Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

A5JTQ3

- XYL2_MEDSV

UniProt

A5JTQ3 - XYL2_MEDSV

Protein

Beta-xylosidase/alpha-L-arabinofuranosidase 2

Gene

Xyl2

Organism
Medicago sativa subsp. varia (Alfalfa) (Medicago varia)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 27 (01 Oct 2014)
      Sequence version 1 (26 Jun 2007)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    A bifunctional beta-xylosidase/alpha-L-arabinosidase, exo-enzyme that acts synergistically with endohydrolases. Releases xylose and arabinose from cell walls By similarity.By similarity

    Catalytic activityi

    Hydrolysis of (1->4)-beta-D-xylans, to remove successive D-xylose residues from the non-reducing termini.By similarity
    Hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei303 – 3031By similarity

    GO - Molecular functioni

    1. alpha-L-arabinofuranosidase activity Source: UniProtKB-EC
    2. xylan 1,4-beta-xylosidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. xylan catabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Polysaccharide degradation, Xylan degradation

    Protein family/group databases

    CAZyiGH3. Glycoside Hydrolase Family 3.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-xylosidase/alpha-L-arabinofuranosidase 2Imported1 Publication
    Alternative name(s):
    Xylan 1,4-beta-xylosidase/Alpha-L-arabinofuranosidase 2
    Short name:
    MsXyl21 Publication
    Including the following 2 domains:
    Alternative name(s):
    1,4-beta-D-xylan xylohydrolaseBy similarity
    Xylan 1,4-beta-xylosidase
    Alpha-L-arabinofuranosidase (EC:3.2.1.55)
    Short name:
    ArabinosidaseBy similarity
    Gene namesi
    Name:Xyl2Imported
    OrganismiMedicago sativa subsp. varia (Alfalfa) (Medicago varia)
    Taxonomic identifieri36902 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeTrifolieaeMedicago

    Subcellular locationi

    Secretedextracellular spaceextracellular matrix By similarity

    GO - Cellular componenti

    1. proteinaceous extracellular matrix Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Extracellular matrix, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3333Sequence AnalysisAdd
    BLAST
    Chaini34 – 774741Beta-xylosidase/alpha-L-arabinofuranosidase 2Sequence AnalysisPRO_0000392641Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi136 – 1361N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi437 – 4371N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Structurei

    3D structure databases

    ProteinModelPortaliA5JTQ3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycoside hydrolase 3 family.Sequence Analysis

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di3.20.20.300. 1 hit.
    3.40.50.1700. 1 hit.
    InterProiIPR026891. Fn3-like.
    IPR026892. Glyco_hydro_3.
    IPR002772. Glyco_hydro_3_C.
    IPR001764. Glyco_hydro_3_N.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR30620. PTHR30620. 1 hit.
    PfamiPF14310. Fn3-like. 1 hit.
    PF00933. Glyco_hydro_3. 1 hit.
    PF01915. Glyco_hydro_3_C. 1 hit.
    [Graphical view]
    PRINTSiPR00133. GLHYDRLASE3.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    SSF52279. SSF52279. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    A5JTQ3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASVENRTPN VSVFLCFFVL FATLLLSGGR VSSQTSAVFA CDVAKNPALA    50
    NYGFCNKKLS VDARVKDLVR RLTLQEKVGN LVNSAVDVSR LGIPKYEWWS 100
    EALHGVSNIG PGTHFSNVIP GATSFPMPIL IAASFNASLF QTIGKVVSTE 150
    ARAMHNVGLA GLTYWSPNIN IFRDPRWGRG QETPGEDPLL ASKYAAGYVK 200
    GLQQTDDGDS NKLKVAACCK HYTAYDVDDW KGVQRYTFNA VVTQQDLDDT 250
    YQPPFKSCVI DGNVASVMCS YNQVNGKPTC ADPDLLKGVI RGKWKLNGYI 300
    VSDCDSVDVL FKNQHYTKTP EEAAAKSILA GLDLNCGSFL GRYTEGAVKQ 350
    GLIGEASINN AVYNNFATLM RLGFFDGDPS KQPYGNLGPK DVCTSANQEL 400
    AREAARQGIV LLKNCAGSLP LNAKAIKSLA VIGPNANATR AMIGNYEGIP 450
    CKYTSPLQGL TALVPTSFAA GCPDVQCTNA ALDDAKKIAA SADATVIVVG 500
    ANLAIEAESH DRINILLPGQ QQQLVTEVAN VAKGPVILAI MSGGGMDVSF 550
    AKTNKKITSI LWVGYPGEAG GAAIADVIFG YHNPSGRLPM TWYPQSYVDK 600
    VPMTNMNMRP DPATGYPGRT YRFYKGETVF SFGDGISYST FEHKLVKAPQ 650
    LVSVPLAEDH VCRSSKCKSL DVVGEHCQNL AFDIHLRIKN KGKMSSSQTV 700
    FLFSTPPAVH NAPQKHLLAF EKVLLTGKSE ALVSFKVDVC KDLGLVDELG 750
    NRKVALGKHM LHVGDLKHPL SVMI 774
    Length:774
    Mass (Da):83,383
    Last modified:June 26, 2007 - v1
    Checksum:iA709EB1AA6B3EF42
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EF569969 mRNA. Translation: ABQ45228.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EF569969 mRNA. Translation: ABQ45228.1 .

    3D structure databases

    ProteinModelPortali A5JTQ3.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH3. Glycoside Hydrolase Family 3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.20.20.300. 1 hit.
    3.40.50.1700. 1 hit.
    InterProi IPR026891. Fn3-like.
    IPR026892. Glyco_hydro_3.
    IPR002772. Glyco_hydro_3_C.
    IPR001764. Glyco_hydro_3_N.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    PANTHERi PTHR30620. PTHR30620. 1 hit.
    Pfami PF14310. Fn3-like. 1 hit.
    PF00933. Glyco_hydro_3. 1 hit.
    PF01915. Glyco_hydro_3_C. 1 hit.
    [Graphical view ]
    PRINTSi PR00133. GLHYDRLASE3.
    SUPFAMi SSF51445. SSF51445. 1 hit.
    SSF52279. SSF52279. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of a bifunctional beta-xylosidase/alpha-L-arabinosidase from alfalfa roots: heterologous expression in Medicago truncatula and substrate specificity of the purified enzyme."
      Xiong J.S., Balland-Vanney M., Xie Z.P., Schultze M., Kondorosi A., Kondorosi E., Staehelin C.
      J. Exp. Bot. 58:2799-2810(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. A21 Publication.
      Tissue: Root noduleImported.

    Entry informationi

    Entry nameiXYL2_MEDSV
    AccessioniPrimary (citable) accession number: A5JTQ3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 23, 2010
    Last sequence update: June 26, 2007
    Last modified: October 1, 2014
    This is version 27 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Multifunctional enzyme

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3