synthase, apicoplast

Gene

lipA

Organism
Plasmodium vivax (strain Salvador I)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.

Catalytic activityi

Protein N6-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + 2 reduced [2Fe-2S] ferredoxin = protein N6-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine + 2 oxidized [2Fe-2S] ferredoxin.

Cofactori

[4Fe-4S] clusterNote: Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.

Pathwayi: protein lipoylation via endogenous pathway

This protein is involved in step 2 of the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein].
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Lipoyl synthase, apicoplast (lipA)
This subpathway is part of the pathway protein lipoylation via endogenous pathway, which is itself part of Protein modification.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein], the pathway protein lipoylation via endogenous pathway and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi177Iron-sulfur 1 (4Fe-4S)1
Metal bindingi182Iron-sulfur 1 (4Fe-4S)1
Metal bindingi188Iron-sulfur 1 (4Fe-4S)1
Metal bindingi203Iron-sulfur 2 (4Fe-4S-S-AdoMet)1
Metal bindingi207Iron-sulfur 2 (4Fe-4S-S-AdoMet)1
Metal bindingi210Iron-sulfur 2 (4Fe-4S-S-AdoMet)1

GO - Molecular functioni

Keywordsi

Molecular functionTransferase
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase, apicoplast (EC:2.8.1.8)
Alternative name(s):
Lipoate synthase
Short name:
LS
Short name:
Lip-syn
Lipoic acid synthase
Gene namesi
Name:lipA
ORF Names:PVX_083125
OrganismiPlasmodium vivax (strain Salvador I)
Taxonomic identifieri126793 [NCBI]
Taxonomic lineageiEukaryotaAlveolataApicomplexaAconoidasidaHaemosporidaPlasmodiidaePlasmodiumPlasmodium (Plasmodium)
Proteomesi
  • UP000008333 Componentsi: Chromosome 12, Unassembled WGS sequence

Organism-specific databases

EuPathDBiPlasmoDB:PVX_083125.

Subcellular locationi

  • apicoplast

GO - Cellular componenti

Keywords - Cellular componenti

Apicoplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 25Add BLAST25
ChainiPRO_000039823226 – 442Lipoyl synthase, apicoplastAdd BLAST417

Interactioni

Protein-protein interaction databases

STRINGi5855.PVX_083125.

Structurei

3D structure databases

ProteinModelPortaliA5K883.
SMRiA5K883.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Lipoyl synthase family.

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000235998.
InParanoidiA5K883.
KOiK03644.
OMAiGRCPNRG.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth. 1 hit.
InterProiView protein in InterPro
IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR031691. LIAS_N.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
PfamiView protein in Pfam
PF16881. LIAS_N. 1 hit.
PF04055. Radical_SAM. 1 hit.
SFLDiSFLDG01058. lipoyl_synthase_like. 1 hit.
SFLDS00029. Radical_SAM. 1 hit.
SMARTiView protein in SMART
SM00729. Elp3. 1 hit.
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A5K883-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHVLTPSLYI YAFFIVCVRL KCGRSKRVAN AKHATYDMPP KGLRVRDMLE
60 70 80 90 100
KTAQQNCNQR KRGKCRKFFF LWKMDKMGDT HLGGQANGRK NLLRSESATD
110 120 130 140 150
EASLGGNPLK EKLKESPANW GKDKQEEQQS TDRLPLPKVG NKMPEKKPDW
160 170 180 190 200
FHVPAPTGKK YNELKADLKK LKLHTVCEEA QCPNIGECWN IGTATIMLLG
210 220 230 240 250
DTCTRGCKFC SIKTSSKPLP PDANEPFNTA KAICEWDINY VVLTSVDRDD
260 270 280 290 300
LPDGGASHFA KTIELIKFSR PEILIECLVS DFQGNVDSIR KLANSGMEVY
310 320 330 340 350
AHNIETVRRL QKFVRDRRAN YEQSLWVLKT AKEINPLLYT KTSIMLGLGE
360 370 380 390 400
TKQEVLQAMA DVRQNNIDVI TFGQYLRPTK NHLNVVEYVS PQMFDYYKEE
410 420 430 440
GMKMGFKYIA SGPLVRSSYK AGEYFMKNLV EQRRGVKLHA EG
Length:442
Mass (Da):50,317
Last modified:July 10, 2007 - v1
Checksum:i0EFA968BB12F4E3F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAKM01000009 Genomic DNA. Translation: EDL44497.1.
RefSeqiXP_001614224.1. XM_001614174.1.

Genome annotation databases

EnsemblProtistsiEDL44497; EDL44497; PVX_083125.
GeneIDi5473509.
KEGGipvx:PVX_083125.

Similar proteinsi

Entry informationi

Entry nameiLIPA_PLAVS
AccessioniPrimary (citable) accession number: A5K883
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: July 10, 2007
Last modified: October 25, 2017
This is version 63 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families